Agilent PROTEEMA SEC Columns

Agilent PROTEEMA columns are designed for aqueous SEC separations of natural and synthetic proteins, peptides, and enzymes with molecular weights ranging from 100 to 7,500,000 Da. The columns feature a diol stationary phase that meets USP L20 specifications. Columns are available with a choice of porosities (100 Å, 300 Å, and 1000 Å), and two particle sizes (3 µm and 5 µm) to meet a variety of application needs. Bioinert hardware is offered to increase sensitivity and recovery for demanding applications, and PROTEEMA Lux columns are recommended for use with light scattering detectors.

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Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
PRA080505	PROTEEMA Guard Column, 5um, 8 x 50mm	PROTEEMA	5 um	8.0 mm	50 mm		Guard	£431.00
PRA080505BI	PROTEEMA BioInert Guard Column, 5um, 8 x 50mm	PROTEEMA	5 um	8.0 mm	50 mm		Guard	£586.00
PRA080505LS	PROTEEMA Lux Guard Column, 5um, 8 x 50mm	PROTEEMA Lux	5 um	8.0 mm	50 mm		Guard	£489.00
PRA0815053E2	PROTEEMA Column, 300A, 5um, 8 x 150mm	PROTEEMA	5 um	8.0 mm	150 mm	300 A	Column	£1,296.00
PRA0815053E2BI	PROTEEMA BioInert Column, 300A, 5um, 8 x 150mm	PROTEEMA	5 um	8.0 mm	150 mm	300 A	Bioinert Column	£1,711.00
PRA0830051E2	PROTEEMA Column, 100A, 5um, 8 x 300mm	PROTEEMA	5 um	8.0 mm	300 mm	100 A	Column	£1,432.00
PRA0830051E2BI	PROTEEMA BioInert Column, 100A, 5um, 8 x 300mm	PROTEEMA	5 um	8.0 mm	300 mm	100 A	Bioinert Column	£1,847.00
PRA0830051E2LS	PROTEEMA Lux Column, 100A, 5um, 8 x 300mm	PROTEEMA Lux	5 um	8.0 mm	300 mm	100 A	Column	£1,603.00
PRA0830051E3	PROTEEMA Column, 1000A, 5um, 8 x 300mm	PROTEEMA	5 um	8.0 mm	300 mm	1000 A	Column	£1,432.00
PRA0830051E3BI	PROTEEMA BioInert Column, 1000A, 5um, 8 x 300mm	PROTEEMA	5 um	8.0 mm	300 mm	1000 A	Bioinert Column	£1,847.00
PRA0830051E3LS	PROTEEMA Lux Column, 1000A, 5um, 8 x 300mm	PROTEEMA Lux	5 um	8.0 mm	300 mm	1000 A	Column	£1,603.00
PRA0830053E2	PROTEEMA Column, 300A, 5um, 8 x 300mm	PROTEEMA	5 um	8.0 mm	300 mm	300 A	Column	£1,432.00
PRA0830053E2BI	PROTEEMA BioInert Column, 300A, 5um, 8 x 300mm	PROTEEMA	5 um	8.0 mm	300 mm	300 A	Bioinert Column	£1,847.00
PRA0830053E2LS	PROTEEMA Lux Column, 300A, 5um, 8 x 300mm	PROTEEMA Lux	5 um	8.0 mm	300 mm	300 A	Column	£1,603.00
PRM050303	PROTEEMA Guard Column, 3um, 4.6 x 30mm	PROTEEMA	3 um	4.6 mm	30 mm		Guard	£431.00
PRM050303BI	PROTEEMA BioInert Guard Column, 3um, 4.6 x 30mm	PROTEEMA	3 um	4.6 mm	30 mm		Guard	£586.00
PRM0525031E2	PROTEEMA Column, 100A, 3um, 4.6 x 250mm	PROTEEMA	3 um	4.6 mm	250 mm	100 A	Column	£1,595.00
PRM0525031E2BI	PROTEEMA BioInert Column, 100A, 3um, 4.6 x 250mm	PROTEEMA	3 um	4.6 mm	250 mm	100 A	Bioinert Column	£2,010.00
PRM0525033E2	PROTEEMA Column, 300A, 3um, 4.6 x 250mm	PROTEEMA	3 um	4.6 mm	250 mm	300 A	Column	£1,595.00
PRM0525033E2BI	PROTEEMA BioInert Column, 300A, 3um, 4.6 x 250mm	PROTEEMA	3 um	4.6 mm	250 mm	300 A	Bioinert Column	£2,010.00
PRM0525053E2	PROTEEMA Column, 300A, 5um, 4.6 x 250mm	PROTEEMA	5 um	4.6 mm	250 mm	300 A	Column	£1,595.00
PRM0525053E2BI	PROTEEMA BioInert Column, 300A, 5um, 4.6 x 250mm	PROTEEMA	5 um	4.6 mm	250 mm	300 A	Bioinert Column	£2,010.00

Features

Diol SEC chemistry that meets USP L20 classification
Recommended for analysis of proteins, peptides and enzymes
Compatible with a wide range of aqueous eluents with an optimum performance when used in pH range 2.0 to 7.5
Shipped in water with 7.5 mM sodium azide to prevent microbial growth
Narrow particle size distribution that contributes to excellent efficiency
Excellent stability under a wide range of physical, chemical, and biological environments
Guard columns protect and extend the lifetime of analytical columns
Choice of stainless steel or bioinert hardware
PROTEEMA Lux is recommended for use with light scattering detectors

Specifications

	PROTEEMA
Pore Size	100 Å	300 Å	1000 Å
Bonded Phase	Diol	Diol	Diol
Particle Type	Fully Porous	Fully Porous	Fully Porous
Molecular Weight Lower Limit	3 µm, 5 µm	3 µm, 5 µm	5 µm
Molecular Weight Upper Limit	100 Da	1000 Da	1000 Da
Temperature Limit	150,000 Da	1,200,000 Da	7,500,000 Da
pH Range	2 - 8	2 - 8	2 - 8
Shipping Solvent	Water	Water	Water
USP Designation	L20	L20	L20

Literature

Agilent GPC/SEC Columns and Consumables Brochure (1.08 MB)

Uncompromising analysis of polymers and macromolecules with the expanded portfolio of Agilent GPC/SEC columns and standards

Agilent PROTEEMA Column User Guide (1.66 MB)

Instructions and best practices for using the Agilent PROTEEMA SEC columns

Agilent GPC/SEC eBook (2.16 MB)

What you should know when you need to analyze polymers, biopolymers, and proteins