Agilent PL-SAX Columns

Agilent PL-SAX columns are ideal for the anion-exchange HPLC separations of proteins, peptides, and deprotected synthetic oligonucleotides under denaturing conditions. The strong anion-exchange functionality, covalently linked to a chemically stable fully porous polymer, extends the operating pH range. In addition, the anion-exchange capacity is independent of pH.

For synthetic oligonucleotides, separations using denaturing conditions of temperature, organic solvent, and high pH are all possible. PL-SAX delivers improved chromatography for self-complementary or G-rich sequences that may associate to form aggregates or hairpin structures. The 5 μm material provides high-efficiency separations of n and n-1 sequences.

A wide range of particle sizes and column geometries permits analysis scale-up to purification. The strong anion-exchange functionality provides a material with exceptional chemical and thermal stability, even with sodium hydroxide eluents, leading to long column lifetime.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
PL1251-3102	PL-SAX 1000A 10um 150 x 25mm Column	PL-SAX	10 um	25.0 mm	150 mm	1000 A		£3,249.00
PL1551-3102	PL-SAX 1000A 10um 150 x 4.6mm Column	PL-SAX	10 um	4.6 mm	150 mm	1000 A	Column	£684.00
PL1751-3102	PL-SAX 1000A 10um 150 x 50mm Column	PL-SAX	10 um	50.0 mm	150 mm	1000 A		£10,863.00
PL1551-5102	PL-SAX 1000A 10um 250 x 4.6mm Column	PL-SAX	10 um	4.6 mm	250 mm	1000 A	Column	£812.00
PL1851-2102	PL-SAX 1000A 10um 300 x 100mm Column	PL-SAX	10 um	100.0 mm	300 mm	1000 A		£30,478.00
PL1651-0102	PL-SAX 1000A 10um 5 x 3mm Guard Cartridge 2pk	PL-SAX	10 um	3.0 mm	5 mm	1000 A	Guard	£121.00
PL1251-1102	PL-SAX 1000A 10um 50 x 25mm Column	PL-SAX	10 um	25.0 mm	50 mm	1000 A		£2,501.00
PL1251-3702	PL-SAX 1000A 30um 150 x 25mm Column	PL-SAX	30 um	25.0 mm	150 mm	1000 A		£2,731.00
PL1551-3702	PL-SAX 1000A 30um 150 x 4.6mm Column	PL-SAX	30 um	4.6 mm	150 mm	1000 A	Column	£575.00
PL1751-3702	PL-SAX 1000A 30um 150 x 50mm Column	PL-SAX	30 um	50.0 mm	150 mm	1000 A		£9,204.00
PL1551-5702	PL-SAX 1000A 30um 250 x 4.6mm Column	PL-SAX	30 um	4.6 mm	250 mm	1000 A	Column	£694.00
PL1851-3102	PL-SAX 1000A 30um 300 x 100mm Column	PL-SAX	30 um	100.0 mm	300 mm	1000 A		£27,352.00
PL1951-1502	PL-SAX 1000A 5um 50 x 2.1mm Column	PL-SAX	5 um	2.1 mm	50 mm	1000 A	Column	£603.00
PL1551-1502	PL-SAX 1000A 5um 50 x 4.6mm Column	PL-SAX	5 um	4.6 mm	50 mm	1000 A	Column	£603.00
PL1551-1502PK	PL-SAX 1000A 5um 50 x 4.6mm PEEK Column	PL-SAX	5 um	4.6 mm	50 mm	1000 A	Peek Column	£603.00
PL1951-3802	PL-SAX 1000A 8um 150 x 2.1mm Column	PL-SAX	8 um	2.1 mm	150 mm	1000 A	Column	£721.00
PL1251-3802	PL-SAX 1000A 8um 150 x 25mm Column	PL-SAX	8 um	25.0 mm	150 mm	1000 A		£3,488.00
PL1551-3802	PL-SAX 1000A 8um 150 x 4.6mm Column	PL-SAX	8 um	4.6 mm	150 mm	1000 A	Column	£721.00
PL1751-3802	PL-SAX 1000A 8um 150 x 50mm Column	PL-SAX	8 um	50.0 mm	150 mm	1000 A		£11,411.00
PL1151-3802	PL-SAX 1000A 8um 150 x 7.5mm Column	PL-SAX	8 um	7.5 mm	150 mm	1000 A	Column	£1,176.00
PL1951-1802	PL-SAX 1000A 8um 50 x 2.1mm Column	PL-SAX	8 um	2.1 mm	50 mm	1000 A	Column	£557.00
PL1551-1802	PL-SAX 1000A 8um 50 x 4.6mm Column	PL-SAX	8 um	4.6 mm	50 mm	1000 A	Column	£557.00
PL1151-1802	PL-SAX 1000A 8um 50 x 7.5mm Column	PL-SAX	8 um	7.5 mm	50 mm	1000 A	Column	£896.00
PL1251-3103	PL-SAX 4000A 10um 150 x 25mm Column	PL-SAX	10 um	25.0 mm	150 mm	4000 A		£3,249.00
PL1551-3103	PL-SAX 4000A 10um 150 x 4.6mm Column	PL-SAX	10 um	4.6 mm	150 mm	4000 A	Column	£684.00
PL1751-3103	PL-SAX 4000A 10um 150 x 50mm Column	PL-SAX	10 um	50.0 mm	150 mm	4000 A		£10,863.00
PL1551-5103	PL-SAX 4000A 10um 250 x 4.6mm Column	PL-SAX	10 um	4.6 mm	250 mm	4000 A	Column	£812.00
PL1851-2103	PL-SAX 4000A 10um 300 x 100mm Column	PL-SAX	10 um	100.0 mm	300 mm	4000 A		£30,478.00
PL1251-1103	PL-SAX 4000A 10um 50 x 25mm Column	PL-SAX	10 um	25.0 mm	50 mm	4000 A		£2,501.00
PL1251-3703	PL-SAX 4000A 30um 150 x 25mm Column	PL-SAX	30 um	25.0 mm	150 mm	4000 A		£2,731.00
PL1551-3703	PL-SAX 4000A 30um 150 x 4.6mm Column	PL-SAX	30 um	4.6 mm	150 mm	4000 A	Column	£575.00
PL1751-3703	PL-SAX 4000A 30um 150 x 50mm Column	PL-SAX	30 um	50.0 mm	150 mm	4000 A		£9,204.00
PL1551-5703	PL-SAX 4000A 30um 250 x 4.6mm Column	PL-SAX	30 um	4.6 mm	250 mm	4000 A	Column	£694.00
PL1851-3103	PL-SAX 4000A 30um 300 x 100mm Column	PL-SAX	30 um	100.0 mm	300 mm	4000 A		£27,352.00
PL1951-1503	PL-SAX 4000A 5um 50 x 2.1mm Column	PL-SAX	5 um	2.1 mm	50 mm	4000 A	Column	£603.00
PL1551-1503	PL-SAX 4000A 5um 50 x 4.6mm Column	PL-SAX	5 um	4.6 mm	50 mm	4000 A	Column	£603.00
PL1951-3803	PL-SAX 4000A 8um 150 x 2.1mm Column	PL-SAX	8 um	2.1 mm	150 mm	4000 A	Column	£721.00
PL1551-3803	PL-SAX 4000A 8um 150 x 4.6mm Column	PL-SAX	8 um	4.6 mm	150 mm	4000 A	Column	£721.00
PL1151-3803	PL-SAX 4000A 8um 150 x 7.5mm Column	PL-SAX	8 um	7.5 mm	150 mm	4000 A	Column	£1,176.00
PL1951-1803	PL-SAX 4000A 8um 50 x 2.1mm Column	PL-SAX	8 um	2.1 mm	50 mm	4000 A	Column	£557.00
PL1551-1803	PL-SAX 4000A 8um 50 x 4.6mm Column	PL-SAX	8 um	4.6 mm	50 mm	4000 A	Column	£557.00
PL1151-1803	PL-SAX 4000A 8um 50 x 7.5mm Column	PL-SAX	8 um	7.5 mm	50 mm	4000 A	Column	£896.00

Features

Anion-exchange purifications over a wider pH range extend applications
HPLC flow rates and rapid equilibration reduce purification cycle times
Small particles deliver excellent chromatographic performance
Wide range of particle sizes and two pore sizes for flexible analysis to scale up purification
1000 Å pore size for high-capacity purifications
4000 Å gigaporous particles with improved mass transfer for large biomolecules and high-speed, high-resolution purifications
Exceptional stability for long column lifetime

Specifications

	PL-SAX
Phase	Strong Anion Exchange
Particle Type	Fully Porous PS/DVB particles
Particle Size	5 µm, 8 µm, 10 µm, and 30 µm
Pore Size	1000 Å and 4000 Å
pH Range	1 – 14
Maximum Temperature	80°C
Hardware	Stainless Steel

Literature

PL-SAX Oligonucleotide Workflow Ordering Guide (499.85 kB)

Oligonucleotide purification using anion exchange liquid chromatography

Charge Variant Analysis Application Compendium (10.49 MB)

Collection of Agilent app notes on charge variant analysis

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis

Agilent PL-SAX Columns

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