Agilent ZORBAX HILIC Plus HPLC Columns
Agilent ZORBAX HILIC Plus HPLC columns are non-bonded silica columns made with ZORBAX Eclipse Plus silica optimised for retention and separation of small polar analytes. These columns are manufactured with traditional fully porous particles in 3.5 µm for HPLC separations or 1.8 µm for UHPLC applications. The robustness and reproducibility of HILIC separations on ZORBAX columns help to switch from challenging, often MS-incompatible ion pair chromatography and normal phase applications.
- High-purity Type B silica for excellent peak shape especially for polar basic analytes
- Traditional fully porous material in 1.8 µm and 3.5 µm
- The high similarity of ZORBAX HILIC Plus and InfinityLab Poroshell 120 HILIC columns allows for easy method transfer to superficially porous column technology
- Fully compatible with Agilent InfinityLab deactivator additive, which improves sensitivity in challenging analyses of phosphorylated and carboxylated compounds with stainless steel LC/MS systems
- ZORBAX HILIC Plus is ideally suited for the retention of small, polar analytes and is recommended for EPA Method 1694
- Columns ship containing acetonitrile/water and are ready to use for HILIC separations
- 1.8 µm UHPLC guard columns to further extend the life of your analytical column (no additional holder required)
- USP L3 classification
| ZORBAX HILIC Plus | |
| Bonded Phase | Silica |
| Particle Type | Fully Porous |
| Particle Size | 1.8 µm and 3.5 µm |
| Pore Size | 95 Å |
| Surface Area | 160 m2/g |
| Endcapped | No |
| Maximum Temperature | 60°C |
| pH Range | 0 - 8 |
| Pressure Limit | 3.5 µm - 400 bar 1.8 µm - 1200 bar |
| Hardware | Stainless Steel |
| USP Designation | L3 |
HILIC Method Development Technical Overview
(1.82 MB)
Hydrophilic interaction chromatography method development and troubleshooting
