SiELC primesep B HPLC Columns

Primesep B from SiELC is a mixed-mode HPLC column with basic ion-pairing groups with a pKa value of 10 embedded on C12 carbon chains. The recommended pH range is from 1.5 to 4, created by the addition of trifluoroacetic, phosphoric, perchloric, or formic acids to the mobile phase. Primesep B effectively retains both hydrophobic and acidic components using simple mobile phase systems.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
B-05.250.0510	Primesep B Column 0.5 x 250mm 5um 100A	Primesep B	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
B-05.150.0510	Primesep B Column 0.5 x 150mm 5um 100A	Primesep B	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
B-46.250.0510	Primesep B Column 4.6 x 250mm 5um 100A	Primesep B	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
B-05.100.0510	Primesep B Column 0.5 x 100mm 5um 100A	Primesep B	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
B-46.150.0510	Primesep B Column 4.6 x 150mm 5um 100A	Primesep B	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
B-32.250.0510	Primesep B Column 3.2 x 250mm 5um 100A	Primesep B	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
B-10.250.0510	Primesep B Column 1 x 250mm 5um 100A	Primesep B	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
B-21.250.0510	Primesep B Column 2.1 x 250mm 5um 100A	Primesep B	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
B-46.100.0510	Primesep B Column 4.6 x 100mm 5um 100A	Primesep B	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
B-32.150.0510	Primesep B Column 3.2 x 150mm 5um 100A	Primesep B	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
B-10.150.0510	Primesep B Column 1 x 150mm 5um 100A	Primesep B	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
B-21.150.0510	Primesep B Column 2.1 x 150mm 5um 100A	Primesep B	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
B-32.100.0510	Primesep B Column 3.2 x 100mm 5um 100A	Primesep B	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
B-10.100.0510	Primesep B Column 1 x 100mm 5um 100A	Primesep B	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
B-21.100.0510	Primesep B Column 2.1 x 100mm 5um 100A	Primesep B	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
B-10.050.0510	Primesep B Column 1 x 50mm 5um 100A	Primesep B	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
B-21.050.0510	Primesep B Column 2.1 x 50mm 5um 100A	Primesep B	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
B-32.050.0510	Primesep B Column 3.2 x 50mm 5um 100A	Primesep B	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
B-46.050.0510	Primesep B Column 4.6 x 50mm 5um 100A	Primesep B	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
B-10.025.0510	Primesep B Column 1 x 25mm 5um 100A	Primesep B	5 um	1.0 mm	25 mm	100 A	Column	£980.18
B-21.025.0510	Primesep B Column 2.1 x 25mm 5um 100A	Primesep B	5 um	2.1 mm	25 mm	100 A	Column	£980.18
B-32.025.0510	Primesep B Column 3.2 x 25mm 5um 100A	Primesep B	5 um	3.2 mm	25 mm	100 A	Column	£980.18
B-10.010.0510	Primesep B Column 1 x 10mm 5um 100A	Primesep B	5 um	1.0 mm	10 mm	100 A	Column	£888.29
B-21.010.0510	Primesep B Column 2.1 x 10mm 5um 100A	Primesep B	5 um	2.1 mm	10 mm	100 A	Column	£888.29
B-32.010.0510	Primesep B Column 3.2 x 10mm 5um 100A	Primesep B	5 um	3.2 mm	10 mm	100 A	Column	£888.29
B-46.010.0510	Primesep B Column 4.6 x 10mm 5um 100A	Primesep B	5 um	4.6 mm	10 mm	100 A	Column	£829.49
B-10.G.0510	Primesep B Guard Column 1 x 10mm 5um 100A, 2pk	Primesep B	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
B-21.G.0510	Primesep B Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep B	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
B-32.G.0510	Primesep B Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep B	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
B-46.G.0510	Primesep B Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep B	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
B-100.050.0510	Primesep B Column 10 x 50mm 5um 100A	Primesep B	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
B-100.100.0510	Primesep B Column 10 x 100mm 5um 100A	Primesep B	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
B-100.150.0510	Primesep B Column 10 x 150mm 5um 100A	Primesep B	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
B-100.250.0510	Primesep B Column 10 x 250mm 5um 100A	Primesep B	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
