Peptide Analysis Columns
Peptide analysis columns are designed to provide high-resolution peptide maps for protein identification and determination of post-translation modifications. These columns let you quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence.
Reversed phase UHPLC/HPLC is the preferred technique for the analysis of peptide digests with either MS or UV detection. LC/MS is used for the identification of the peptide fragments and determination of sequence coverage whereas LC/UV is more commonly used for peptide map comparisons in the monitoring/QC segments.