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Avantor® ACE® HILIC-B HPLC Columns

Avantor® ACE® HILIC-B HPLC columns have been designed specifically for HILIC applications and are suitable for the separation of polar compounds. The HILIC-B stationary phase is a proprietary aminopropyl with a basic character with an ionisable positive surface charge depending on the mobile phase pH.

The ACE HILIC-B columns form part of a range of three proprietary HILIC phases (HILIC-A, HILIC-B, and HILIC-N) with alternative selectivity to each other. Using a screening approach to method development it is easy to achieve reproducible and successful HILIC separations of polar analytes.

Part Number Short Description Phase Type Phase USP Particle Size ID Length Pore Size Type Price Qty
HILB-3-1003U ACE 3 HILIC-B 100 x 3mm Amino ACE HILIC-B L8 3 um 3.0 mm 100 mm 100 A Column
£947.43
HILB-3-1046U ACE 3 HILIC-B 100 x 4.6mm Amino ACE HILIC-B L8 3 um 4.6 mm 100 mm 100 A Column
£947.43
HILB-3-1546U ACE 3 HILIC-B 150 x 4.6mm Amino ACE HILIC-B L8 3 um 4.6 mm 150 mm 100 A Column
£1,021.71
HILB-3-2546U ACE 3 HILIC-B 250 x 4.6mm Amino ACE HILIC-B L8 3 um 4.6 mm 250 mm 100 A Column
£1,165.71
HILB-3-0302U ACE 3 HILIC-B 30 x 2.1mm Amino ACE HILIC-B L8 3 um 2.1 mm 30 mm 100 A Column
£802.29
HILB-3-0303U ACE 3 HILIC-B 30 x 3mm Amino ACE HILIC-B L8 3 um 3.0 mm 30 mm 100 A Column
£802.29
HILB-3-0502U ACE 3 HILIC-B 50 x 2.1mm Amino ACE HILIC-B L8 3 um 2.1 mm 50 mm 100 A Column
£848.00
HILB-3-0102GD ACE 3 HILIC-B Guards for 2.1mm ID Columns, 5pk Amino ACE HILIC-B L8 3 um 2.1 mm 100 A Guard
£265.14
HILB-3-0103GD ACE 3 HILIC-B Guards for 3.0 - 4.6mm ID Columns, 5pk Amino ACE HILIC-B L8 3 um 3.0 - 4.6 mm 100 A Guard
£290.29
HILB-5-1546U ACE 5 HILIC-B 150 x 4.6mm Amino ACE HILIC-B L8 5 um 4.6 mm 150 mm 100 A Column
£969.14
HILB-5-2546U ACE 5 HILIC-B 250 x 4.6mm Amino ACE HILIC-B L8 5 um 4.6 mm 250 mm 100 A Column
£1,021.71
HILB-5-0103GD ACE 5 HILIC-B Guards for 3.0 - 4.6mm ID Columns, 5pk Amino ACE HILIC-B L8 5 um 3.0 - 4.6 mm 100 A Guard
£290.29
  • HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionised analytes
  • ACE® HIILIC-B is a proprietary aminopropyl phase
  • Available in 1.7, 3 and 5 μm particle sizes
  • HPLC/UHPLC hardware format with 1,000 bar / 15,000 psi pressure limit
  • ACE® guard cartridges for analytical columns require stand-alone guard cartridge holder H0001 and column coupler C0001
  • ACE® method development kits offer all three Avantor® ACE® proprietary HILIC phases (HILIC-A, HILIC-B and HILIC-N) in one kit
  ACE® HILIC-B
Bonded Phase Proprietary Aminopropyl
Particle Type Fully Porous
Particle Size 1.7 µm, 3 µm, 5 µm
Pore Size 100 Å
Surface Area 300 m2/g
Endcapped No
Carbon Load 4%
pH Range 2 - 7
Pressure Limit 1000 bar
Hardware Stainless Steel
USP Designation L8
Avantor ACE HPLC and UHPLC Columns Catalogue (9.26 MB)
Product catalogue containing the full range of Avantor ACE columns
Avantor ACE Phase Guide (152.80 kB)
Specifications of the Avantor ACE columns
Avantor ACE HILIC Method Development Protocol (287.60 kB)
Step-by-step HILIC method development protocol
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