Affinity Columns
Affinity chromatography is one of the most effective ways to determinate the titer of monoclonal antibodies. Protein A and Protein G affinity chromatography columns absorb the IgG molecules onto the affinity resins which contain either immobilized protein A ligand or protein G ligand. Then, the remaining impurities and by-products from the fermentation broth can be removed. Elution of the purified monoclonal antibody and quantification by comparing the peak area to a calibration curve allows rapid measurement of the protein concentration.
