Avantor® ACE® HILIC-N HPLC Columns

Avantor® ACE® HILIC-N HPLC columns have been designed for HILIC applications and are suitable for the separation of polar compounds not retained by reversed phase methods. The HILIC-N stationary phase is a proprietary polyhydroxy with a polar neutral character capable of H-bonding amongst other mechanisms of interaction.

The ACE HILIC-N columns form part of a range of three proprietary HILIC phases (HILIC-A, HILIC-B, and HILIC-N) with alternative selectivity to each other. Using a screening approach to method development it is easy to achieve reproducible and successful HILIC separations of polar compounds.

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Part Number	Short Description	Phase Type	Phase	Particle Size	ID	Length	Pore Size	Type	Price
HILN-17-1002U	ACE 1.7 HILIC-N 100 x 2.1mm	Proprietary	ACE HILIC-N	1.7 um	2.1 mm	100 mm	100 A	Column	£1,128.00
HILN-17-0502U	ACE 1.7 HILIC-N 50 x 2.1mm	Proprietary	ACE HILIC-N	1.7 um	2.1 mm	50 mm	100 A	Column	£1,021.71
HILN-17-0503U	ACE 1.7 HILIC-N 50 x 3mm	Proprietary	ACE HILIC-N	1.7 um	3.0 mm	50 mm	100 A	Column	£1,021.71
HILN-17-7502U	ACE 1.7 HILIC-N 75 x 2.1mm	Proprietary	ACE HILIC-N	1.7 um	2.1 mm	75 mm	100 A	Column	£992.00
HILN-3-1002U	ACE 3 HILIC-N 100 x 2.1mm	Proprietary	ACE HILIC-N	3 um	2.1 mm	100 mm	100 A	Column	£947.43
HILN-3-1003U	ACE 3 HILIC-N 100 x 3mm	Proprietary	ACE HILIC-N	3 um	3.0 mm	100 mm	100 A	Column	£947.43
HILN-3-1046U	ACE 3 HILIC-N 100 x 4.6mm	Proprietary	ACE HILIC-N	3 um	4.6 mm	100 mm	100 A	Column	£867.43
HILN-3-1502U	ACE 3 HILIC-N 150 x 2.1mm	Proprietary	ACE HILIC-N	3 um	2.1 mm	150 mm	100 A	Column	£1,021.71
HILN-3-1503U	ACE 3 HILIC-N 150 x 3mm	Proprietary	ACE HILIC-N	3 um	3.0 mm	150 mm	100 A	Column	£1,021.71
HILN-3-1546U	ACE 3 HILIC-N 150 x 4.6mm	Proprietary	ACE HILIC-N	3 um	4.6 mm	150 mm	100 A	Column	£1,021.71
HILN-3-2546U	ACE 3 HILIC-N 250 x 4.6mm	Proprietary	ACE HILIC-N	3 um	4.6 mm	250 mm	100 A	Column	£1,165.71
HILN-3-0303U	ACE 3 HILIC-N 30 x 3mm	Proprietary	ACE HILIC-N	3 um	3.0 mm	30 mm	100 A	Column	£802.29
HILN-3-0502U	ACE 3 HILIC-N 50 x 2.1mm	Proprietary	ACE HILIC-N	3 um	2.1 mm	50 mm	100 A	Column	£776.00
HILN-3-0102GD	ACE 3 HILIC-N Guards for 2.1mm ID Columns, 5pk	Proprietary	ACE HILIC-N	3 um	2.1 mm		100 A	Guard	£265.14
HILN-3-0103GD	ACE 3 HILIC-N Guards for 3.0 - 4.6mm ID Columns, 5pk	Proprietary	ACE HILIC-N	3 um	3.0 - 4.6 mm		100 A	Guard	£265.14
HILN-5-1546U	ACE 5 HILIC-N 150 x 4.6mm	Proprietary	ACE HILIC-N	5 um	4.6 mm	150 mm	100 A	Column	£969.14
HILN-5-2503U	ACE 5 HILIC-N 250 x 3mm	Proprietary	ACE HILIC-N	5 um	3.0 mm	250 mm	100 A	Column	£1,021.71
HILN-5-0103GD	ACE 5 HILIC-N Guards for 3.0 - 4.6mm ID Columns, 5pk	Proprietary	ACE HILIC-N	5 um	3.0 - 4.6 mm		100 A	Guard	£265.14

Features

HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionised analytes
ACE® HILIC-N is a proprietary polyhydroxy phase
Available in 1.7, 3 and 5 μm particle sizes
HPLC/UHPLC hardware format with 1,000 bar / 15,000 psi pressure limit
ACE® guard cartridges for analytical columns require stand-alone guard cartridge holder H0001 and column coupler C0001
ACE® method development kits offer all three Avantor® ACE® proprietary HILIC phases (HILIC-A, HILIC-B and HILIC-N) in one kit

Specifications

	ACE® HILIC-N
Bonded Phase	Proprietary Polyhydroxy
Particle Type	Fully Porous
Particle Size	1.7 µm, 3 µm, 5 µm
Pore Size	100 Å
Surface Area	300 m²/g
Endcapped	No
Carbon Load	7%
pH Range	2 - 7
Pressure Limit	1000 bar
Hardware	Stainless Steel
USP Designation	Pending

Literature

Avantor ACE HPLC and UHPLC Columns Catalogue (9.26 MB)

Product catalogue containing the full range of Avantor ACE columns

Avantor ACE Phase Guide (152.80 kB)

Specifications of the Avantor ACE columns

Avantor ACE HILIC Method Development Protocol (287.60 kB)

Step-by-step HILIC method development protocol