Agilent ZORBAX HILIC Plus HPLC Columns

Agilent ZORBAX HILIC Plus HPLC columns are non-bonded silica columns made with ZORBAX Eclipse Plus silica optimised for retention and separation of small polar analytes. These columns are manufactured with traditional fully porous particles in 3.5 µm for HPLC separations or 1.8 µm for UHPLC applications. The robustness and reproducibility of HILIC separations on ZORBAX columns help to switch from challenging, often MS-incompatible ion pair chromatography and normal phase applications.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Buy

Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
821725-938	ZORBAX Eclipse HILIC 2.1mm 1.8um UHPLC Guard 3pk	Silica	ZORBAX HILIC Plus	L3	1.8 um	2.1 mm	5 mm	95 A	Guard	£468.00
823750-939	ZORBAX Eclipse HILIC 3mm 1.8um UHPLC Guard 3pk	Silica	ZORBAX HILIC Plus	L3	1.8 um	3.0 mm	5 mm	95 A	Guard	£468.00
959758-901	ZORBAX HILIC Plus 2.1 x 100mm 1.8um 1200 Bar RRHD Column	Silica	ZORBAX HILIC Plus	L3	1.8 um	2.1 mm	100 mm	95 A	Column	£643.00
959793-901	ZORBAX HILIC Plus 2.1 x 100mm 3.5um Column	Silica	ZORBAX HILIC Plus	L3	3.5 um	2.1 mm	100 mm	95 A	Column	£511.00
959759-901	ZORBAX HILIC Plus 2.1 x 150mm 1.8um 1200 Bar RRHD Column	Silica	ZORBAX HILIC Plus	L3	1.8 um	2.1 mm	150 mm	95 A	Column	£784.00
959757-901	ZORBAX HILIC Plus 2.1 x 50mm 1.8um 1200 Bar RRHD Column	Silica	ZORBAX HILIC Plus	L3	1.8 um	2.1 mm	50 mm	95 A	Column	£617.00
959743-901	ZORBAX HILIC Plus 2.1 x 50mm 3.5um Column	Silica	ZORBAX HILIC Plus	L3	3.5 um	2.1 mm	50 mm	95 A	Column	£394.00
959758-301	ZORBAX HILIC Plus 3 x 100mm 1.8um 1200 Bar RRHD Column	Silica	ZORBAX HILIC Plus	L3	1.8 um	3.0 mm	100 mm	95 A	Column	£643.00
959757-301	ZORBAX HILIC Plus 3 x 50mm 1.8um 1200 Bar RRHD Column	Silica	ZORBAX HILIC Plus	L3	1.8 um	3.0 mm	50 mm	95 A	Column	£617.00
959961-901	ZORBAX HILIC Plus 4.6 x 100mm 3.5um Column	Silica	ZORBAX HILIC Plus	L3	3.5 um	4.6 mm	100 mm	95 A	Column	£507.00
959943-901	ZORBAX HILIC Plus 4.6 x 50mm 3.5um Column	Silica	ZORBAX HILIC Plus	L3	3.5 um	4.6 mm	50 mm	95 A	Column	£390.00

Features

High-purity Type B silica for excellent peak shape especially for polar basic analytes
Traditional fully porous material in 1.8 µm and 3.5 µm
The high similarity of ZORBAX HILIC Plus and InfinityLab Poroshell 120 HILIC columns allows for easy method transfer to superficially porous column technology
Fully compatible with Agilent InfinityLab deactivator additive, which improves sensitivity in challenging analyses of phosphorylated and carboxylated compounds with stainless steel LC/MS systems
ZORBAX HILIC Plus is ideally suited for the retention of small, polar analytes and is recommended for EPA Method 1694
Columns ship containing acetonitrile/water and are ready to use for HILIC separations
1.8 µm UHPLC guard columns to further extend the life of your analytical column (no additional holder required)
USP L3 classification

Specifications

	ZORBAX HILIC Plus
Bonded Phase	Silica
Particle Type	Fully Porous
Particle Size	1.8 µm and 3.5 µm
Pore Size	95 Å
Surface Area	160 m²/g
Endcapped	No
Maximum Temperature	60°C
pH Range	0 - 8
Pressure Limit	3.5 µm - 400 bar 1.8 µm - 1200 bar
Hardware	Stainless Steel
USP Designation	L3

Literature

Zorbax Columns Poster (2.76 MB)

A proven and reliable portfolio of totally porous HPLC columns

HILIC Method Development Technical Overview (1.82 MB)

Hydrophilic interaction chromatography method development and troubleshooting