Agilent Poroshell 300 Columns

Poroshell 300 HPLC Columns from Agilent

Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks.

Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. But Poroshell columns use a unique particle made with a layer of porous silica on a solid core of silica.

This reduces the diffusion distance for proteins making practical rapid HPLC separations of peptides and proteins up to 500 – 1,000 kDA. Poroshell 300SB C18, C8, C3, and 300Extend columns provide excellent stability and selectivity choices with TFA and formic acid mobile faces.

Poroshell 300Extend C18 columns can be used at varying pH levels for unique separations and additional selectivity choices.

Agilent Poroshell 300 columns are ideal for fast separations of proteins and peptides because the 5 μm superficially porous particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. Poroshell columns with StableBond bonded phases provide excellent stability and selectivity choices with TFA and formic acid mobile phases. The Poroshell 300Extend-C18 column can be used from pH 2 - 11 for unique separations. These columns can also be used for analytical protein separations and LC/MS separations.

Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. However, Poroshell columns use a superficially porous particle made with a thin layer of porous silica, 0.25 μm thick, on a solid core of silica. This reduces the diffusion distance for proteins, making rapid HPLC separations of peptides and proteins up to 500 – 1000 kDa possible with 400/600 bar HPLC systems, including the Agilent 1260 Infinity II bio-inert LC.

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Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
671750-902	Poroshell 300 Extend C18, 5um, 1 x 75mm, Column	C18	Poroshell Extend C18	L1	5 um	1.0 mm	75 mm	300 A	Column	£882.00
670750-902	Poroshell 300 Extend C18, 5um, 2.1 x 75mm, Column	C18	Poroshell Extend C18	L1	5 um	2.1 mm	75 mm	300 A	L1	£873.00
5185-5968	Poroshell 300 SB-C18, 5um, 1 x 17mm, Guard 3pk	C18	Poroshell SB-C18	L1	5 um	1.0 mm	17 mm	300 A	Guard	£442.00
661750-902	Poroshell 300 SB-C18, 5um, 1 x 75mm, Column	C18	Poroshell SB-C18	L1	5 um	1.0 mm	75 mm	300 A	Column	£882.00
821075-920	Poroshell 300 SB-C18, 5um, 2.1 x 5mm, Guard, 4pk	C18	Poroshell SB-C18	L1	5 um	2.1 mm	12.5 mm	300 A	L1	£298.00
660750-902	Poroshell 300 SB-C18, 5um, 2.1 x 75mm, Column	C18	Poroshell SB-C18	L1	5 um	2.1 mm	75 mm	300 A	L1	£882.00
822600-909	Poroshell 300 SB-C3, 5um, 1 x 30mm, Column	C3	Poroshell SB-C3	L56	5 um	1.0 mm	30 mm	300 A	Column	£827.00
661750-909	Poroshell 300 SB-C3, 5um, 1 x 75mm, Column	C3	Poroshell SB-C3	L56	5 um	1.0 mm	75 mm	300 A	Column	£882.00
821075-924	Poroshell 300 SB-C3, 5um, 2.1 x 12.5mm, Guard, 4pk	C3	Poroshell SB-C3	L56	5 um	2.1 mm	12.5 mm	300 A	L56	£298.00
660750-909	Poroshell 300 SB-C3, 5um, 2.1 x 75mm, Column	C3	Poroshell SB-C3	L56	5 um	2.1 mm	75 mm	300 A	L56	£882.00
661750-906	Poroshell 300 SB-C8, 5um, 1 x 75mm, Column	C8	Poroshell SB-C8	L7	5 um	1.0 mm	75 mm	300 A	Column	£882.00
821075-918	Poroshell 300 SB-C8, 5um, 2.1 x 12.5mm, Guard, 4pk	C8	Poroshell SB-C8	L7	5 um	2.1 mm	12.5 mm	300 A	L7	£298.00
660750-906	Poroshell 300 SB-C8, 5um, 2.1 x 75mm, Column	C8	Poroshell SB-C8	L7	5 um	2.1 mm	75 mm	300 A	L7	£882.00

Features

UHPLC separations of biomolecules with superficially porous particles
300 Å pore provide high efficiency and recovery with proteins (up to 1000 kDa) and monoclonal antibodies
Achieve long lifetime at low pH with Poroshell 300SB; at high pH with Poroshell 300Extend-C18
Optimize recovery and selectivity with four different bonded phases – 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18

Specifications

	Poroshell 300SB-C18	Poroshell 300SB-C8	Poroshell 300SB-C3	Poroshell 300Extend-C18
Bonded Phase	C18	C8	C3	C18
Particle Type	Superficially Porous	Superficially Porous	Superficially Porous	Superficially Porous
Endcapped	No	No	No	Yes
Particle Size	5 µm	5 µm	5 µm	5 µm
Pore Size	300 Å	300 Å	300 Å	300 Å
Temperature Limit	90°C	90°C	90°C	40°C above pH 8, 60°C below pH 8
pH Range	1 - 8	1 - 8	1 - 8	2 - 11
USP Designation	L1	L7	L56	L1

Literature

AdvanceBio Reversed Phase Columns White Paper (3.07 MB)

Better characterization of biomolecules using Agilent AdvanceBio reversed phase columns

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis