Agilent AdvanceBio SEC Columns

AdvanceBio SEC HPLC Columns from Agilent

AdvanceBio SEC columns deliver accurate, precise quantitation for mAb aggregation and protein analysis. These innovative size exclusion chromatography columns were designed and engineered by Agilent to improve lab productivity by providing robust, reliable methods that eliminate sample reanalysis. Consistent results are achieved from column to column, batch to batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.

The AdvanceBio SEC 1.9 µm column is designed to improve resolution and provided faster separation of mAb aggregates and fragments by using both HPLC and UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility and stability, as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 µm column is intended to meet the need for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.

Agilent AdvanceBio SEC columns ensure easy, fast, and accurate quantitation for mAb aggregation and fragment analysis. AdvanceBio SEC offers two different particle sizes, 2.7 and 1.9 µm. These innovative size exclusion chromatography (SEC) columns were designed and engineered by Agilent to achieve high resolution and efficiency separation, with a robust and reliable performance. Consistent results are obtained from column to column, batch-to-batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.

The AdvanceBio SEC 1.9 µm columns are designed to improve resolution and provided faster separation of protein aggregates and fragments by UHPLC. The optimized sub 2 µm particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility, stability as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 μm column is intended to meet the needs for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
PL1980-3250PK	AdvanceBio SEC 120A 2.1 x 150mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	150 mm	120 A	PEEK Column	£1,438.00
PL1980-1250PK	AdvanceBio SEC 120A 2.1 x 50mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	50 mm	120 A	PEEK Column	£1,329.00
PL1580-3250	AdvanceBio SEC 120A 4.6 x 150mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	150 mm	120 A	Column	£1,858.00
PL1580-3250K	AdvanceBio SEC 120A 4.6 x 150mm 1.9um Method Validation Kit	AdvanceBio SEC	1.9 um	4.6 mm	150 mm	120 A	Validation Kit	£5,018.00
PL1580-1250	AdvanceBio SEC 120A 4.6 x 30 mm 1.9um Guard Column	AdvanceBio SEC	1.9 um	4.6 mm	30 mm	120 A	Guard	£564.00
PL1580-5250	AdvanceBio SEC 120A 4.6 x 300mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	300 mm	120 A	Column	£2,642.00
PL1580-5250K	AdvanceBio SEC 120A 4.6 x 300mm 1.9um Method Validation Kit	AdvanceBio SEC	1.9 um	4.6 mm	300 mm	120 A	Validation Kit	£6,882.00
PL1580-3350	AdvanceBio SEC 130A 4.6 x 150mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	150 mm	130 A	Column	£1,040.00
PL1580-5350	AdvanceBio SEC 130A 4.6 x 300mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	300 mm	130 A	Column	£1,433.00
PL1580-1350	AdvanceBio SEC 130A 4.6 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	4.6 mm	50 mm	130 A	Guard	£532.00
PL1180-3350	AdvanceBio SEC 130A 7.8 x 150mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	150 mm	130 A	Column	£1,186.00
PL1180-5350	AdvanceBio SEC 130A 7.8 x 300mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	300 mm	130 A	Column	£1,636.00
PL1180-1350	AdvanceBio SEC 130A 7.8 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	7.8 mm	50 mm	130 A	Guard	£532.00
PL1980-3201PK	AdvanceBio SEC 200A 2.1 x 150mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	150 mm	200 A	PEEK Column	£1,438.00
PL1980-1201PK	AdvanceBio SEC 200A 2.1 x 50mm 1.9um PEEK	AdvanceBio SEC	1.9 um	2.1 mm	50 mm	200 A	PEEK Column	£1,329.00
PL1580-3201	AdvanceBio SEC 200A 4.6 x 150mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	150 mm	200 A	Column	£1,858.00
PL1580-5201	AdvanceBio SEC 200A 4.6 x 300mm 1.9um	AdvanceBio SEC	1.9 um	4.6 mm	300 mm	200 A	Column	£2,642.00
PL1580-1201	AdvanceBio SEC 200A 4.6 x 30mm 1.9um Guard	AdvanceBio SEC	1.9 um	4.6 mm	30 mm	200 A	Guard	£564.00
PL1580-3301	AdvanceBio SEC 300A 4.6 x 150mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	150 mm	300 A	Column	£1,040.00
PL1580-5301	AdvanceBio SEC 300A 4.6 x 300mm 2.7um	AdvanceBio SEC	2.7 um	4.6 mm	300 mm	300 A	Column	£1,433.00
PL1580-1301	AdvanceBio SEC 300A 4.6 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	4.6 mm	50 mm	300 A	Guard	£532.00
PL1180-3301	AdvanceBio SEC 300A 7.8 x 150mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	150 mm	300 A	Column	£1,186.00
PL1180-5301	AdvanceBio SEC 300A 7.8 x 300mm 2.7um	AdvanceBio SEC	2.7 um	7.8 mm	300 mm	300 A	Column	£1,636.00
PL1180-1301	AdvanceBio SEC 300A 7.8 x 50mm 2.7um Guard	AdvanceBio SEC	2.7 um	7.8 mm	50 mm	300 A	Guard	£532.00

Features

High resolution, high-efficiency separation, and accurate quantitation for aggregates and fragments analysis
Prevented secondary interactions with mAb, ADC, and other proteins
Assured consistent column performance to eliminate rework
Enhanced column lifetime and productivity to meet robust CQA analysis
Specially designed standards for AdvanceBio SEC columns, providing optimal calibration and performance verification
AdvanceBio SEC 300 Å 2.7 µm is intended for separation protein ranging from 5,000 to 1.25 million Da including mAb fragments, mAb monomer, dimer, and high-level aggregates
AdvanceBio SEC 130 Å 2.7 µm is designed for small therapeutics molecular weight ranging from 100 to 120,000 Da
AdvanceBio SEC 200 Å 1.9 µm is suited for analyzing protein ranging from 2,000 to 700,000 Da including mAb monomer, dimmer, and LMW protein fragments
AdvanceBio SEC 120 Å 1.9 µm is best suited for analysis of peptides and small therapeutic proteins in the range 100 to 80,000 Da

Specifications

	AdvanceBio SEC
Particle size	1.9 µm	1.9 µm	2.7 µm	2.7 µm
Pore Size	120 Å	200 Å	130 Å	300 Å
Particle Type	Fully Porous	Fully Porous	Fully Porous	Fully Porous
Molecular Weight Lower Limit	1000 Da	2000 Da	100 Da	5000 Da
Molecular Weight Upper Limit	80,000 Da	700,000 Da	120,000 Da	1,250,000 Da
Temperature Limit	80°C	80°C	80°C	80°C
pH range	2 - 8.5	2 - 8.5	2 - 8.5	2 - 8.5

Literature

AdvanceBio 2.7 µm SEC Brochure (1.68 MB)

End uncertainty in monoclonal antibody characterization with AdvanceBio 2.7 µm columns

AdvanceBio SEC 120A 1.9um Brochure (1.60 MB)

High-resolution analysis of peptides and proteins with AdvanceBio SEC 120 Å 1.9 µm columns

AdvanceBio SEC 200A 1.9um Brochure (1.68 MB)

Fast separations for aggregates and fragments with AdvanceBio SEC 200 Å 1.9 µm columns

Aggregate Analysis Application Compendium (6.82 MB)

Collection of Agilent app notes on aggregate/fragment analysis

mAb Derived Therapeutics using Native SEC Workflow Ordering Guide (230.20 kB)

Workflow ordering guide will help you quickly choose and order the right columns, standards, reagents, and supplies for native SEC analysis of monoclonal antibodies

mAb Fragments and Dimers using SEC Workflow Ordering Guide (295.43 kB)

Workflow ordering guide will help you quickly choose and order the right columns, standards, reagents, and supplies for SEC analysis of mAb fragments and dimers

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis