SiELC Primesep PB HPLC Columns

SiELC Primesep PB is a mixed-mode HPLC column that embeds both aromatic and basic ion-pairing groups on short ligand chains. It provides improved retention of acidic compounds through an anion-exchange mechanism, distinguishes aromatic compounds based on the variations in pi-pi interactions, and retains neutral compounds using a reversed phase mechanism. Depending on analyte properties, one, two, or all three interactions can be applied to the separation. Primesep PB is suitable for the separation of structural isomers of aromatic compounds and the degree of pi-pi interaction can be adjusted by varying the amount of acetonitrile in the mobile phase.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
PB-05.250.0510	Primesep PB Column 0.5 x 250mm 5um 100A	Primesep PB	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
PB-05.150.0510	Primesep PB Column 0.5 x 150mm 5um 100A	Primesep PB	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
PB-46.250.0510	Primesep PB Column 4.6 x 250mm 5um 100A	Primesep PB	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
PB-05.100.0510	Primesep PB Column 0.5 x 100mm 5um 100A	Primesep PB	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
PB-46.150.0510	Primesep PB Column 4.6 x 150mm 5um 100A	Primesep PB	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
PB-32.250.0510	Primesep PB Column 3.2 x 250mm 5um 100A	Primesep PB	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
PB-10.250.0510	Primesep PB Column 1 x 250mm 5um 100A	Primesep PB	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
PB-21.250.0510	Primesep PB Column 2.1 x 250mm 5um 100A	Primesep PB	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
PB-46.100.0510	Primesep PB Column 4.6 x 100mm 5um 100A	Primesep PB	5 um	4.6 mm	100 mm	100 A	Column	£1,145.58
PB-32.150.0510	Primesep PB Column 3.2 x 150mm 5um 100A	Primesep PB	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
PB-10.150.0510	Primesep PB Column 1 x 150mm 5um 100A	Primesep PB	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
PB-21.150.0510	Primesep PB Column 2.1 x 150mm 5um 100A	Primesep PB	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
PB-32.100.0510	Primesep PB Column 3.2 x 100mm 5um 100A	Primesep PB	5 um	3.2 mm	100 mm	100 A	Column	£1,133.34
PB-10.100.0510	Primesep PB Column 1 x 100mm 5um 100A	Primesep PB	5 um	1.0 mm	100 mm	100 A	Column	£1,133.34
PB-21.100.0510	Primesep PB Column 2.1 x 100mm 5um 100A	Primesep PB	5 um	2.1 mm	100 mm	100 A	Column	£1,133.34
PB-10.050.0510	Primesep PB Column 1 x 50mm 5um 100A	Primesep PB	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
PB-21.050.0510	Primesep PB Column 2.1 x 50mm 5um 100A	Primesep PB	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
PB-32.050.0510	Primesep PB Column 3.2 x 50mm 5um 100A	Primesep PB	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
PB-46.050.0510	Primesep PB Column 4.6 x 50mm 5um 100A	Primesep PB	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
PB-10.025.0510	Primesep PB Column 1 x 25mm 5um 100A	Primesep PB	5 um	1.0 mm	25 mm	100 A	Column	£980.18
PB-21.025.0510	Primesep PB Column 2.1 x 25mm 5um 100A	Primesep PB	5 um	2.1 mm	25 mm	100 A	Column	£980.18
PB-32.025.0510	Primesep PB Column 3.2 x 25mm 5um 100A	Primesep PB	5 um	3.2 mm	25 mm	100 A	Column	£980.18
PB-10.010.0510	Primesep PB Column 1 x 10mm 5um 100A	Primesep PB	5 um	1.0 mm	10 mm	100 A	Column	£888.29
PB-21.010.0510	Primesep PB Column 2.1 x 10mm 5um 100A	Primesep PB	5 um	2.1 mm	10 mm	100 A	Column	£888.29
PB-32.010.0510	Primesep PB Column 3.2 x 10mm 5um 100A	Primesep PB	5 um	3.2 mm	10 mm	100 A	Column	£888.29
PB-46.010.0510	Primesep PB Column 4.6 x 10mm 5um 100A	Primesep PB	5 um	4.6 mm	10 mm	100 A	Column	£829.49
PB-10.G.0510	Primesep PB Guard Column 1 x 10mm 5um 100A, 2pk	Primesep PB	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
PB-21.G.0510	Primesep PB Guard Column 2.1 x 10mm 5um 100A, 2pk	Primesep PB	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
PB-32.G.0510	Primesep PB Guard Column 3.2 x 10mm 5um 100A, 2pk	Primesep PB	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
PB-46.G.0510	Primesep PB Guard Column 4.6 x 10mm 5um 100A, 2pk	Primesep PB	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
PB-100.050.0510	Primesep PB Column 10 x 50mm 5um 100A	Primesep PB	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
PB-100.100.0510	Primesep PB Column 10 x 100mm 5um 100A	Primesep PB	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
PB-100.150.0510	Primesep PB Column 10 x 150mm 5um 100A	Primesep PB	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
PB-100.250.0510	Primesep PB Column 10 x 250mm 5um 100A	Primesep PB	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
PB-220.050.0510	Primesep PB Column 22 x 50mm 5um 100A	Primesep PB	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
PB-220.100.0510	Primesep PB Column 22 x 100mm 5um 100A	Primesep PB	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
PB-220.150.0510	Primesep PB Column 22 x 150mm 5um 100A	Primesep PB	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
PB-220.250.0510	Primesep PB Column 22 x 250mm 5um 100A	Primesep PB	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67

Features

Primesep PB is a mixed-mode HPLC column that embeds both aromatic and basic ion-pairing groups on a short carbon chain
Three interactions on one column - reversed phase, pi-pi, and anion exchange
Enhanced retention of acidic compounds through an anion-exchange mechanism
Differentiates aromatic compounds based on the variations in pi-pi interaction
Retains neutral compounds using a reversed phase mechanism
Suitable for the separation of structural isomers of aromatic compounds
Offers mobile phase compatibility with LC-MS
Scalable from capillary to prep
Guard columns connect directly to columns and do not require any additional holders

Specifications

	Primesep PB
Mode	Mixed-Mode
Phase Type	Embedded aromatic and basic ion-pairing groups on a short carbon chain
Particle Type	Fully Porous
Particle Size	5 µm
Pore Size	100 Å
Temperature Range	0 - 50°C
pH Limit	1.5 - 5.0
Recommended pH Operating Range	2.0 - 4.5
Pressure Limit	345 bar

Literature

SiELC Primesep Brochure (1.10 MB)

Creating new dimensions in the world of chromatography

SiELC Primesep - Pharmaceutical Analysis Brochure (477.59 kB)

Solving problems of pharmaceutical HPLC analysis using Primesep columns

SiELC Primesep - Separation of Ions Brochure (293.32 kB)

New alternative to ion chromatography