SiELC Primesep A HPLC Columns

SiELC Primesep A is a mixed-mode HPLC column with strong acidic ion-pairing groups embedded in long ligand chains. It provides enhanced retention of weakly basic compounds through a cation exchange mechanism, separates acids by ion-exclusion, and retains neutral compounds by reversed phase mode.

The method development process using Primesep A is simple and versatile. In most cases, separations require a simple mobile phase containing acetonitrile, water and TFA or formic acid. No ion-pairing reagents are required for the retention of ionizable polar analytes. The simple mobile phases allow compatibility with all common detectors (UV, MS, ELSD, and RI).

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
A-05.250.0510	Primesep A Column 0.5 x 250mm 5um 100A	Primesep A	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
A-05.150.0510	Primesep A Column 0.5 x 150mm 5um 100A	Primesep A	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
A-46.250.0510	Primesep A Column 4.6 x 250mm 5um 100A	Primesep A	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
A-05.100.0510	Primesep A Column 0.5 x 100mm 5um 100A	Primesep A	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
A-46.150.0510	Primesep A Column 4.6 x 150mm 5um 100A	Primesep A	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
A-32.250.0510	Primesep A Column 3.2 x 250mm 5um 100A	Primesep A	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
A-10.250.0510	Primesep A Column 1 x 250mm 5um 100A	Primesep A	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
A-21.250.0510	Primesep A Column 2.1 x 250mm 5um 100A	Primesep A	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
A-46.100.0510	Primesep A Column 4.6 x 100mm 5um 100A	Primesep A	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
A-32.150.0510	Primesep A Column 3.2 x 150mm 5um 100A	Primesep A	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
A-10.150.0510	Primesep A Column 1 x 150mm 5um 100A	Primesep A	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
A-21.150.0510	Primesep A Column 2.1 x 150mm 5um 100A	Primesep A	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
A-32.100.0510	Primesep A Column 3.2 x 100mm 5um 100A	Primesep A	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
A-10.100.0510	Primesep A Column 1 x 100mm 5um 100A	Primesep A	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
A-21.100.0510	Primesep A Column 2.1 x 100mm 5um 100A	Primesep A	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
A-10.050.0510	Primesep A Column 1 x 50mm 5um 100A	Primesep A	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
A-21.050.0510	Primesep A Column 2.1 x 50mm 5um 100A	Primesep A	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
A-32.050.0510	Primesep A Column 3.2 x 50mm 5um 100A	Primesep A	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
A-46.050.0510	Primesep A Column 4.6 x 50mm 5um 100A	Primesep A	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
A-10.025.0510	Primesep A Column 1 x 25mm 5um 100A	Primesep A	5 um	1.0 mm	25 mm	100 A	Column	£980.18
A-21.025.0510	Primesep A Column 2.1 x 25mm 5um 100A	Primesep A	5 um	2.1 mm	25 mm	100 A	Column	£980.18
A-32.025.0510	Primesep A Column 3.2 x 25mm 5um 100A	Primesep A	5 um	3.2 mm	25 mm	100 A	Column	£980.18
A-10.010.0510	Primesep A Column 1 x 10mm 5um 100A	Primesep A	5 um	1.0 mm	10 mm	100 A	Column	£888.29
A-21.010.0510	Primesep A Column 2.1 x 10mm 5um 100A	Primesep A	5 um	2.1 mm	10 mm	100 A	Column	£888.29
A-32.010.0510	Primesep A Column 3.2 x 10mm 5um 100A	Primesep A	5 um	3.2 mm	10 mm	100 A	Column	£888.29
A-46.010.0510	Primesep A Column 4.6 x 10mm 5um 100A	Primesep A	5 um	4.6 mm	10 mm	100 A	Column	£829.49
A-10.G.0510	Primesep A Guard Column 1 x 10mm 5um 100A, 2pk	Primesep A	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
A-21.G.0510	Primesep A Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep A	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
A-32.G.0510	Primesep A Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep A	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
A-46.G.0510	Primesep A Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep A	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
A-100.050.0510	Primesep A Column 10 x 50mm 5um 100A	Primesep A	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
A-100.100.0510	Primesep A Column 10 x 100mm 5um 100A	Primesep A	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
A-100.150.0510	Primesep A Column 10 x 150mm 5um 100A	Primesep A	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
A-100.250.0510	Primesep A Column 10 x 250mm 5um 100A	Primesep A	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
A-220.050.0510	Primesep A Column 22 x 50mm 5um 100A	Primesep A	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
A-220.100.0510	Primesep A Column 22 x 100mm 5um 100A	Primesep A	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
A-220.150.0510	Primesep A Column 22 x 150mm 5um 100A	Primesep A	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
A-220.250.0510	Primesep A Column 22 x 250mm 5um 100A	Primesep A	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67

Features

Primesep A stationary phase has strong acidic ion-pairing groups embedded in long ligand chains
Enhances retention of weak basic compounds through cation-exchange mechanism
Separates acids using ion-exclusion mechanism
Retains neutral compounds by reversed phase mechanism
Offers mobile phase compatibility with LC-MS
Resistant to dewetting in 100% aqueous mobile phase
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep A
Mode	Mixed-Mode
Phase Type	Strong acidic ion-pairing groups embedded in long ligand chains
Particle Type	Fully Porous
Particle Size	5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
pH Limit	1.5 - 7.0
Recommended pH Operating Range	2.0 - 6.5
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography