Agilent ZORBAX 300StableBond Columns

Zorbax 300Stablebond HPLC Columns from Agilent

Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC-MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.

These columns are available in five different bonded phases (C18, C8, C3, Diphenyl and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.

Agilent ZORBAX 300StableBond columns are an ideal choice for the reproducible separations of proteins and peptides. The 300 Å wide pores provide efficient separation of proteins and peptides by allowing analytes to completely access the bonded phase. The 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC-MS separations at low pH, these columns can also be used with formic acid and acetic acid mobile phase modifiers.

ZORBAX 300StableBond columns are available in four different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides.

ZORBAX 300SB-C18 – bonding a sterically-protected C18 functionality, diisobutyl n-octadecylsilane, to the ZORBAX particle produces a chemically stable stationary phase for long column lifetimes when using acidic mobile phases for the analysis of peptide fragments from protein digests and small proteins.

ZORBAX 300SB-C8 – the sterically protected C8 functionality, diisopropyl n-octylsilane, gives improved separation and recovery for larger proteins, including antibodies.

ZORBAX 300SB-C3 – broadens the selectivity, peak shape and recovery options for larger proteins, including antibodies.

ZORBAX 300SB-CN – suitable for polar molecules at low pH or high temperature, provides low bleed and excellent peak shape.

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Buy

Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
895100-902	DG-PrepHT ZORBAX 300SB-C18 21.2 x 100mm 5um Cartridge	C18	ZORBAX SB-C18	L1	5 um	21.2 mm	100 mm	300 A	Cartridge	£1,895.00
895150-902	DG-PrepHT ZORBAX 300SB-C18 21.2 x 150mm 5um Cartridge	C18	ZORBAX SB-C18	L1	5 um	21.2 mm	150 mm	300 A	Cartridge	£2,629.00
897150-102	DG-PrepHT ZORBAX 300SB-C18 21.2 x 150mm 7um Cartridge	C18	ZORBAX SB-C18	L1	7 um	21.2 mm	150 mm	300 A	Cartridge	£2,290.00
897250-102	DG-PrepHT ZORBAX 300SB-C18 21.2 x 250mm 7um Cartridge	C18	ZORBAX SB-C18	L1	7 um	21.2 mm	250 mm	300 A	Cartridge	£3,017.00
820212-921	PrepHT ZORBAX 300SB-C18 15.8 x 10mm 5um Guard 2pk	C18	ZORBAX SB-C18	L1	5 um	15.8 mm	10 mm	300 A	Guard	£315.00
895050-902	PrepHT ZORBAX 300SB-C18 21.2 x 50mm 5um Cartridge	C18	ZORBAX SB-C18	L1	5 um	21.2 mm	50 mm	300 A	Cartridge	£1,313.00
820212-924	PrepHT ZORBAX 300SB-C3 15.8 x 10mm 5um Guard 2pk	C3	ZORBAX SB-C3	L56	5 um	15.8 mm	10 mm	300 A	Guard	£315.00
895100-909	PrepHT ZORBAX 300SB-C3 21.2 x 100mm 5um Cartridge	C3	ZORBAX SB-C3	L56	5 um	21.2 mm	100 mm	300 A	Cartridge	£1,895.00
895150-909	PrepHT ZORBAX 300SB-C3 21.2 x 150mm 5um Cartridge	C3	ZORBAX SB-C3	L56	5 um	21.2 mm	150 mm	300 A	Cartridge	£2,629.00
897150-109	PrepHT ZORBAX 300SB-C3 21.2 x 150mm 7um Cartridge	C3	ZORBAX SB-C3	L56	7 um	21.2 mm	150 mm	300 A	Cartridge	£2,290.00
897250-109	PrepHT ZORBAX 300SB-C3 21.2 x 250mm 7um Cartridge	C3	ZORBAX SB-C3	L56	7 um	21.2 mm	250 mm	300 A	Cartridge	£3,017.00
895050-909	PrepHT ZORBAX 300SB-C3 21.2 x 50mm 5um Cartridge	C3	ZORBAX SB-C3	L56	5 um	21.2 mm	50 mm	300 A	Cartridge	£1,313.00
820212-918	PrepHT ZORBAX 300SB-C8 15.8 x 10mm 5um Guard 2pk	C8	ZORBAX SB-C8	L7	5 um	15.8 mm	10 mm	300 A	Guard	£315.00
895100-906	PrepHT ZORBAX 300SB-C8 21.2 x 100mm 5um Cartridge	C8	ZORBAX SB-C8	L7	5 um	21.2 mm	100 mm	300 A	Cartridge	£1,895.00
895150-906	PrepHT ZORBAX 300SB-C8 21.2 x 150mm 5um Cartridge	C8	ZORBAX SB-C8	L7	5 um	21.2 mm	150 mm	300 A	Cartridge	£2,629.00
897150-106	PrepHT ZORBAX 300SB-C8 21.2 x 150mm 7um Cartridge	C8	ZORBAX SB-C8	L7	7 um	21.2 mm	150 mm	300 A	Cartridge	£2,290.00
897250-106	PrepHT ZORBAX 300SB-C8 21.2 x 250mm 7um Cartridge	C8	ZORBAX SB-C8	L7	7 um	21.2 mm	250 mm	300 A	Cartridge	£3,017.00
895050-906	PrepHT ZORBAX 300SB-C8 21.2 x 50mm 5um Cartridge	C8	ZORBAX SB-C8	L7	5 um	21.2 mm	50 mm	300 A	Cartridge	£1,313.00
897250-105	PrepHT ZORBAX 300SB-CN 21.2 x 250mm 7um Cartridge	Cyano	ZORBAX SB-CN	L10	7 um	21.2 mm	250 mm	300 A	Cartridge	£3,017.00
5064-8267	ZORBAX 300 SB-C18 0.3 x 150mm 3.5um Column	C18	ZORBAX SB-C18	L1	3.5 um	0.3 mm	150 mm	300 A	Column	£616.00
5065-9913	ZORBAX 300 SB-C18 0.3 x 5mm 5um Cartridge 5pk	C18	ZORBAX SB-C18	L1	5 um	0.3 mm	5 mm	300 A	Cartridge	£371.00
5064-8268	ZORBAX 300 SB-C18 0.5 x 150mm 3.5um Column	C18	ZORBAX SB-C18	L1	3.5 um	0.5 mm	150 mm	300 A	Column	£616.00
5064-8264	ZORBAX 300 SB-C18 0.5 x 150mm 5um Column	C18	ZORBAX SB-C18	L1	5 um	0.5 mm	150 mm	300 A	Column	£555.00
863630-902	ZORBAX 300 SB-C18 1 x 150mm 3.5um Column	C18	ZORBAX SB-C18	L1	3.5 um	1.0 mm	150 mm	300 A	Column	£598.00
861630-902	ZORBAX 300 SB-C18 1 x 250mm 5um Column	C18	ZORBAX SB-C18	L1	5 um	1.0 mm	250 mm	300 A	Column	£598.00
865630-902	ZORBAX 300 SB-C18 1 x 50mm 3.5um Column	C18	ZORBAX SB-C18	L1	3.5 um	1.0 mm	50 mm	300 A	Column	£421.00
858750-902	ZORBAX 300 SB-C18 2.1 x 100mm 1.8um	C18	ZORBAX SB-C18	L1	1.8 um	2.1 mm	100 mm	300 A	Column	£726.00
861775-902	ZORBAX 300 SB-C18 2.1 x 100mm 3.5um	C18	ZORBAX SB-C18	L1	3.5 um	2.1 mm	100 mm	300 A	Column	£534.00
883750-902	ZORBAX 300 SB-C18 2.1 x 150mm 5um	C18	ZORBAX SB-C18	L1	5 um	2.1 mm	150 mm	300 A	Column	£534.00
881750-902	ZORBAX 300 SB-C18 2.1 x 250mm 5um	C18	ZORBAX SB-C18	L1	5 um	2.1 mm	250 mm	300 A	Column	£586.00
857750-902	ZORBAX 300 SB-C18 2.1 x 50mm 1.8um	C18	ZORBAX SB-C18	L1	1.8 um	2.1 mm	50 mm	300 A	Column	£659.00
865750-902	ZORBAX 300 SB-C18 2.1 x 50mm 3.5um	C18	ZORBAX SB-C18	L1	3.5 um	2.1 mm	50 mm	300 A	Column	£394.00
863974-302	ZORBAX 300 SB-C18 3 x 150mm 3.5um	C18	ZORBAX SB-C18	L1	3.5 um	3.0 mm	150 mm	300 A	Column	£593.00
861973-902	ZORBAX 300 SB-C18 4.6 x 100mm 3.5um	C18	ZORBAX SB-C18	L1	3.5 um	4.6 mm	100 mm	300 A	Column	£534.00
820950-921	ZORBAX 300 SB-C18 4.6 x 12.5mm 5um Guard 4pk	C18	ZORBAX SB-C18	L1	5 um	4.6 mm	12.5 mm	300 A	Guard	£194.00
863973-902	ZORBAX 300 SB-C18 4.6 x 150mm 3.5um	C18	ZORBAX SB-C18	L1	3.5 um	4.6 mm	150 mm	300 A	Column	£586.00
883995-902	ZORBAX 300 SB-C18 4.6 x 150mm 5um	C18	ZORBAX SB-C18	L1	5 um	4.6 mm	150 mm	300 A	Column	£534.00
880995-902	ZORBAX 300 SB-C18 4.6 x 250mm 5um	C18	ZORBAX SB-C18	L1	5 um	4.6 mm	250 mm	300 A	Column	£586.00
865973-902	ZORBAX 300 SB-C18 4.6 x 50mm 3.5um	C18	ZORBAX SB-C18	L1	3.5 um	4.6 mm	50 mm	300 A	Column	£394.00
860950-902	ZORBAX 300 SB-C18 4.6 x 50mm 5um	C18	ZORBAX SB-C18	L1	5 um	4.6 mm	50 mm	300 A	Column	£374.00
821125-924	ZORBAX 300 SB-C3 2.1 x 12.5mm 5um Guard 4pk	C3	ZORBAX SB-C3	L56	5 um	2.1 mm	12.5 mm	300 A	Guard	£194.00
883750-909	ZORBAX 300 SB-C3 2.1 x 150mm 5um	C3	ZORBAX SB-C3	L56	5 um	2.1 mm	150 mm	300 A	Column	£534.00
863974-309	ZORBAX 300 SB-C3 3 x 150mm 3.5um	C3	ZORBAX SB-C3	L56	3.5 um	3.0 mm	150 mm	300 A	Column	£593.00
820950-924	ZORBAX 300 SB-C3 4.6 x 12.5mm 5um Guard 4pk	C3	ZORBAX SB-C3	L56	5 um	4.6 mm	12.5 mm	300 A	Guard	£194.00
863973-909	ZORBAX 300 SB-C3 4.6 x 150mm 3.5um	C3	ZORBAX SB-C3	L56	3.5 um	4.6 mm	150 mm	300 A	Column	£586.00
883995-909	ZORBAX 300 SB-C3 4.6 x 150mm 5um	C3	ZORBAX SB-C3	L56	5 um	4.6 mm	150 mm	300 A	Column	£534.00
880995-909	ZORBAX 300 SB-C3 4.6 x 250mm 5um	C3	ZORBAX SB-C3	L56	5 um	4.6 mm	250 mm	300 A	Column	£586.00
865973-909	ZORBAX 300 SB-C3 4.6 x 50mm 3.5um	C3	ZORBAX SB-C3	L56	3.5 um	4.6 mm	50 mm	300 A	Column	£394.00
860950-909	ZORBAX 300 SB-C3 4.6 x 50mm 5um	C3	ZORBAX SB-C3	L56	5 um	4.6 mm	50 mm	300 A	Column	£374.00
863630-906	ZORBAX 300 SB-C8 1 x 150mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	1.0 mm	150 mm	300 A	Column	£598.00
865630-906	ZORBAX 300 SB-C8 1 x 50mm 3.5um Column	C8	ZORBAX SB-C8	L7	3.5 um	1.0 mm	50 mm	300 A	Column	£421.00
861775-906	ZORBAX 300 SB-C8 2.1 x 100mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	2.1 mm	100 mm	300 A	Column	£534.00
821125-918	ZORBAX 300 SB-C8 2.1 x 12.5mm 5um Guard 4pk	C8	ZORBAX SB-C8	L7	5 um	2.1 mm	12.5 mm	300 A	Guard	£194.00
863750-906	ZORBAX 300 SB-C8 2.1 x 150mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	2.1 mm	150 mm	300 A	Column	£586.00
883750-906	ZORBAX 300 SB-C8 2.1 x 150mm 5um	C8	ZORBAX SB-C8	L7	5 um	2.1 mm	150 mm	300 A	Column	£534.00
865750-906	ZORBAX 300 SB-C8 2.1 x 50mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	2.1 mm	50 mm	300 A	Column	£394.00
884950-565	ZORBAX 300 SB-C8 3 x 100mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	3.0 mm	100 mm	300 A	Column	£534.00
861973-306	ZORBAX 300 SB-C8 3 x 100mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	3.0 mm	100 mm	300 A	Column	£534.00
863974-306	ZORBAX 300 SB-C8 3 x 150mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	3.0 mm	150 mm	300 A	Column	£586.00
861973-906	ZORBAX 300 SB-C8 4.6 x 100mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	4.6 mm	100 mm	300 A	Column	£534.00
820950-918	ZORBAX 300 SB-C8 4.6 x 12.5mm 5um Guard 4pk	C8	ZORBAX SB-C8	L7	5 um	4.6 mm	12.5 mm	300 A	Guard	£194.00
863973-906	ZORBAX 300 SB-C8 4.6 x 150mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	4.6 mm	150 mm	300 A	Column	£586.00
883995-906	ZORBAX 300 SB-C8 4.6 x 150mm 5um	C8	ZORBAX SB-C8	L7	5 um	4.6 mm	150 mm	300 A	Column	£529.00
880995-906	ZORBAX 300 SB-C8 4.6 x 250mm 5um	C8	ZORBAX SB-C8	L7	5 um	4.6 mm	250 mm	300 A	Column	£580.00
865973-906	ZORBAX 300 SB-C8 4.6 x 50mm 3.5um	C8	ZORBAX SB-C8	L7	3.5 um	4.6 mm	50 mm	300 A	Column	£394.00
860950-906	ZORBAX 300 SB-C8 4.6 x 50mm 5um	C8	ZORBAX SB-C8	L7	5 um	4.6 mm	50 mm	300 A	Column	£374.00
883750-905	ZORBAX 300 SB-CN 2.1 x 150mm 5um	Cyano	ZORBAX SB-CN	L10	5 um	2.1 mm	150 mm	300 A	Column	£529.00
820950-923	ZORBAX 300 SB-CN 4.6 x 12.5mm 5um Guard 4pk	Cyano	ZORBAX SB-CN	L10	5 um	4.6 mm	12.5 mm	300 A	Guard	£194.00
863973-905	ZORBAX 300 SB-CN 4.6 x 150mm 3.5um	Cyano	ZORBAX SB-CN	L10	3.5 um	4.6 mm	150 mm	300 A	Column	£580.00
883995-905	ZORBAX 300 SB-CN 4.6 x 150mm 5um	Cyano	ZORBAX SB-CN	L10	5 um	4.6 mm	150 mm	300 A	Column	£529.00
880995-905	ZORBAX 300 SB-CN 4.6 x 250mm 5um	Cyano	ZORBAX SB-CN	L10	5 um	4.6 mm	250 mm	300 A	Column	£580.00
865973-905	ZORBAX 300 SB-CN 4.6 x 50mm 3.5um	Cyano	ZORBAX SB-CN	L10	3.5 um	4.6 mm	50 mm	300 A	Column	£394.00
860950-905	ZORBAX 300 SB-CN 4.6 x 50mm 5um	Cyano	ZORBAX SB-CN	L10	5 um	4.6 mm	50 mm	300 A	Column	£365.00
863750-902	ZORBAX RRHD 300 SB-C18 2.1 x 150mm 1.8um	C18	ZORBAX SB-C18	L1	1.8 um	2.1 mm	150 mm	300 A	Column	£952.00
858750-909	ZORBAX RRHD 300 SB-C3 2.1 x 100mm 1.8um	C3	ZORBAX SB-C3	L56	1.8 um	2.1 mm	100 mm	300 A	Column	£726.00
863750-914	ZORBAX RRHD 300 SB-C3 2.1 x 150mm 1.8um	C3	ZORBAX SB-C3	L56	1.8 um	2.1 mm	150 mm	300 A	Column	£1,098.00
857750-909	ZORBAX RRHD 300 SB-C3 2.1 x 50mm 1.8um	C3	ZORBAX SB-C3	L56	1.8 um	2.1 mm	50 mm	300 A	Column	£659.00
858750-906	ZORBAX RRHD 300 SB-C8 2.1 x 100mm 1.8um	C8	ZORBAX SB-C8	L7	1.8 um	2.1 mm	100 mm	300 A	Column	£726.00
866750-906	ZORBAX RRHD 300 SB-C8 2.1 x 150mm 1.8um	C8	ZORBAX SB-C8	L7	1.8 um	2.1 mm	150 mm	300 A	Column	£1,098.00
857750-906	ZORBAX RRHD 300 SB-C8 2.1 x 50mm 1.8um	C8	ZORBAX SB-C8	L7	1.8 um	2.1 mm	50 mm	300 A	Column	£659.00

