Daicel Protein-based Chiral Columns

Protein Based Chiral Columns from Daicel

Columns packed with the immobilized protein stationary phases (PSPs) are successfully utilized by scientists for separation and characterization of chiral compounds in a great number of applications, ranging from drug discovery and quality assurance of marketed drugs to environmental monitoring.

Our protein-based chiral selectors are immobilized on 5 µm spherical silica particles. The PSPs function entirely in the reversed-phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values. Enantioselectivity can easily be controlled or improved by changes in the mobile phase composition: pH, buffer and organic modifier types and concentrations.

All PSP selectors are packed in columns with 2, 3, 4 and 10 mm internal diameter to cover separations on both analytical and semi-preparative scales.

Daicel CHIRALPAK protein-based chiral selectors are immobilised on 5 µm spherical silica particles. They operate in reversed phase mode, using buffers with low organic modifier content and at moderate pH values. Enantioselectivity can easily be controlled or improved by changes in the mobile phase composition.

Daicel protein stationary chiral selectors are available with three main proteins:
CHIRALPAK AGP – α 1-acid glycoprotein (USP L41)
CHIRALPAK HSA – human serum albumin (USP L79)
CHIRALPAK CBH – cellobiohydrolase (stable enzyme) (USP L108)

The Daicel CHIRALPAK AGP selector has extremely broad applicability for the separation of a wide variety of chiral compounds, such as amines, acids, and nonprolytes. The enantioselectivity of the Daicel CHIRALPAK CBH selector is complementary to that of the AGP selector.

A range of other serum albumin protein columns is available for use alongside CHIRALPAK HSA in binding studies to confirm the ability of a pharmaceutical to bind to a protein, including:
CHIRALPAK RSA – rat serum albumin
CHIRALPAK BSA – bovine serum albumin (USP L75)
CHIRALPAK MSA – mouse serum albumin
CHIRALPAK PSA – pig serum albumin
CHIRALPAK DSA – dog serum albumin

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Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Type	Price
30791	CHIRALPAK AGP 10 x 2mm 5um Guard Cartridge, 2pk	CHIRALPAK AGP	5 um	2.0 mm	10 mm	Guard	£222.79
30781	CHIRALPAK AGP 10 x 3mm 5um Guard Cartridge, 2pk	CHIRALPAK AGP	5 um	3.0 mm	10 mm	Guard	£222.79
30711	CHIRALPAK AGP 10 x 4mm 5um Guard Cartridge, 2pk	CHIRALPAK AGP	5 um	4.0 mm	10 mm	Guard	£255.97
30733	CHIRALPAK AGP 100 x 10mm 5um Semi-Prep Column	CHIRALPAK AGP	5 um	10.0 mm	100 mm	Semi-Prep Column	£4,299.75
30793	CHIRALPAK AGP 100 x 2.1mm 5um Analytical Column	CHIRALPAK AGP	5 um	2.1 mm	100 mm	Analytical Column	£1,355.71
30783	CHIRALPAK AGP 100 x 3mm 5um Analytical Column	CHIRALPAK AGP	5 um	3.0 mm	100 mm	Analytical Column	£1,232.47
30713	CHIRALPAK AGP 100 x 4mm 5um Analytical Column	CHIRALPAK AGP	5 um	4.0 mm	100 mm	Analytical Column	£1,232.47
30734	CHIRALPAK AGP 150 x 10mm 5um Semi-Prep Column	CHIRALPAK AGP	5 um	10.0 mm	150 mm	Semi-Prep Column	£6,252.89
30794	CHIRALPAK AGP 150 x 2.1mm 5um Analytical Column	CHIRALPAK AGP	5 um	2.1 mm	150 mm	Analytical Column	£1,772.86
30784	CHIRALPAK AGP 150 x 3mm 5um Analytical Column	CHIRALPAK AGP	5 um	3.0 mm	150 mm	Analytical Column	£1,616.43
30714	CHIRALPAK AGP 150 x 4mm 5um Analytical Column	CHIRALPAK AGP	5 um	4.0 mm	150 mm	Analytical Column	£1,616.43
30792	CHIRALPAK AGP 50 x 2.1mm 5um Analytical Column	CHIRALPAK AGP	5 um	2.1 mm	50 mm	Analytical Column	£1,061.82
30782	CHIRALPAK AGP 50 x 3mm 5um Analytical Column	CHIRALPAK AGP	5 um	3.0 mm	50 mm	Analytical Column	£971.75
30712	CHIRALPAK AGP 50 x 4mm 5um Analytical Column	CHIRALPAK AGP	5 um	4.0 mm	50 mm	Analytical Column	£971.75
35714	CHIRALPAK BSA 150 x 4mm 5um Analytical Column	CHIRALPAK BSA	5 um	4.0 mm	150 mm	Analytical Column	£2,682.99
35792	CHIRALPAK BSA 50 x 2.1mm 5um Analytical Column	CHIRALPAK BSA	5 um	2.1 mm	50 mm	Analytical Column	£1,635.39
35782	CHIRALPAK BSA 50 x 3mm 5um Analytical Column	CHIRALPAK BSA	5 um	3.0 mm	50 mm	Analytical Column	£1,635.39
35712	CHIRALPAK BSA 50 x 4mm 5um Analytical Column	CHIRALPAK BSA	5 um	4.0 mm	50 mm	Analytical Column	£1,635.39
33791	CHIRALPAK CBH 10 x 2mm 5um Guard Cartridge, 2pk	CHIRALPAK CBH	5 um	2.0 mm	10 mm	Guard	£222.79
33781	CHIRALPAK CBH 10 x 3mm 5um Guard Cartridge, 2pk	CHIRALPAK CBH	5 um	3.0 mm	10 mm	Guard	£222.79
33711	CHIRALPAK CBH 10 x 4mm 5um Guard Cartridge, 2pk	CHIRALPAK CBH	5 um	4.0 mm	10 mm	Guard	£255.97
33733	CHIRALPAK CBH 100 x 10mm 5um Semi-Prep Column	CHIRALPAK CBH	5 um	10.0 mm	100 mm	Semi-Prep Column	£4,299.75
33793	CHIRALPAK CBH 100 x 2.1mm 5um Analytical Column	CHIRALPAK CBH	5 um	2.1 mm	100 mm	Analytical Column	£1,355.71
33783	CHIRALPAK CBH 100 x 3mm 5um Analytical Column	CHIRALPAK CBH	5 um	3.0 mm	100 mm	Analytical Column	£1,232.47
33713	CHIRALPAK CBH 100 x 4mm 5um Analytical Column	CHIRALPAK CBH	5 um	4.0 mm	100 mm	Analytical Column	£1,232.47
33734	CHIRALPAK CBH 150 x 10mm 5um Semi-Prep Column	CHIRALPAK CBH	5 um	10.0 mm	150 mm	Semi-Prep Column	£6,252.89
33794	CHIRALPAK CBH 150 x 2.1mm 5um Analytical Column	CHIRALPAK CBH	5 um	2.1 mm	150 mm	Analytical Column	£1,772.86
33784	CHIRALPAK CBH 150 x 3mm 5um Analytical Column	CHIRALPAK CBH	5 um	3.0 mm	150 mm	Analytical Column	£1,616.43
33714	CHIRALPAK CBH 150 x 4mm 5um Analytical Column	CHIRALPAK CBH	5 um	4.0 mm	150 mm	Analytical Column	£1,616.43
33792	CHIRALPAK CBH 50 x 2.1mm 5um Analytical Column	CHIRALPAK CBH	5 um	2.1 mm	50 mm	Analytical Column	£1,061.82
33782	CHIRALPAK CBH 50 x 3mm 5um Analytical Column	CHIRALPAK CBH	5 um	3.0 mm	50 mm	Analytical Column	£971.75
33712	CHIRALPAK CBH 50 x 4mm 5um Analytical Column	CHIRALPAK CBH	5 um	4.0 mm	50 mm	Analytical Column	£971.75
36714	CHIRALPAK DSA 150 x 4mm 5um Analytical Column	CHIRALPAK DSA	5 um	4.0 mm	150 mm	Analytical Column	£5,712.01
36792	CHIRALPAK DSA 50 x 2.1mm 5um Analytical Column	CHIRALPAK DSA	5 um	2.1 mm	50 mm	Analytical Column	£2,962.66
36782	CHIRALPAK DSA 50 x 3mm 5um Analytical Column	CHIRALPAK DSA	5 um	3.0 mm	50 mm	Analytical Column	£2,962.66
36712	CHIRALPAK DSA 50 x 4mm 5um Analytical Column	CHIRALPAK DSA	5 um	4.0 mm	50 mm	Analytical Column	£3,597.86
34791	CHIRALPAK HSA 10 x 2mm 5um Guard Cartridge, 2pk	CHIRALPAK HSA	5 um	2.0 mm	10 mm	Guard	£203.83
34781	CHIRALPAK HSA 10 x 3mm 5um Guard Cartridge, 2pk	CHIRALPAK HSA	5 um	3.0 mm	10 mm	Guard	£203.83
34711	CHIRALPAK HSA 10 x 4mm 5um Guard Cartridge, 2pk	CHIRALPAK HSA	5 um	4.0 mm	10 mm	Guard	£237.01
34733	CHIRALPAK HSA 100 x 10mm 5um Semi-Prep Column	CHIRALPAK HSA	5 um	10.0 mm	100 mm	Semi-Prep Column	£4,299.75
34793	CHIRALPAK HSA 100 x 2.1mm 5um Analytical Column	CHIRALPAK HSA	5 um	2.1 mm	100 mm	Analytical Column	£1,161.36
34783	CHIRALPAK HSA 100 x 3mm 5um Analytical Column	CHIRALPAK HSA	5 um	3.0 mm	100 mm	Analytical Column	£1,038.12
34713	CHIRALPAK HSA 100 x 4mm 5um Analytical Column	CHIRALPAK HSA	5 um	4.0 mm	100 mm	Analytical Column	£1,038.12
34734	CHIRALPAK HSA 150 x 10mm 5um Semi-Prep Column	CHIRALPAK HSA	5 um	10.0 mm	150 mm	Semi-Prep Column	£6,252.89
34794	CHIRALPAK HSA 150 x 2.1mm 5um Analytical Column	CHIRALPAK HSA	5 um	2.1 mm	150 mm	Analytical Column	£1,426.82
34784	CHIRALPAK HSA 150 x 3mm 5um Analytical Column	CHIRALPAK HSA	5 um	3.0 mm	150 mm	Analytical Column	£1,322.53
34714	CHIRALPAK HSA 150 x 4mm 5um Analytical Column	CHIRALPAK HSA	5 um	4.0 mm	150 mm	Analytical Column	£1,322.53
34792	CHIRALPAK HSA 50 x 2.1mm 5um Analytical Column	CHIRALPAK HSA	5 um	2.1 mm	50 mm	Analytical Column	£971.75
34782	CHIRALPAK HSA 50 x 3mm 5um Analytical Column	CHIRALPAK HSA	5 um	3.0 mm	50 mm	Analytical Column	£886.43
34712	CHIRALPAK HSA 50 x 4mm 5um Analytical Column	CHIRALPAK HSA	5 um	4.0 mm	50 mm	Analytical Column	£886.43
37782	CHIRALPAK MSA 50 x 3mm 5um Analytical Column	CHIRALPAK MSA	5 um	3.0 mm	50 mm	Analytical Column	£2,948.44
37712	CHIRALPAK MSA 50 x 4mm 5um Analytical Column	CHIRALPAK MSA	5 um	4.0 mm	50 mm	Analytical Column	£3,597.86
43782	CHIRALPAK PSA 50 x 3mm 5um Analytical Column	CHIRALPAK PSA	5 um	3.0 mm	50 mm	Analytical Column	£2,948.44
43712	CHIRALPAK PSA 50 x 4mm 5um Analytical Column	CHIRALPAK PSA	5 um	4.0 mm	50 mm	Analytical Column	£3,597.86
38714	CHIRALPAK RSA 150 x 4mm 5um Analytical Column	CHIRALPAK RSA	5 um	4.0 mm	150 mm	Analytical Column	£3,597.86
38792	CHIRALPAK RSA 50 x 2.1mm 5um Analytical Column	CHIRALPAK RSA	5 um	2.1 mm	50 mm	Analytical Column	£1,796.56
38782	CHIRALPAK RSA 50 x 3mm 5um Analytical Column	CHIRALPAK RSA	5 um	3.0 mm	50 mm	Analytical Column	£1,796.56
38712	CHIRALPAK RSA 50 x 4mm 5um Analytical Column	CHIRALPAK RSA	5 um	4.0 mm	50 mm	Analytical Column	£1,796.56

Features

CHIRALPAK AGP has very broad applicability and is suitable for enantiomer resolution of all types of compounds, including amines (primary, secondary, tertiary, and quaternary ammonium compounds), strong and weak acids, and non-ionisable compounds.
CHIRALPAK HSA offers high enantioselectivity for compounds bearing carboxylic groups, including, strong and weak acids, zwitterionic molecules, and non-ionisable compounds.
CHIRALPAK CBH can offer high enantioselectivity for basic compounds, including primary and secondary amines, and non-ionisable compounds.

Specifications

	CHIRALPAK AGP	CHIRALPAK HSA	CHIRALPAK CBH
Chiral selector	α 1-acid glycoprotein	human serum albumin	cellobiohydrolase (stable enzyme)
USP	L41	L79	L108
Particle size	5 µm	5 µm	5 µm
Type	Immobilised protein-based	Immobilised protein-based	Immobilised protein-based
Separation mode	revesed phase	revesed phase	revesed phase
Shipping solvent	water/2-propanol (85/15)	water/2-propanol (90/10)	water/2-propanol (85/15)
Temperature range	20 - 30°C	20 - 30°C	20 - 30°C

Literature

Daicel Chiral Selectors Poster (279.53 kB)

Structures of commercially available Daicel chiral stationary phases

Daicel Product List 2024 (2.97 MB)

Catalogue of part numbers for Daicel columns

CHIRALPAK AGP, HSA and CBH Brochure (5.46 MB)

Immobilized protein stationary phases

CHIRALPAK AGP, CBH and HSA Screening Strategy (63.97 kB)

Screening Strategies for immobilised protein stationary phases to identify the best method to separate target compounds