SiELC Primesep B2 HPLC Columns

SiELC Primesep B2 is a mixed-mode HPLC column with acidic carboxylic functional groups embedded into long ligand chains. It is a weakly basic column and by using a mobile phase adjusted to a pH below 5 the column surface becomes charged. This enables retention of acidic compounds by an ion-exchange mechanism and retention of neutral compounds by a reversed phase mechanism.

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Part Number	Short Description	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
B2-46.250.0310	Primesep B2 Column 4.6 x 250mm 3um 100A	Primesep B2	L78	3 um	4.6 mm	250 mm	100 A	Column	£1,415.09
B2-05.250.0510	Primesep B2 Column 0.5 x 250mm 5um 100A	Primesep B2	L78	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
B2-05.150.0510	Primesep B2 Column 0.5 x 150mm 5um 100A	Primesep B2	L78	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
B2-46.250.0510	Primesep B2 Column 4.6 x 250mm 5um 100A	Primesep B2	L78	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
B2-32.250.0310	Primesep B2 Column 3.2 x 250mm 3um 100A	Primesep B2	L78	3 um	3.2 mm	250 mm	100 A	Column	£1,359.99
B2-46.150.0310	Primesep B2 Column 4.6 x 150mm 3um 100A	Primesep B2	L78	3 um	4.6 mm	150 mm	100 A	Column	£1,274.23
B2-05.050.0310	Primesep B2 Column 0.5 x 50mm 3um 100A	Primesep B2	L78	3 um	0.5 mm	50 mm	100 A	Column	£1,372.24
B2-05.050.0510	Primesep B2 Column 0.5 x 50mm 5um 100A	Primesep B2	L78	5 um	0.5 mm	50 mm	100 A	Column	£1,372.24
B2-05.100.0310	Primesep B2 Column 0.5 x 100mm 3um 100A	Primesep B2	L78	3 um	0.5 mm	100 mm	100 A	Column	£1,372.24
B2-05.100.0510	Primesep B2 Column 0.5 x 100mm 5um 100A	Primesep B2	L78	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
B2-05.250.0310	Primesep B2 Column 0.5 x 250mm 3um 100A	Primesep B2	L78	3 um	0.5 mm	250 mm	100 A	Column	£1,335.47
B2-10.250.0310	Primesep B2 Column 1 x 250mm 3um 100A	Primesep B2	L78	3 um	1.0 mm	250 mm	100 A	Column	£1,323.24
B2-21.250.0310	Primesep B2 Column 2.1 x 250mm 3um 100A	Primesep B2	L78	3 um	2.1 mm	250 mm	100 A	Column	£1,323.24
B2-32.150.0310	Primesep B2 Column 3.2 x 150mm 3um 100A	Primesep B2	L78	3 um	3.2 mm	150 mm	100 A	Column	£1,286.46
B2-10.150.0310	Primesep B2 Column 1 x 150mm 3um 100A	Primesep B2	L78	3 um	1.0 mm	150 mm	100 A	Column	£1,261.96
B2-21.150.0310	Primesep B2 Column 2.1 x 150mm 3um 100A	Primesep B2	L78	3 um	2.1 mm	150 mm	100 A	Column	£1,261.96
B2-46.150.0510	Primesep B2 Column 4.6 x 150mm 5um 100A	Primesep B2	L78	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
B2-32.250.0510	Primesep B2 Column 3.2 x 250mm 5um 100A	Primesep B2	L78	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
B2-10.100.0310	Primesep B2 Column 1 x 100mm 3um 100A	Primesep B2	L78	3 um	1.0 mm	100 mm	100 A	Column	£1,225.22
B2-10.250.0510	Primesep B2 Column 1 x 250mm 5um 100A	Primesep B2	L78	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
B2-21.100.0310	Primesep B2 Column 2.1 x 100mm 3um 100A	Primesep B2	L78	3 um	2.1 mm	100 mm	100 A	Column	£1,225.22
B2-21.250.0510	Primesep B2 Column 2.1 x 250mm 5um 100A	Primesep B2	L78	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
B2-32.100.0310	Primesep B2 Column 3.2 x 100mm 3um 100A	Primesep B2	L78	3 um	3.2 mm	100 mm	100 A	Column	£1,225.22