B-220.050.0510	Primesep B Column 22 x 50mm 5um 100A	Primesep B	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
B-220.100.0510	Primesep B Column 22 x 100mm 5um 100A	Primesep B	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
B-220.150.0510	Primesep B Column 22 x 150mm 5um 100A	Primesep B	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
B-220.250.0510	Primesep B Column 22 x 250mm 5um 100A	Primesep B	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67
B-05.050.0310	Primesep B Column 0.5 x 50mm 3um 100A	Primesep B	3 um	0.5 mm	50 mm	100 A	Column	£1,372.24
B-05.050.0510	Primesep B Column 0.5 x 50mm 5um 100A	Primesep B	5 um	0.5 mm	50 mm	100 A	Column	£1,372.24
B-05.100.0310	Primesep B Column 0.5 x 100mm 3um 100A	Primesep B	3 um	0.5 mm	100 mm	100 A	Column	£1,384.48
B-05.250.0310	Primesep B Column 0.5 x 250mm 3um 100A	Primesep B	3 um	0.5 mm	250 mm	100 A	Column	£1,335.47
B-10.010.0310	Primesep B Column 1 x 10mm 3um 100A	Primesep B	3 um	1.0 mm	10 mm	100 A	Column	£888.29
B-10.025.0310	Primesep B Column 1 x 25mm 3um 100A	Primesep B	3 um	1.0 mm	25 mm	100 A	Column	£980.18
B-10.050.0310	Primesep B Column 1 x 50mm 3um 100A	Primesep B	3 um	1.0 mm	50 mm	100 A	Column	£1,188.46
B-10.100.0310	Primesep B Column 1 x 100mm 3um 100A	Primesep B	3 um	1.0 mm	100 mm	100 A	Column	£1,102.70
B-10.150.0310	Primesep B Column 1 x 150mm 3um 100A	Primesep B	3 um	1.0 mm	150 mm	100 A	Column	£1,261.96
B-10.250.0310	Primesep B Column 1 x 250mm 3um 100A	Primesep B	3 um	1.0 mm	250 mm	100 A	Column	£1,323.24
B-10.G.0310	Primesep B Guard Column 1 x 10mm 3um 100A, 2pk	Primesep B	3 um	1.0 mm	10 mm	100 A	Guard	£441.08
B-21.010.0310	Primesep B Column 2.1 x 10mm 3um 100A	Primesep B	3 um	2.1 mm	10 mm	100 A	Column	£888.29
B-21.025.0310	Primesep B Column 2.1 x 25mm 3um 100A	Primesep B	3 um	2.1 mm	25 mm	100 A	Column	£980.18
B-21.050.0310	Primesep B Column 2.1 x 50mm 3um 100A	Primesep B	3 um	2.1 mm	50 mm	100 A	Column	£1,200.72
B-21.100.0310	Primesep B Column 2.1 x 100mm 3um 100A	Primesep B	3 um	2.1 mm	100 mm	100 A	Column	£1,225.22
B-21.150.0310	Primesep B Column 2.1 x 150mm 3um 100A	Primesep B	3 um	2.1 mm	150 mm	100 A	Column	£1,261.96
B-21.250.0310	Primesep B Column 2.1 x 250mm 3um 100A	Primesep B	3 um	2.1 mm	250 mm	100 A	Column	£1,323.24
B-21.G.0310	Primesep B Guard Column 2.1 x 10mm 3um 100A, 2pk	Primesep B	3 um	2.1 mm	10 mm	100 A	Guard	£441.08
B-32.010.0310	Primesep B Column 3.2 x 10mm 3um 100A	Primesep B	3 um	3.2 mm	10 mm	100 A	Column	£888.29
B-32.025.0310	Primesep B Column 3.2 x 25mm 3um 100A	Primesep B	3 um	3.2 mm	25 mm	100 A	Column	£980.18
B-32.050.0310	Primesep B Column 3.2 x 50mm 3um 100A	Primesep B	3 um	3.2 mm	50 mm	100 A	Column	£1,200.72
B-32.100.0310	Primesep B Column 3.2 x 100mm 3um 100A	Primesep B	3 um	3.2 mm	100 mm	100 A	Column	£1,225.22
B-32.150.0310	Primesep B Column 3.2 x 150mm 3um 100A	Primesep B	3 um	3.2 mm	150 mm	100 A	Column	£1,286.46
B-32.250.0310	Primesep B Column 3.2 x 250mm 3um 100A	Primesep B	3 um	3.2 mm	250 mm	100 A	Column	£1,359.99
B-32.G.0310	Primesep B Guard Column 3.2 x 10mm 3um 100A, 2pk	Primesep B	3 um	3.2 mm	10 mm	100 A	Guard	£441.08
B-46.010.0310	Primesep B Column 4.6 x 10mm 3um 100A	Primesep B	3 um	4.6 mm	10 mm	100 A	Column	£829.49
B-46.050.0310	Primesep B Column 4.6 x 50mm 3um 100A	Primesep B	3 um	4.6 mm	50 mm	100 A	Column	£1,133.34
B-46.100.0310	Primesep B Column 4.6 x 100mm 3um 100A	Primesep B	3 um	4.6 mm	100 mm	100 A	Column	£1,151.71
B-46.150.0310	Primesep B Column 4.6 x 150mm 3um 100A	Primesep B	3 um	4.6 mm	150 mm	100 A	Column	£1,274.23
B-46.250.0310	Primesep B Column 4.6 x 250mm 3um 100A	Primesep B	3 um	4.6 mm	250 mm	100 A	Column	£1,415.13
B-46.G.0310	Primesep B Guard Column 4.6 x 10mm 3um 100A, 2pk	Primesep B	3 um	4.6 mm	10 mm	100 A	Guard	£441.08

Features

Primesep B is a mixed-mode column with basic ion-pairing groups embedded on long ligand chains
Enhanced retention of acidic compounds through an anion-exchange mechanism
Retains neutral compounds by a reversed phase mechanism
Resistant to dewetting in 100% aqueous mobile phase
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep B
Mode	Mixed-Mode
Phase Type	Strong basic ion-pairing groups embedded in long ligand chains
Particle Type	Fully Porous
Particle Size	3 µm, 5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
pH Limit	1.5 - 5.0
Recommended pH Operating Range	2.0 - 4.5
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography