SiELC Primesep 500 HPLC Columns

SiELC Primesep 500 is a mixed-mode HPLC column with acidic ion-pairing groups embedded into long ligand chains. The embedded group is a carboxylic acid with a pKa of about 5. Primesep 500 provides enhanced retention of basic compounds through a cation-exchange mechanism with a mobile phase of pH > 5, separates acids by an ion-exclusion mechanism with a mobile phase of pH > 5, retains basic compounds at pH < 5, and retains neutral compounds by a reversed phase mechanism.

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Part Number	Short Description	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
500-05.250.0510	Primesep 500 Column 0.5 x 250mm 5um 100A	Primesep 500	L85	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
500-05.150.0510	Primesep 500 Column 0.5 x 150mm 5um 100A	Primesep 500	L85	5 um	0.5 mm	150 mm	100 A	Column	£1,396.75
500-46.250.0510	Primesep 500 Column 4.6 x 250mm 5um 100A	Primesep 500	L85	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
500-05.100.0510	Primesep 500 Column 0.5 x 100mm 5um 100A	Primesep 500	L85	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
500-46.150.0510	Primesep 500 Column 4.6 x 150mm 5um 100A	Primesep 500	L85	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
500-32.250.0510	Primesep 500 Column 3.2 x 250mm 5um 100A	Primesep 500	L85	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
500-10.250.0510	Primesep 500 Column 1 x 250mm 5um 100A	Primesep 500	L85	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
500-21.250.0510	Primesep 500 Column 2.1 x 250mm 5um 100A	Primesep 500	L85	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
500-46.100.0510	Primesep 500 Column 4.6 x 100mm 5um 100A	Primesep 500	L85	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
500-32.150.0510	Primesep 500 Column 3.2 x 150mm 5um 100A	Primesep 500	L85	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
500-10.150.0510	Primesep 500 Column 1 x 150mm 5um 100A	Primesep 500	L85	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
500-21.150.0510	Primesep 500 Column 2.1 x 150mm 5um 100A	Primesep 500	L85	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
500-32.100.0510	Primesep 500 Column 3.2 x 100mm 5um 100A	Primesep 500	L85	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
500-10.100.0510	Primesep 500 Column 1 x 100mm 5um 100A	Primesep 500	L85	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
500-21.100.0510	Primesep 500 Column 2.1 x 100mm 5um 100A	Primesep 500	L85	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
500-10.050.0510	Primesep 500 Column 1 x 50mm 5um 100A	Primesep 500	L85	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
500-21.050.0510	Primesep 500 Column 2.1 x 50mm 5um 100A	Primesep 500	L85	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
500-32.050.0510	Primesep 500 Column 3.2 x 50mm 5um 100A	Primesep 500	L85	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
500-46.050.0510	Primesep 500 Column 4.6 x 50mm 5um 100A	Primesep 500	L85	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
500-10.025.0510	Primesep 500 Column 1 x 25mm 5um 100A	Primesep 500	L85	5 um	1.0 mm	25 mm	100 A	Column	£980.18
500-21.025.0510	Primesep 500 Column 2.1 x 25mm 5um 100A	Primesep 500	L85	5 um	2.1 mm	25 mm	100 A	Column	£980.18
500-32.025.0510	Primesep 500 Column 3.2 x 25mm 5um 100A	Primesep 500	L85	5 um	3.2 mm	25 mm	100 A	Column	£980.18
500-10.010.0510	Primesep 500 Column 1 x 10mm 5um 100A	Primesep 500	L85	5 um	1.0 mm	10 mm	100 A	Column	£888.29
500-21.010.0510	Primesep 500 Column 2.1 x 10mm 5um 100A	Primesep 500	L85	5 um	2.1 mm	10 mm	100 A	Column	£888.29
500-32.010.0510	Primesep 500 Column 3.2 x 10mm 5um 100A	Primesep 500	L85	5 um	3.2 mm	10 mm	100 A	Column	£888.29
500-46.010.0510	Primesep 500 Column 4.6 x 10mm 5um 100A	Primesep 500	L85	5 um	4.6 mm	10 mm	100 A	Column	£829.49
500-10.G.0510	Primesep 500 Guard Column 1 x 10mm 5um 100A, 2pk	Primesep 500	L85	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
500-21.G.0510	Primesep 500 Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep 500	L85	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
500-32.G.0510	Primesep 500 Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep 500	L85	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
500-46.G.0510	Primesep 500 Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep 500	L85	5 um	4.6 mm	10 mm	100 A	Guard	£392.07
500-100.050.0510	Primesep 500 Column 10 x 50mm 5um 100A	Primesep 500	L85	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
500-100.100.0510	Primesep 500 Column 10 x 100mm 5um 100A	Primesep 500	L85	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
500-100.150.0510	Primesep 500 Column 10 x 150mm 5um 100A	Primesep 500	L85	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
500-100.250.0510	Primesep 500 Column 10 x 250mm 5um 100A	Primesep 500	L85	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
500-220.050.0510	Primesep 500 Column 22 x 50mm 5um 100A	Primesep 500	L85	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
500-220.100.0510	Primesep 500 Column 22 x 100mm 5um 100A	Primesep 500	L85	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
500-220.150.0510	Primesep 500 Column 22 x 150mm 5um 100A	Primesep 500	L85	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
500-220.250.0510	Primesep 500 Column 22 x 250mm 5um 100A	Primesep 500	L85	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67

Features

Primesep 500 mixed-mode column with carboxylic acid groups (pKa ~ 5) embedded into long ligand chains
Enhances retention of basic compounds through a cation-exchange mechanism with a mobile phase of pH > 5
Separates acids by an ion-exclusion mechanism with a mobile phase of pH > 5
Retains basic compounds, like any reverse-phase column, at pH < 5
Retains neutral compounds by reverse-phase mechanism
Typical mobile phases are based on acetonitrile, water, and an acidic buffer
Offers mobile phase compatibility with LC-MS
Resistant to dewetting in 100% aqueous mobile phase
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep 500
Mode	Mixed-Mode
Phase Type	Acidic ion-pairing groups embedded into long ligand chains
Particle Type	Fully Porous
Particle Size	5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography