Agilent AdvanceBio Oligonucleotide Columns

AdvanceBio Oligonucleotide HPLC Columns from Agilent

To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.

If your columns don’t provide enough resolution, you can’t be sure your results are accurate. If your columns are not robust, you will have to replace them frequently, which will disrupt your workflow and increase your costs. Agilent AdvanceBio oligonucleotide columns feature high efficiency, 2.7 µm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. What’s more, we test every batch of AdvanceBio oligonucleotide media with a resolution standard to ensure consistent performance.

The family of AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization of proteins, antibodies, conjugates, new biological entities and biopharmaceuticals.

Agilent AdvanceBio Oligonucleotide columns offer high-quality, high-resolution ion-pair reversed-phase (IP-RP) column chemistry platform that support analytical characterization through purification with seamless method scale-up. To successfully separate trityl-off, deprotected oligonucleotides, HPLC columns need to have high resolving power and be robust enough to withstand high pH and temperature, which provide better separation and denaturing conditions for optimal resolution and oligonucleotide separation.

AdvanceBio oligonucleotide columns feature high efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, the particles are chemically modified to make them exceedingly resistant to high pH mobile phases. They are bonded with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, every batch of AdvanceBio oligonucleotide media is tested with a resolution standard to ensure consistent performance.

AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities, and biopharmaceuticals.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
655750-702	AdvanceBio Oligonucleotide 2.1 x 100mm 2.7um Column	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	100 mm	120 A	Column	£799.00
653750-702	AdvanceBio Oligonucleotide 2.1 x 150mm 2.7um Column	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	150 mm	120 A	Column	£909.00
659750-702	AdvanceBio Oligonucleotide 2.1 x 50mm 2.7um Column	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	50 mm	120 A	Column	£650.00
821725-921	AdvanceBio Oligonucleotide 2.1 x 5mm 2.7um Fast Guard, 3pk	AdvanceBio Oligonucleotide	2.7 um	2.1 mm	5 mm	120 A	Guard	£559.00
671150-702	AdvanceBio Oligonucleotide 21.2 x 150mm 4um Column	AdvanceBio Oligonucleotide	4 um	21.2 mm	150 mm	120 A	Column	£3,300.00
671050-702	AdvanceBio Oligonucleotide 21.2 x 50mm 4um Column	AdvanceBio Oligonucleotide	4 um	21.2 mm	50 mm	120 A	Column	£1,835.00
655950-702	AdvanceBio Oligonucleotide 4.6 x 100mm 2.7um Column	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	100 mm	120 A	Column	£799.00
695971-702	AdvanceBio Oligonucleotide 4.6 x 100mm 4um Column	AdvanceBio Oligonucleotide	4 um	4.6 mm	100 mm	120 A	Column	£622.00
653950-702	AdvanceBio Oligonucleotide 4.6 x 150mm 2.7um Column	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	150 mm	120 A	Column	£909.00
693971-702	AdvanceBio Oligonucleotide 4.6 x 150mm 4um Column	AdvanceBio Oligonucleotide	4 um	4.6 mm	150 mm	120 A	Column	£757.00
659950-702	AdvanceBio Oligonucleotide 4.6 x 50mm 2.7um Column	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	50 mm	120 A	Column	£650.00
699971-702	AdvanceBio Oligonucleotide 4.6 x 50mm 4um Column	AdvanceBio Oligonucleotide	4 um	4.6 mm	50 mm	120 A	Column	£516.00
820750-921	AdvanceBio Oligonucleotide 4.6 x 5mm 2.7um Fast Guard, 3pk	AdvanceBio Oligonucleotide	2.7 um	4.6 mm	5 mm	120 A	Guard	£573.00
820750-941	AdvanceBio Oligonucleotide 4.6 x 5mm 4um Fast Guard, 3pk	AdvanceBio Oligonucleotide	4 um	4.6 mm	5 mm	120 A	Guard	£561.00

Features

Designed for ion-pair reversed-phase separation of the trityl-off, deprotected oligos using either TEAA or TEA-HFIP
Features the HPH-C18 robust column chemistry technology which is stable at elevated pH and temperatures
Streamlines scale up from analytical characterization to preparative purification
Every batch of AdvanceBio Oligonucleotide media is tested with the Agilent Oligonucleotide Resolution standard

Specifications

	AdvanceBio Oligonucleotide
Bonded Phase	C18
Particle Type	Superficially Porous
Endcapped	Double
Particle Size	2.7 µm, 4 µm
Pore Size	120 Å
Temp Limits	65°C
pH range	3 - 11

Literature

Agilent AdvanceBio Oligonucleotide Brochure (1.10 MB)

High resolution oligonucleotide separations with long column lifetime on HPLC and UHPLC systems

Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis