SiELC Primesep B4 HPLC Columns

SiELC Primesep B4 is a short carbon chain reversed phase mixed-mode HPLC column with embedded basic ion-pairing groups. It provides enhanced retention of acidic compounds through an anion-exchange mechanism, retains basic and acidic surfactants using a multi-mode mechanism, separates hydrophobic bases via an ion-exclusion mechanism, and retains hydrophobic neutral compounds through a reverse-phase mechanism. The short carbon chain in the Primesep B4 stationary phase allows hydrophobic compounds to elute in a shorter time compared to phases with longer chain.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
B4-05.250.0510	Primesep B4 Column 0.5 x 250mm 5um 100A	Primesep B4	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
B4-05.150.0510	Primesep B4 Column 0.5 x 150mm 5um 100A	Primesep B4	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
B4-46.250.0510	Primesep B4 Column 4.6 x 250mm 5um 100A	Primesep B4	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
B4-05.100.0510	Primesep B4 Column 0.5 x 100mm 5um 100A	Primesep B4	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
B4-46.150.0510	Primesep B4 Column 4.6 x 150mm 5um 100A	Primesep B4	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
B4-32.250.0510	Primesep B4 Column 3.2 x 250mm 5um 100A	Primesep B4	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
B4-10.250.0510	Primesep B4 Column 1 x 250mm 5um 100A	Primesep B4	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
B4-21.250.0510	Primesep B4 Column 2.1 x 250mm 5um 100A	Primesep B4	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
B4-46.100.0510	Primesep B4 Column 4.6 x 100mm 5um 100A	Primesep B4	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
B4-32.150.0510	Primesep B4 Column 3.2 x 150mm 5um 100A	Primesep B4	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
B4-10.150.0510	Primesep B4 Column 1 x 150mm 5um 100A	Primesep B4	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
B4-21.150.0510	Primesep B4 Column 2.1 x 150mm 5um 100A	Primesep B4	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
B4-32.100.0510	Primesep B4 Column 3.2 x 100mm 5um 100A	Primesep B4	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
B4-10.100.0510	Primesep B4 Column 1 x 100mm 5um 100A	Primesep B4	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
B4-21.100.0510	Primesep B4 Column 2.1 x 100mm 5um 100A	Primesep B4	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
B4-10.050.0510	Primesep B4 Column 1 x 50mm 5um 100A	Primesep B4	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
B4-21.050.0510	Primesep B4 Column 2.1 x 50mm 5um 100A	Primesep B4	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
B4-32.050.0510	Primesep B4 Column 3.2 x 50mm 5um 100A	Primesep B4	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
B4-46.050.0510	Primesep B4 Column 4.6 x 50mm 5um 100A	Primesep B4	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
B4-10.025.0510	Primesep B4 Column 1 x 25mm 5um 100A	Primesep B4	5 um	1.0 mm	25 mm	100 A	Column	£980.18
B4-21.025.0510	Primesep B4 Column 2.1 x 25mm 5um 100A	Primesep B4	5 um	2.1 mm	25 mm	100 A	Column	£980.18
B4-32.025.0510	Primesep B4 Column 3.2 x 25mm 5um 100A	Primesep B4	5 um	3.2 mm	25 mm	100 A	Column	£980.18
B4-10.010.0510	Primesep B4 Column 1 x 10mm 5um 100A	Primesep B4	5 um	1.0 mm	10 mm	100 A	Column	£888.29
B4-21.010.0510	Primesep B4 Column 2.1 x 10mm 5um 100A	Primesep B4	5 um	2.1 mm	10 mm	100 A	Column	£888.29
B4-32.010.0510	Primesep B4 Column 3.2 x 10mm 5um 100A	Primesep B4	5 um	3.2 mm	10 mm	100 A	Column	£888.29
B4-46.010.0510	Primesep B4 Column 4.6 x 10mm 5um 100A	Primesep B4	5 um	4.6 mm	10 mm	100 A	Column	£829.49
B4-10.G.0510	Primesep B4 Guard Column 1 x 10mm 5um 100A, 2pk	Primesep B4	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
B4-21.G.0510	Primesep B4 Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep B4	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
B4-32.G.0510	Primesep B4 Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep B4	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
B4-46.G.0510	Primesep B4 Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep B4	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
B4-100.050.0510	Primesep B4 Column 10 x 50mm 5um 100A	Primesep B4	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
B4-100.100.0510	Primesep B4 Column 10 x 100mm 5um 100A	Primesep B4	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
B4-100.150.0510	Primesep B4 Column 10 x 150mm 5um 100A	Primesep B4	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
B4-100.250.0510	Primesep B4 Column 10 x 250mm 5um 100A	Primesep B4	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
B4-220.050.0510	Primesep B4 Column 22 x 50mm 5um 100A	Primesep B4	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
B4-220.100.0510	Primesep B4 Column 22 x 100mm 5um 100A	Primesep B4	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
B4-220.150.0510	Primesep B4 Column 22 x 150mm 5um 100A	Primesep B4	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
B4-220.250.0510	Primesep B4 Column 22 x 250mm 5um 100A	Primesep B4	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67

Features

Primesep B4 stationary phase has basic ion-pairing groups embedded within a short carbon chain
Enhanced retention of acidic compounds through an anion-exchange mechanism
Retains and separates basic and acidic surfactants using a multi-mode mechanism
Separates hydrophobic bases via an ion-exclusion mechanism
Retains hydrophobic neutral compounds through a reverse-phase mechanism
Offers mobile phase compatibility with LC-MS
Resistant to dewetting in 100% aqueous mobile phase
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep B4
Mode	Mixed-Mode
Phase Type	Basic ion-pairing groups embedded in short carbon chains
Particle Type	Fully Porous
Particle Size	5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
pH Limit	1.5 - 5.0
Recommended pH Operating Range	2.0 - 4.5
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography