Agilent bio-Monolith Protein A and Protein G Affinity Columns
Agilent Bio-Monolith Protein A and Protein G affinity columns are designed for analytical separation of all IgG subclasses (human and mouse). Delivering fast and accurate quantitation of mAb titer (sub 1 minute) and purification of small amounts of mAb for subsequent Critical Quality Attribute (CQA) analysis by another complementary technique, such as aggregate analysis or charge variant analysis, they can easily be combined into a 2D-LC workflow.
Bio-Monolith Protein A and Protein G affinity chromatography columns are compatible with HPLC and UHPLC systems, including the Agilent 1100, 1200, 1260, and 1290 Bio-inert Quaternary LC Systems.
- High throughput clonal selection (60 seconds per sample)
- Robust mAb recovery at 97%
- Analytical separation of all IgG subclasses (human and mouse), Protein G offers alternate selectivity for those IgG molecules that do not bind to Protein A
- Flow-rate independent separations
- No diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid
- Extremely fast separations speed up method development time and decrease costs
- Key applications are quantitative determination of IgG (fermentation titer calculation)
| Bio-Monolith Protein A | Bio-monolith rProtein | Bio-Monolith Protein G | |
| Immobilised Ligand | Protein A from Staphylococcus aureusis | r-Protein A from Escherichia coli | r-Protein G from Escherichia coli |
| Particle Type | Monolith | Monolith | Monolith |
| Dimensions | 5.2 x 4.95 mm | 5.2 x 4.95 mm | 5.2 x 4.95 mm |
| Column Volume | 100 µL | 100 µL | 100 µL |
| Maximum Pressure | 150 bar | 150 bar | 150 bar |
| Maximum Temperature | 40°C | 40°C | 40°C |
| pH Range | 2 - 11 | 2 - 11 | 2 - 11 |
| Hardware | Stainless Steel | Stainless Steel | Stainless Steel |
Bio Monolith Protein A, rProtein A and Protein G Data Sheet
(180.16 kB)
Affinity columns designed for fast separation, isolation, and quantitation of antibodies
Titer Determination Application Compendium
(1.90 MB)
Collection of Agilent app notes for titer determination using Bio-Monolith Protein A and Protein G columns