B2-46.100.0310	Primesep B2 Column 4.6 x 100mm 3um 100A	Primesep B2	L78	3 um	4.6 mm	100 mm	100 A	Column	£1,151.71
B2-46.100.0510	Primesep B2 Column 4.6 x 100mm 5um 100A	Primesep B2	L78	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
B2-32.150.0510	Primesep B2 Column 3.2 x 150mm 5um 100A	Primesep B2	L78	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
B2-10.050.0310	Primesep B2 Column 1 x 50mm 3um 100A	Primesep B2	L78	3 um	1.0 mm	50 mm	100 A	Column	£1,200.72
B2-21.050.0310	Primesep B2 Column 2.1 x 50mm 3um 100A	Primesep B2	L78	3 um	2.1 mm	50 mm	100 A	Column	£1,200.72
B2-32.050.0310	Primesep B2 Column 3.2 x 50mm 3um 100A	Primesep B2	L78	3 um	3.2 mm	50 mm	100 A	Column	£1,200.72
B2-46.050.0310	Primesep B2 Column 4.6 x 50mm 3um 100A	Primesep B2	L78	3 um	4.6 mm	50 mm	100 A	Column	£1,133.34
B2-10.150.0510	Primesep B2 Column 1 x 150mm 5um 100A	Primesep B2	L78	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
B2-21.150.0510	Primesep B2 Column 2.1 x 150mm 5um 100A	Primesep B2	L78	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
B2-32.100.0510	Primesep B2 Column 3.2 x 100mm 5um 100A	Primesep B2	L78	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
B2-10.100.0510	Primesep B2 Column 1 x 100mm 5um 100A	Primesep B2	L78	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
B2-21.100.0510	Primesep B2 Column 2.1 x 100mm 5um 100A	Primesep B2	L78	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
B2-10.050.0510	Primesep B2 Column 1 x 50mm 5um 100A	Primesep B2	L78	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
B2-21.050.0510	Primesep B2 Column 2.1 x 50mm 5um 100A	Primesep B2	L78	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
B2-32.050.0510	Primesep B2 Column 3.2 x 50mm 5um 100A	Primesep B2	L78	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
B2-46.050.0510	Primesep B2 Column 4.6 x 50mm 5um 100A	Primesep B2	L78	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
B2-10.025.0310	Primesep B2 Column 1 x 25mm 3um 100A	Primesep B2	L78	3 um	1.0 mm	25 mm	100 A	Column	£980.18
B2-10.025.0510	Primesep B2 Column 1 x 25mm 5um 100A	Primesep B2	L78	5 um	1.0 mm	25 mm	100 A	Column	£980.18
B2-21.025.0310	Primesep B2 Column 2.1 x 25mm 3um 100A	Primesep B2	L78	3 um	2.1 mm	25 mm	100 A	Column	£980.18
B2-21.025.0510	Primesep B2 Column 2.1 x 25mm 5um 100A	Primesep B2	L78	5 um	2.1 mm	25 mm	100 A	Column	£980.18
B2-32.025.0310	Primesep B2 Column 3.2 x 25mm 3um 100A	Primesep B2	L78	3 um	3.2 mm	25 mm	100 A	Column	£980.18
B2-32.025.0510	Primesep B2 Column 3.2 x 25mm 5um 100A	Primesep B2	L78	5 um	3.2 mm	25 mm	100 A	Column	£980.18
B2-10.010.0310	Primesep B2 Column 1 x 10mm 3um 100A	Primesep B2	L78	3 um	1.0 mm	10 mm	100 A	Column	£888.29
B2-10.010.0510	Primesep B2 Column 1 x 10mm 5um 100A	Primesep B2	L78	5 um	1.0 mm	10 mm	100 A	Column	£888.29
B2-21.010.0310	Primesep B2 Column 2.1 x 10mm 3um 100A	Primesep B2	L78	3 um	2.1 mm	10 mm	100 A	Column	£888.29
B2-21.010.0510	Primesep B2 Column 2.1 x 10mm 5um 100A	Primesep B2	L78	5 um	2.1 mm	10 mm	100 A	Column	£888.29
B2-32.010.0310	Primesep B2 Column 3.2 x 10mm 3um 100A	Primesep B2	L78	3 um	3.2 mm	10 mm	100 A	Column	£888.29
B2-32.010.0510	Primesep B2 Column 3.2 x 10mm 5um 100A	Primesep B2	L78	5 um	3.2 mm	10 mm	100 A	Column	£888.29