Features

Four different bonded phases (C18, C8, C3, and CN) for reversed-phase selectivity optimization of peptides and proteins
Exceptional stability and long lifetime at low pH with TFA containing mobile phases
Excellent reproducibility
Use up to 80°C to increase sample recovery and improve efficiency for difficult proteins
Available in capillary and nano column dimensions for proteomics and other LC-MS applications

Specifications

	ZORBAX 300SB-C18	ZORBAX 300SB-C8	ZORBAX 300SB-C3	ZORBAX 300SB-CN
Bonded Phase	C18	C8	C3	Cyano
Particle Type	Fully Porous	Fully Porous	Fully Porous	Fully Porous
Endcapped	No	No	No	No
Particle Size	1.8 µm, 3.5 µm, 5 µm	1.8 µm, 3.5 µm, 5 µm	3.5 µm, 5 µm	3.5 µm, 5 µm
Pore Size	300 Å	300 Å	300 Å	300 Å
Surface Area	45 m²/g	45 m²/g	45 m²/g	45 m²/g
Carbon Load	2.8%	1.5%	1.1%	1.2%
Temperature Limit	90°C	80°C	80°C	80°C
pH Range	1 - 8	1 - 8	1 - 8	1 - 8
USP Designation	L1	L7	L56	L10

Literature

AdvanceBio Reversed Phase Columns White Paper (3.07 MB)

Better characterization of biomolecules using Agilent AdvanceBio reversed phase columns

Intact and Subunit Purity Application Compendium (3.08 MB)

Collection of Agilent app notes for intact and subunit analysis

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis