Agilent AdvanceBio HIC Columns
Agilent AdvanceBio HIC columns deliver robust and reproducible high-resolution separations of native proteins at the intact level. Built using the capabilities of ZORBAX fully porous particles and proprietary bonding technology, AdvanceBio HIC columns provide new levels of hydrophobicity and selectivity to address particularly challenging molecules such as monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), and other recombinant proteins. Together with the Agilent 1260 Infinity II bio-inert LC system, AdvanceBio HIC columns provide uncompromised performance and data consistency during characterization and validation.
- Optimized selectivity – Ideal for mAb oxidation and ADC DAR ratios
- Single chemistry – Reduces the need for multiple-column screening for different CQAs
- Enhanced robustness – Improved column lifetime for ultimate confidence in your data
- Proven performance – Every batch of media is tested with NIST mAb
- High quality – Each column is individually tested to ensure packing efficiency
- Greater productivity – Shorter columns reduce analysis time while maintaining separation performance
| AdvanceBio HIC | |
| Separation Mode | Hydrophobic Interaction (HIC) |
| Particle Type | Fully porous |
| Particle Size | 3.5 µm |
| Maximum Temperature | 60°C |
| pH Range | 2 - 8 |
| Pressure Rating | 400 bar |
| Hardware | Stainless steel |
AdvanceBio HIC Brochure
(1.79 MB)
Robust hydrophobic interaction chromatography of intact native proteins
ADC Workflow Consumables Ordering Guide
(1.41 MB)
Improving the quality of antibody drug conjugates by orthogonal analytical methods