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SiELC Primesep 100 HPLC Columns

SiELC Primesep 100 is a mixed-mode HPLC column with acidic ion-pairing groups embedded into long hydrophobic ligand chains. The ligand itself is a C12 chain and its acidic functional group has a pKa of ~1. Reversed phase and cation-exchange functionality are assembled on one molecule to provide good reproducibility and stability.

Primesep 100 is the most versatile column in the entire Primesep family for separating a broad range of compounds. It enhances retention of basic compounds through cation-exchange mechanism, separates acids by ion-exclusion mechanism, and retains neutral compounds by reverse-phase mechanism. The columns are available in all standard dimensions, with 3 µm, 5 µm and 10 µm fully porous particles, and 2.7 µm solid core particles.

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Part Number Short Description Phase USP Particle Size ID Length Pore Size Type Price Qty
100-46.250.0310 Primesep 100 Column 4.6 x 250mm 3um 100A Primesep 100 L85 3 um 4.6 mm 250 mm 100 A Column
£1,415.13
100-46.250.1010 Primesep 100 Column 4.6 x 250mm 10um 100A Primesep 100 L85 10 um 4.6 mm 250 mm 100 A Column
£1,359.99
100-05.250.0510 Primesep 100 Column 0.5 x 250mm 5um 100A Primesep 100 L85 5 um 0.5 mm 250 mm 100 A Column
£1,409.00
100-05.150.0510 Primesep 100 Column 0.5 x 150mm 5um 100A Primesep 100 L85 5 um 0.5 mm 150 mm 100 A Column
£1,372.24
100-46.250.0510 Primesep 100 Column 4.6 x 250mm 5um 100A Primesep 100 L85 5 um 4.6 mm 250 mm 100 A Column
£1,280.36
100-05.100.0310 Primesep 100 Column 0.5 x 100mm 3um 100A Primesep 100 L85 3 um 0.5 mm 100 mm 100 A Column
£1,384.48
100-32.250.0310 Primesep 100 Column 3.2 x 250mm 3um 100A Primesep 100 L85 3 um 3.2 mm 250 mm 100 A Column
£1,359.99
100-46.150.0310 Primesep 100 Column 4.6 x 150mm 3um 100A Primesep 100 L85 3 um 4.6 mm 150 mm 100 A Column
£1,274.23
100-05.050.0310 Primesep 100 Column 0.5 x 50mm 3um 100A Primesep 100 L85 3 um 0.5 mm 50 mm 100 A Column
£1,372.24
100-05.050.0510 Primesep 100 Column 0.5 x 50mm 5um 100A Primesep 100 L85 5 um 0.5 mm 50 mm 100 A Column
£1,372.24
100-05.100.0510 Primesep 100 Column 0.5 x 100mm 5um 100A Primesep 100 L85 5 um 0.5 mm 100 mm 100 A Column
£1,384.48
100-05.250.0310 Primesep 100 Column 0.5 x 250mm 3um 100A Primesep 100 L85 3 um 0.5 mm 250 mm 100 A Column
£1,335.47
100-10.250.0310 Primesep 100 Column 1 x 250mm 3um 100A Primesep 100 L85 3 um 1.0 mm 250 mm 100 A Column
£1,323.24
100-21.250.0310 Primesep 100 Column 2.1 x 250mm 3um 100A Primesep 100 L85 3 um 2.1 mm 250 mm 100 A Column
£1,323.24
100-32.150.0310 Primesep 100 Column 3.2 x 150mm 3um 100A Primesep 100 L85 3 um 3.2 mm 150 mm 100 A Column
£1,286.46
100-10.150.0310 Primesep 100 Column 1 x 150mm 3um 100A Primesep 100 L85 3 um 1.0 mm 150 mm 100 A Column
£1,261.96
100-21.150.0310 Primesep 100 Column 2.1 x 150mm 3um 100A Primesep 100 L85 3 um 2.1 mm 150 mm 100 A Column
£1,261.96
100-46.150.0510 Primesep 100 Column 4.6 x 150mm 5um 100A Primesep 100 L85 5 um 4.6 mm 150 mm 100 A Column
£1,170.08
100-46.150.1010 Primesep 100 Column 4.6 x 150mm 10um 100A Primesep 100 L85 10 um 4.6 mm 150 mm 100 A Column
£1,170.08
100-32.250.0510 Primesep 100 Column 3.2 x 250mm 5um 100A Primesep 100 L85 5 um 3.2 mm 250 mm 100 A Column
£1,237.47
100-10.100.0310 Primesep 100 Column 1 x 100mm 3um 100A Primesep 100 L85 3 um 1.0 mm 100 mm 100 A Column
£1,225.22
100-10.250.0510 Primesep 100 Column 1 x 250mm 5um 100A Primesep 100 L85 5 um 1.0 mm 250 mm 100 A Column
£1,200.72
100-21.100.0310 Primesep 100 Column 2.1 x 100mm 3um 100A Primesep 100 L85 3 um 2.1 mm 100 mm 100 A Column
£1,225.22
100-21.250.0510 Primesep 100 Column 2.1 x 250mm 5um 100A Primesep 100 L85 5 um 2.1 mm 250 mm 100 A Column
£1,200.72
100-32.100.0310 Primesep 100 Column 3.2 x 100mm 3um 100A Primesep 100 L85 3 um 3.2 mm 100 mm 100 A Column
£1,225.22
100-46.100.0310 Primesep 100 Column 4.6 x 100mm 3um 100A Primesep 100 L85 3 um 4.6 mm 100 mm 100 A Column
£1,151.71
100-32.250.1010 Primesep 100 Column 3.2 x 250mm 10um 100A Primesep 100 L85 10 um 3.2 mm 250 mm 100 A Column
£1,188.46
100-46.100.0510 Primesep 100 Column 4.6 x 100mm 5um 100A Primesep 100 L85 5 um 4.6 mm 100 mm 100 A Column
£1,133.34
100-46.100.1010 Primesep 100 Column 4.6 x 100mm 10um 100A Primesep 100 L85 10 um 4.6 mm 100 mm 100 A Column
£1,133.34
100-32.150.0510 Primesep 100 Column 3.2 x 150mm 5um 100A Primesep 100 L85 5 um 3.2 mm 150 mm 100 A Column
£1,176.20
100-21.050.0310 Primesep 100 Column 2.1 x 50mm 3um 100A Primesep 100 L85 3 um 2.1 mm 50 mm 100 A Column
£1,200.72
100-32.050.0310 Primesep 100 Column 3.2 x 50mm 3um 100A Primesep 100 L85 3 um 3.2 mm 50 mm 100 A Column
£1,200.72
100-46.050.0310 Primesep 100 Column 4.6 x 50mm 3um 100A Primesep 100 L85 3 um 4.6 mm 50 mm 100 A Column
£1,133.34
100-10.150.0510 Primesep 100 Column 1 x 150mm 5um 100A Primesep 100 L85 5 um 1.0 mm 150 mm 100 A Column
£1,151.71
100-21.150.0510 Primesep 100 Column 2.1 x 150mm 5um 100A Primesep 100 L85 5 um 2.1 mm 150 mm 100 A Column
£1,151.71
100-10.050.0310 Primesep 100 Column 1 x 50mm 3um 100A Primesep 100 L85 3 um 1.0 mm 50 mm 100 A Column
£1,188.46
100-32.100.0510 Primesep 100 Column 3.2 x 100mm 5um 100A Primesep 100 L85 5 um 3.2 mm 100 mm 100 A Column
£1,149.26
100-32.150.1010 Primesep 100 Column 3.2 x 150mm 10um 100A Primesep 100 L85 10 um 3.2 mm 150 mm 100 A Column
£1,133.34
100-10.100.0510 Primesep 100 Column 1 x 100mm 5um 100A Primesep 100 L85 5 um 1.0 mm 100 mm 100 A Column
£1,133.34
100-21.100.0510 Primesep 100 Column 2.1 x 100mm 5um 100A Primesep 100 L85 5 um 2.1 mm 100 mm 100 A Column
£1,133.34
100-32.100.1010 Primesep 100 Column 3.2 x 100mm 10um 100A Primesep 100 L85 10 um 3.2 mm 100 mm 100 A Column
£1,102.70
100-10.050.0510 Primesep 100 Column 1 x 50mm 5um 100A Primesep 100 L85 5 um 1.0 mm 50 mm 100 A Column
£1,096.57
100-21.050.0510 Primesep 100 Column 2.1 x 50mm 5um 100A Primesep 100 L85 5 um 2.1 mm 50 mm 100 A Column
£1,096.57
100-32.050.0510 Primesep 100 Column 3.2 x 50mm 5um 100A Primesep 100 L85 5 um 3.2 mm 50 mm 100 A Column
£1,096.57
100-46.050.0510 Primesep 100 Column 4.6 x 50mm 5um 100A Primesep 100 L85 5 um 4.6 mm 50 mm 100 A Column
£1,029.19
100-46.050.1010 Primesep 100 Column 4.6 x 50mm 10um 100A Primesep 100 L85 10 um 4.6 mm 50 mm 100 A Column
£1,029.19
100-32.025.1010 Primesep 100 Column 3.2 x 25mm 10um 100A Primesep 100 L85 10 um 3.2 mm 25 mm 100 A Column
£1,057.36
100-32.050.1010 Primesep 100 Column 3.2 x 50mm 10um 100A Primesep 100 L85 10 um 3.2 mm 50 mm 100 A Column
£1,037.14
100-10.025.0310 Primesep 100 Column 1 x 25mm 3um 100A Primesep 100 L85 3 um 1.0 mm 25 mm 100 A Column
£980.18
100-10.025.0510 Primesep 100 Column 1 x 25mm 5um 100A Primesep 100 L85 5 um 1.0 mm 25 mm 100 A Column
£980.18
100-21.025.0310 Primesep 100 Column 2.1 x 25mm 3um 100A Primesep 100 L85 3 um 2.1 mm 25 mm 100 A Column
£980.18
100-21.025.0510 Primesep 100 Column 2.1 x 25mm 5um 100A Primesep 100 L85 5 um 2.1 mm 25 mm 100 A Column
£980.18
100-32.025.0310 Primesep 100 Column 3.2 x 25mm 3um 100A Primesep 100 L85 3 um 3.2 mm 25 mm 100 A Column
£980.18
100-32.025.0510 Primesep 100 Column 3.2 x 25mm 5um 100A Primesep 100 L85 5 um 3.2 mm 25 mm 100 A Column
£980.18
100-10.010.0310 Primesep 100 Column 1 x 10mm 3um 100A Primesep 100 L85 3 um 1.0 mm 10 mm 100 A Column
£888.29
100-10.010.0510 Primesep 100 Column 1 x 10mm 5um 100A Primesep 100 L85 5 um 1.0 mm 10 mm 100 A Column
£888.29
100-21.010.0310 Primesep 100 Column 2.1 x 10mm 3um 100A Primesep 100 L85 3 um 2.1 mm 10 mm 100 A Column
£888.29
100-21.010.0510 Primesep 100 Column 2.1 x 10mm 5um 100A Primesep 100 L85 5 um 2.1 mm 10 mm 100 A Column
£888.29
100-32.010.0310 Primesep 100 Column 3.2 x 10mm 3um 100A Primesep 100 L85 3 um 3.2 mm 10 mm 100 A Column
£888.29
100-32.010.0510 Primesep 100 Column 3.2 x 10mm 5um 100A Primesep 100 L85 5 um 3.2 mm 10 mm 100 A Column
£888.29
100-46.010.0310 Primesep 100 Column 4.6 x 10mm 3um 100A Primesep 100 L85 3 um 4.6 mm 10 mm 100 A Column
£829.52
100-46.010.0510 Primesep 100 Column 4.6 x 10mm 5um 100A Primesep 100 L85 5 um 4.6 mm 10 mm 100 A Column
£829.52
100-10.G.0310 Primesep 100 Guard Column 1 x 10mm 3um 100A, 2pk Primesep 100 L85 3 um 1.0 mm 10 mm 100 A Guard
£441.08
100-21.G.0310 Primesep 100 Guard Column 2.1 x 10mm 3um 100A, 2pk Primesep 100 L85 3 um 2.1 mm 10 mm 100 A Guard
£441.08
100-32.G.0310 Primesep 100 Guard Column 3.2 x 10mm 3um 100A, 2pk Primesep 100 L85 3 um 3.2 mm 10 mm 100 A Guard
£441.08
100-46.G.0310 Primesep 100 Guard Column 4.6 x 10mm 3um 100A, 2pk Primesep 100 L85 3 um 4.6 mm 10 mm 100 A Guard
£441.08
100-10.G.0510 Primesep 100 Guard Column 1 x 10mm 5um 100A, 2pk Primesep 100 L85 5 um 1.0 mm 10 mm 100 A Guard
£416.57
100-21.G.0510 Primesep 100 Guard Column 2.1 x 10mm 5um 100A, 2pk Primesep 100 L85 5 um 2.1 mm 10 mm 100 A Guard
£416.57
100-32.G.0510 Primesep 100 Guard Column 3.2 x 10mm 5um 100A, 2pk Primesep 100 L85 5 um 3.2 mm 10 mm 100 A Guard
£416.57
100-46.G.0510 Primesep 100 Guard Column 4.6 x 10mm 5um 100A, 2pk Primesep 100 L85 5 um 4.6 mm 10 mm 100 A Guard
£416.57
100-100.050.0510 Primesep 100 Column 10 x 50mm 5um 100A Primesep 100 L85 5 um 10.0 mm 50 mm 100 A Column
£2,839.72
100-100.050.1010 Primesep 100 Column 10 x 50mm 10um 100A Primesep 100 L85 10 um 10.0 mm 50 mm 100 A Column
£2,638.07
100-100.100.0510 Primesep 100 Column 10 x 100mm 5um 100A Primesep 100 L85 5 um 10.0 mm 100 mm 100 A Column
£3,394.20
100-100.100.1010 Primesep 100 Column 10 x 100mm 10um 100A Primesep 100 L85 10 um 10.0 mm 100 mm 100 A Column
£3,209.37
100-100.150.0510 Primesep 100 Column 10 x 150mm 5um 100A Primesep 100 L85 5 um 10.0 mm 150 mm 100 A Column
£3,965.51
100-100.150.1010 Primesep 100 Column 10 x 150mm 10um 100A Primesep 100 L85 10 um 10.0 mm 150 mm 100 A Column
£3,780.69
100-100.250.0510 Primesep 100 Column 10 x 250mm 5um 100A Primesep 100 L85 5 um 10.0 mm 250 mm 100 A Column
£5,192.15
100-100.250.1010 Primesep 100 Column 10 x 250mm 10um 100A Primesep 100 L85 10 um 10.0 mm 250 mm 100 A Column
£4,721.64
100-220.050.0510 Primesep 100 Column 22 x 50mm 5um 100A Primesep 100 L85 5 um 22.0 mm 50 mm 100 A Column
£5,091.33
100-220.050.1010 Primesep 100 Column 22 x 50mm 10um 100A Primesep 100 L85 10 um 22.0 mm 50 mm 100 A Column
£4,822.46
100-220.100.0510 Primesep 100 Column 22 x 100mm 5um 100A Primesep 100 L85 5 um 22.0 mm 100 mm 100 A Column
£5,847.47
100-220.100.1010 Primesep 100 Column 22 x 100mm 10um 100A Primesep 100 L85 10 um 22.0 mm 100 mm 100 A Column
£5,477.79
100-220.150.0510 Primesep 100 Column 22 x 150mm 5um 100A Primesep 100 L85 5 um 22.0 mm 150 mm 100 A Column
£7,275.73
100-220.150.1010 Primesep 100 Column 22 x 150mm 10um 100A Primesep 100 L85 10 um 22.0 mm 150 mm 100 A Column
£6,889.25
100-220.250.0510 Primesep 100 Column 22 x 250mm 5um 100A Primesep 100 L85 5 um 22.0 mm 250 mm 100 A Column
£9,157.67
100-220.250.1010 Primesep 100 Column 22 x 250mm 10um 100A Primesep 100 L85 10 um 22.0 mm 250 mm 100 A Column
£8,788.00
100-32.100.0209 Primesep 100 Column 3.2 x 100mm 2.7um 90A Primesep 100 L85 2.7 um 3.2 mm 100 mm 90 A Column
£1,249.72
100-32.050.0209 Primesep 100 Column 3.2 x 50mm 2.7um 90A Primesep 100 L85 2.7 um 3.2 mm 50 mm 90 A Column
£1,249.72
100-32.025.0209 Primesep 100 Column 3.2 x 25mm 2.7um 90A Primesep 100 L85 2.7 um 3.2 mm 25 mm 90 A Column
£1,249.72
100-32.010.0209 Primesep 100 Column 3.2 x 10mm 2.7um 90A Primesep 100 L85 2.7 um 3.2 mm 10 mm 90 A Column
£1,249.72
100-32.G.0209 Primesep 100 Guard Column 3.2 x 10mm 2.7um 90A, 2pk Primesep 100 L85 2.7 um 3.2 mm 10 mm 90 A Guard
£551.34
100-21.100.0209 Primesep 100 Column 2.1 x 100mm 2.7um 90A Primesep 100 L85 2.7 um 2.1 mm 100 mm 90 A Column
£1,200.72
100-21.050.0209 Primesep 100 Column 2.1 x 50mm 2.7um 90A Primesep 100 L85 2.7 um 2.1 mm 50 mm 90 A Column
£1,200.72
100-21.025.0209 Primesep 100 Column 2.1 x 25mm 2.7um 90A Primesep 100 L85 2.7 um 2.1 mm 25 mm 90 A Column
£1,200.72
100-21.010.0209 Primesep 100 Column 2.1 x 10mm 2.7um 90A Primesep 100 L85 2.7 um 2.1 mm 10 mm 90 A Column
£1,200.72
100-21.G.0209 Primesep 100 Guard Column 2.1 x 10mm 2.7um 90A, 2pk Primesep 100 L85 2.7 um 2.1 mm 10 mm 90 A Guard
£526.83
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Features
  • Primesep 100 is a mixed-mode column with acidic ion-pairing groups embedded into long hydrophobic ligand chains
  • Available with 3 µm, 5 µm, and 10 µm fully porous particles as well as 2.7 µm solid-core particles
  • Most versatile in the entire Primesep family for separating a broad range of compounds
  • Enhanced retention of basic compounds through cation-exchange mechanism
  • Separates acids by ion-exclusion mechanism
  • Retains neutral compounds by reverse-phase mechanism
  • Typical mobile phases are based on acetonitrile, water, and an acidic buffer
  • Offers mobile phase compatibility with LC-MS
  • Resistant to dewetting in 100% aqueous mobile phase
  • Scalable from capillary to prep
  • Guard columns connect directly to columns and do not require any additional holders
Specifications
  Primesep 100
Mode Mixed-Mode
Phase Type Acidic ion-pairing groups embedded into long hydrophobic ligand chains
Particle Type Solid Core (2.7 µm) and Fully Porous (3 µm, 5 µm, 10 µm)
Particle Size 2.7 µm, 3 µm, 5 µm, 10 µm
Pore Size 90 Å (2.7 µm) and 100 Å (3 µm, 5 µm, 10 µm)
Temperature Range 0 - 50°C
pH Limit 1.5 - 7.0
Recommended pH Operating Range 2.0 - 6.5
Pressure Limit 345 bar
Literature
SiELC Primesep 100 Brochure (1.02 MB)
Methods and applications using Primesep 100 columns
SiELC Primesep Brochure (1.10 MB)
Creating new dimensions in the world of chromatography
Basic Dyes Application Note using BIST B+ and Primesep 100 Columns (1.00 MB)
Multi charged basic dye mixtures separated by BIST B+ and singly charged dyes separated by Primesep 100
SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)
Solving problems of pharmaceutical HPLC analysis using Primesep columns
SiELC Primesep - Separation of Ions Brochure (293.32 kB)
New alternative to ion chromatography
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