Hi-Plex HPLC Columns from Agilent

Agilent's Hi-Plex HPLC column provides reliable carbohydrate analysis with simple instrument requirements. Unlike Ion Chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated polystyrene gel impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58.

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Part Number	Short Description	Phase Type	Phase	Particle Size	ID	Length	Type	Price
PL1170-6820	DG-Hi-Plex Pb 300 x 7.7mm 8um Column	Carbohydrates	Hi-Plex Pb	8 um	7.7 mm	300 mm	Column	£1,169.00
PL1F70-6850	Hi-Plex Ca (Duo) 300 x 6.5mm 8um Column	Carbohydrates	Hi-Plex Ca	8 um	6.5 mm	300 mm	Column	£1,169.00
PL1670-0850	Hi-Plex Ca (Duo) 5 x 3mm 8um Guard Cartridge 2pk	Carbohydrates	Hi-Plex Ca	8 um	3.0 mm	5 mm	Guard	£209.00
PL1170-1850	Hi-Plex Ca (Duo) 50 x 7.7mm 8um Guard Column	Carbohydrates	Hi-Plex Ca	8 um	7.7 mm	50 mm	Guard	£362.00
PL1570-6810	Hi-Plex Ca 250 x 4.6mm 8um Column	Carbohydrates	Hi-Plex Ca	8 um	4.6 mm	250 mm	Column	£1,054.00
PL1170-6810	Hi-Plex Ca 300 x 7.7mm 8um Column	Carbohydrates	Hi-Plex Ca	8 um	7.7 mm	300 mm	Column	£1,169.00
PL1670-0810	Hi-Plex Ca 5 x 3mm 8um Guard Cartridge 2pk	Carbohydrates	Hi-Plex Ca	8 um	3.0 mm	5 mm	Guard	£213.00
PL1170-1810	Hi-Plex Ca 50 x 7.7mm 8um Guard Column	Carbohydrates	Hi-Plex Ca	8 um	7.7 mm	50 mm	Guard	£362.00
PL1570-5810	Hi-Plex Ca USP L19 250 x 4mm 8um Column	Carbohydrates	Hi-Plex Ca	8 um	4.0 mm	250 mm	Column	£778.00
PL1570-6830	Hi-Plex H 250 x 4.6mm 8um Column	Carbohydrates	Hi-Plex H	8 um	4.6 mm	250 mm	Column	£1,054.00
PL1F70-6830	Hi-Plex H 300 x 6.5mm 8um Column	Carbohydrates	Hi-Plex H	8 um	6.5 mm	300 mm	Column	£1,169.00
PL1170-6830	Hi-Plex H 300 x 7.7mm 8um Column	Carbohydrates	Hi-Plex H	8 um	7.7 mm	300 mm	Column	£1,169.00
PL1670-0830	Hi-Plex H 5 x 3mm 8um Guard Cartridge 2pk	Carbohydrates	Hi-Plex H	8 um	3.0 mm	5 mm	Guard	£213.00
PL1170-1830	Hi-Plex H 50 x 7.7mm 8um Guard Column	Carbohydrates	Hi-Plex H	8 um	7.7 mm	50 mm	Guard	£362.00
PL1170-2823	Hi-Plex H USP L17 100 x 7.7mm 8um Column	Carbohydrates	Hi-Plex H	8 um	7.7 mm	100 mm	Column	£925.00
PL1170-6860	Hi-Plex K 300 x 7.7mm 8um Column	Carbohydrates	Hi-Plex K	8 um	7.7 mm	300 mm	Column	£1,169.00
PL1670-0860	Hi-Plex K 5 x 3mm 8um Guard Cartridge 2pk	Carbohydrates	Hi-Plex K	8 um	3.0 mm	5 mm	Guard	£209.00
PL1170-1860	Hi-Plex K 50 x 7.7mm 8um Guard Column	Carbohydrates	Hi-Plex K	8 um	7.7 mm	50 mm	Guard	£362.00
PL1170-6840	Hi-Plex Na (Octo) 300 x 7.7mm 8um Column	Carbohydrates	Hi-Plex Na	8 um	7.7 mm	300 mm	Column	£1,169.00
PL1670-0840	Hi-Plex Na (Octo) 5 x 3mm 8um Guard Cartridge 2pk	Carbohydrates	Hi-Plex Na	8 um	3.0 mm	5 mm	Guard	£209.00
PL1170-1840	Hi-Plex Na (Octo) 50 x 7.5mm 8um Guard Column	Carbohydrates	Hi-Plex Na	8 um	7.5 mm	50 mm	Guard	£362.00
PL1171-6140	Hi-Plex Na 300 x 7.7mm 10um Column	Carbohydrates	Hi-Plex Na	10 um	7.7 mm	300 mm	Column	£1,169.00
PL1671-0140	Hi-Plex Na 5 x 3mm 10um Guard Cartridge 2pk	Carbohydrates	Hi-Plex Na	10 um	3.0 mm	5 mm	Guard	£209.00
PL1171-1140	Hi-Plex Na 50 x 7.7mm 10um Guard Column	Carbohydrates	Hi-Plex Na	10 um	7.7 mm	50 mm	Guard	£362.00
PL1670-0820	Hi-Plex Pb 5 x 3mm 8um Guard Cartridge 2pk	Carbohydrates	Hi-Plex Pb	8 um	3.0 mm	5 mm	Guard	£213.00
PL1170-1820	Hi-Plex Pb 50 x 7.7mm 8um Guard Column	Carbohydrates	Hi-Plex Pb	8 um	7.7 mm	50 mm	Guard	£362.00
PL1170-2820	Hi-Plex Pb USP L34 100 x 7.7mm 8um Column	Carbohydrates	Hi-Plex Pb	8 um	7.7 mm	100 mm	Column	£925.00

Features

Agilent's recommended column for accurate analysis of carbohydrates
Operates on a standard HPLC and does not require specialized equipment
Long column life and excellent batch-to-batch reproducibility
Wide range of counterions and column configurations to optimize resolution
Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % ACN as a modifier
Available in 8 µm and 10 µm particle sizes
Complies with USP L17, USP L19, USP L34, and USP L58

Specifications

Bonded Phase	Temperature Range	Flow Rate	Eluent	USP Designation
Hi-Plex Ca	80-90°C	0.6 mL/min	Water	L19
Hi-Plex Ca USP L19	80-90°C	0.3 mL/min	Water	L19
Hi-Plex Pb	70-90°C	0.6 mL/min	Water	L34
Hi-Plex H for carbohydrates	60-70°C	0.6 mL/min	Water	L17
Hi-Plex H for organic acids	40-60°C	0.6 mL/min	Dilute Acid	L17
Hi-Plex Ca (Duo)	80-90°C	0.6 mL/min	Water	L19
Hi-Plex K	80-90°C	0.6 mL/min	Water	-
Hi-Plex Na (Octo)	80-90°C	0.6 mL/min	Water, Sodium Hydroxide	L58
Hi-Plex Na	80-90°C	0.3 mL/min	Water	L58

Literature

Hi-Plex Brochure (486.38 kB)

Confident, lower-pressure analysis of carbohydrates, alcohols, and organic acids

Agilent Hi-Plex Application Compendium (7.22 MB)

Analysis of carbohydrates, alcohols, and organic acids