B2-46.010.0310	Primesep B2 Column 4.6 x 10mm 3um 100A	Primesep B2	L78	3 um	4.6 mm	10 mm	100 A	Column	£829.49
B2-46.010.0510	Primesep B2 Column 4.6 x 10mm 5um 100A	Primesep B2	L78	5 um	4.6 mm	10 mm	100 A	Column	£829.49
B2-05.G.0310	Primesep B2 Guard Column 0.5 x 10mm 3um 100A, 2pk	Primesep B2	L78	3 um	0.5 mm	10 mm	100 A	Guard	£441.08
B2-10.G.0310	Primesep B2 Guard Column 1 x 10mm 3um 100A, 2pk	Primesep B2	L78	3 um	1.0 mm	10 mm	100 A	Guard	£441.08
B2-21.G.0310	Primesep B2 Guard Column 2.1 x 10mm 3um 100A, 2pk	Primesep B2	L78	3 um	2.1 mm	10 mm	100 A	Guard	£441.08
B2-32.G.0310	Primesep B2 Guard Column 3.2 x 10mm 3um 100A, 2pk	Primesep B2	L78	3 um	3.2 mm	10 mm	100 A	Guard	£441.08
B2-46.G.0310	Primesep B2 Guard Column 4.6 x 10mm 3um 100A, 2pk	Primesep B2	L78	3 um	4.6 mm	10 mm	100 A	Guard	£441.08
B2-10.G.0510	Primesep B2 Guard Column 1 x 10mm 5um 100A, 2pk	Primesep B2	L78	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
B2-21.G.0510	Primesep B2 Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep B2	L78	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
B2-32.G.0510	Primesep B2 Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep B2	L78	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
B2-46.G.0510	Primesep B2 Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep B2	L78	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
B2-100.050.0510	Primesep B2 Column 10 x 50mm 5um 100A	Primesep B2	L78	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
B2-100.100.0510	Primesep B2 Column 10 x 100mm 5um 100A	Primesep B2	L78	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
B2-100.150.0510	Primesep B2 Column 10 x 150mm 5um 100A	Primesep B2	L78	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
B2-100.250.0510	Primesep B2 Column 10 x 250mm 5um 100A	Primesep B2	L78	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
B2-220.050.0510	Primesep B2 Column 22 x 50mm 5um 100A	Primesep B2	L78	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
B2-220.100.0510	Primesep B2 Column 22 x 100mm 5um 100A	Primesep B2	L78	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
B2-220.150.0510	Primesep B2 Column 22 x 150mm 5um 100A	Primesep B2	L78	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
B2-220.250.0510	Primesep B2 Column 22 x 250mm 5um 100A	Primesep B2	L78	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67

Features

Primesep B2 stationary phase has weakly basic ion-pairing groups embedded in long ligand chains
Improves retention of acidic compounds by anion-exchange mechanism
Separates bases by ion-exclusion mechanism
Retains neutral compounds by reversed phase mechanism
Offers mobile phase compatibility with LC-MS
Recommended to use ammonium acetate or ammonium formate buffers in mobile phase
Resistant to dewetting in 100% aqueous mobile phase
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep B2
Mode	Mixed-Mode
Phase Type	Weakly basic ion-pairing groups embedded in long ligand chains
Particle Type	Fully Porous
Particle Size	3 µm, 5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
pH Limit	1.5 - 7.0
Recommended pH Operating Range	2.0 - 6.5
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography