Thermo Scientific DNAPac RP Columns

Thermo Scientific DNAPac RP columns offer ion-pair reversed phase separations of nucleic acid mixtures. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs.

The DNAPac RP column is based on spherical wide pore 4 µm polymer resin. The phase is highly stable to extremes of pH (0 - 14), and temperature (5 - 110°C), and operates with mobile phases compatible with direct mass spectrometry. The column may be operated with both HPLC and UHPLC instruments, and resolves both small and very large dsDNA segments. The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over.