Agilent Bio-Monolith Ion Exchange Columns
Agilent Bio-Monolith Ion Exchange columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages, and other macro biomolecules. The product family offers strong cation-exchange, strong and weak anion-exchange, and Protein A phases. Bio-Monolith IEX columns deliver flow rate-independent separation, speeding up method development time and decreasing costs.These columns are compatible with the Agilent InfinityLab LC instrument series.
Available phases include:
Bio-Monolith QA - quaternary amine bonded phase (strong anion-exchange) is fully charged over a working pH range of 2–13, binding negatively charged biomolecules
Bio-Monolith DEAE - diethylaminoethyl bonded phase (weak anion-exchange) offers increased selectivity of biomolecules with negative charge over a working pH range of 3–9
Bio-Monolith SO3 - sulfonyl bonded phase (strong cation-exchange) is fully charged over a working pH range of 2–13, binding positively charged biomolecules
- Polymer-based, monolith HPLC columns designed for macro biomolecule separations
- Flow-rate independent separations; no diffusion, no pores, and no void volume make transport between mobile and stationary phase very rapid
- Monolith disk is 5.2 x 4.95 mm (100 μL column volume) with continuous channels, eliminating diffusion mass transfer
- Extremely fast separations speed up method development time and decrease costs; locking in method parameters takes significantly less time and buffer
| Bio-Monolith QA | Bio-Monolith DEAE | Bio-Monolith SO3 | |
| Phase | Strong Anion Exchange | Weak Anion Exchange | Strong Cation Exchange |
| Particle Type | Monolith | Monolith | Monolith |
| Dimensions | 5.2 x 4.95 mm | 5.2 x 4.95 mm | 5.2 x 4.95 mm |
| Column Volume | 100 µL | 100 µL | 100 µL |
| Maximum Pressure | 150 bar | 150 bar | 150 bar |
| Temperature Range | Operating: 2 – 40°C Storage: 2 – 8°C |
Operating: 2 – 40°C Storage: 2 – 8°C |
Operating: 2 – 40°C Storage: 2 – 8°C |
| pH Range | 2 - 11 | 2 - 11 | 2 - 11 |
| Hardware | Stainless Steel | Stainless Steel | Stainless Steel |
| Colour Ring Identifier | Blue | Green | Red |
| Key Applications | – Adenovirus process monitoring and quality control – IgM purification monitoring and quality control – Monitoring DNA impurity removal – Monitoring endotoxin removal – HSA purity |
– Process monitoring and quality control of bacteriophage manufacturing and purification – Process monitoring and quality control of plasmid DNA purification |
– Fast and high resolution analytical separations of large molecules such as proteins and antibodies – Hemoglobin A1c fast analytics |
Charge Variant Analysis Application Compendium
(10.49 MB)