Agilent SUPREMA Aqueous SEC Columns

Agilent SUPREMA columns offer an optimal solution for aqueous SEC analysis of both neutral and anionic polymers, with typical applications including PEO, PEG, pullulan, dextran, polyacrylamide, hyaluronic acid, polyacrylic acid, and carboxymethyl cellulose. These columns, based on acrylate copolymer, come in three particle sizes (3, 5, and 10 μm), with various porosities ranging from 30 to 30,000 Å and options for linear/mixed-bed configurations, to address application requirements across a molecular weight range of 100 to 30,000,000 Da. Additionally, SUPREMA columns meet USP L25, L37, L38, and L39 designations, show high tolerance to high buffer concentrations, temperatures up to 80°C, organic solvents (up to 100% methanol or acetonitrile), and pH range of 2 to 12.

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Buy

Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
SUA080503	SUPREMA Guard Column, 3um, 8 x 50mm	SUPREMA	3 um	8.0 mm	50 mm		Guard	£431.00
SUA080505	SUPREMA Guard Column, 5um, 8 x 50mm	SUPREMA	5 um	8.0 mm	50 mm		Guard	£431.00
SUA080510	SUPREMA Guard Column, 10um, 8 x 50mm	SUPREMA	10 um	8.0 mm	50 mm		Guard	£431.00
SUA0830031E2	SUPREMA Column, 100A, 3um, 8 x 300mm	SUPREMA	3 um	8.0 mm	300 mm	100 A	Column	£1,789.00
SUA0830031E3	SUPREMA Column, 1000A, 3um, 8 x 300mm	SUPREMA	3 um	8.0 mm	300 mm	1000 A	Column	£1,789.00
SUA0830033E1	SUPREMA Column, 30A, 3um, 8 x 300mm	SUPREMA	3 um	8.0 mm	300 mm	30 A	Column	£1,789.00
SUA0830051E2	SUPREMA Column, 100A, 5um, 8 x 300mm	SUPREMA	5 um	8.0 mm	300 mm	100 A	Column	£1,560.00
SUA0830051E3	SUPREMA Column, 1000A, 5um, 8 x 300mm	SUPREMA	5 um	8.0 mm	300 mm	1000 A	Column	£1,560.00
SUA0830053E1	SUPREMA Column, 30A, 5um, 8 x 300mm	SUPREMA	5 um	8.0 mm	300 mm	30 A	Column	£1,560.00
SUA083005LIM	SUPREMA Linear M Column, 5um, 8 x 300mm	SUPREMA	5 um	8.0 mm	300 mm	Mixed	Column	£1,727.00
SUA083005LIS	SUPREMA Linear S Column, 5um, 8 x 300mm	SUPREMA	5 um	8.0 mm	300 mm	Mixed	Column	£1,727.00
SUA0830101E2	SUPREMA Column, 100A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	100 A	Column	£1,370.00
SUA0830101E3	SUPREMA Column, 1000A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	1000 A	Column	£1,370.00
SUA0830101E4	SUPREMA Column, 10,000A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	10,000 A	Column	£1,370.00
SUA0830103E1	SUPREMA Column, 30A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	30 A	Column	£1,370.00
SUA0830103E2	SUPREMA Column, 300A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	300 A	Column	£1,370.00
SUA0830103E3	SUPREMA Column, 3000A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	3000 A	Column	£1,370.00
SUA0830103E4	SUPREMA Column, 30,000A, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	30,000 A	Column	£1,370.00
SUA083010LIM	SUPREMA Linear M Column, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	Mixed	Column	£1,506.00
SUA083010LUH	SUPREMA Ultrahigh Column, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	Mixed	Column	£1,506.00
SUA083010LXL	SUPREMA Linear XL Column, 10um, 8 x 300mm	SUPREMA	10 um	8.0 mm	300 mm	Mixed	Column	£1,506.00
SUM050305	SUPREMA Guard Column, 5um, 4.6 x 30mm	SUPREMA	5 um	4.6 mm	30 mm		Guard	£431.00
SUM0525051E2	SUPREMA Column, 100A, 5um, 4.6 x 250mm	SUPREMA	5 um	4.6 mm	250 mm	100 A	Column	£1,560.00
SUM0525051E3	SUPREMA Column, 1000A, 5um, 4.6 x 250mm	SUPREMA	5 um	4.6 mm	250 mm	1000 A	Column	£1,560.00
SUM0525053E1	SUPREMA Column, 30A, 5um, 4.6 x 250mm	SUPREMA	5 um	4.6 mm	250 mm	30 A	Column	£1,560.00
SUP2005	SUPREMA Guard Column, 10um, 20 x 50mm	SUPREMA	10 um	20.0 mm	50 mm		Guard	£966.00
SUP20301E2	SUPREMA Column, 100A, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	100 A	Column	£3,349.00
SUP20301E3	SUPREMA Column, 1000A, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	1000 A	Column	£3,349.00
SUP20301E4	SUPREMA Column, 10,000A, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	10,000 A	Column	£3,349.00
SUP20303E1	SUPREMA Column, 30A, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	30 A	Column	£3,349.00
SUP20303E3	SUPREMA Column, 3000A, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	3000 A	Column	£3,349.00
SUP2030LIM	SUPREMA Linear M Column, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	Mixed	Column	£3,679.00
SUP2030LXL	SUPREMA Linear XL Column, 10um, 20 x 300mm	SUPREMA	10 um	20.0 mm	300 mm	Mixed	Column	£3,679.00

Features

SUPREMA columns are packed with highly porous particles of a modified acrylate copolymer
Recommended for aqueous SEC separations of neutral and anionic polymers
Compatible with a wide range of aqueous eluents with optimum performance when used with eluents that have a pH in the range 6.0 to 10.2
Meets USP L25, L37, L38, and L39 classifications
Shipping solvent is water with 7.5 mM sodium azide to prevent microbial growth
Flexible configurations available in three particle sizes, 3, 5, and 10 μm, and various individual porosities, between 30, and 30,000 Å as well as different linear/mixed-bed columns
Excellent separation efficiency obtained by narrow particle size distribution and optimised frit technology
Columns manufactured under DIN EN ISO 9001 quality standards
Individually tested columns with a certificate of analysis available
Guard columns protect and extend the lifetime of analytical columns
SUPREMA Lux is recommended for light scattering detection

Specifications

	SUPREMA
Pore Size	30 Å	100 Å	300 Å	1000 Å	3000 Å	10,000 Å	30,000 Å	Mixed S	Mixed M	Mixed XL	Mixed Ultrahigh
Particle Type	Individual Pore Size	Individual Pore Size	Individual Pore Size	Individual Pore Size	Individual Pore Size	Individual Pore Size	Individual Pore Size	Mixed Pore Size	Mixed Pore Size	Mixed Pore Size	Mixed Pore Size
Molecular Weight Lower Limit	100 Da	100 Da	1000 Da	1000 Da	10,000 Da	10,000 Da	50,000 Da	100 Da	200 Da	200 Da	500 Da
Molecular Weight Upper Limit	30,000 Da	100,000 Da	300,000 Da	1,000,000 Da	3,000,000 Da	10,000,000 Da	30,000,000 Da	100,000 Da	1,000,000 Da	3,000,000 Da	30,000,000 Da
Particle Size	3 µm, 5 µm, 10 µm	3 µm, 5 µm, 10 µm	10 µm	3 µm, 5 µm, 10 µm	10 µm	10 µm	10 µm	5 µm	5 µm, 10 µm	10 µm	10 µm
Temperature Limit	80°C	80°C	80°C	80°C	80°C	80°C	80°C	80°C	80°C	80°C	80°C
Shipping Solvent	Water	Water	Water	Water	Water	Water	Water	Water	Water	Water	Water

Literature

Agilent GPC/SEC Columns and Consumables Brochure (1.08 MB)

Uncompromising analysis of polymers and macromolecules with the expanded portfolio of Agilent GPC/SEC columns and standards

Agilent SUPREMA Column User Guide (1.66 MB)

Instructions and best practices for using the Agilent SUPREMA GPC/SEC columns

Agilent GPC/SEC eBook (2.16 MB)

What you should know when you need to analyze polymers, biopolymers, and proteins