Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC Columns
Thermo Scientific Acclaim Mixed-Mode HILIC-1 column combines the advantages of reversed phase and HILIC in one column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.
- Separates both polar and non-polar molecules with selectivity complementary to reversed phase columns
- Operates in both reversed phase and HILIC modes
- Retains highly polar molecules
- Covers broader application range than conventional diol-based columns, including nonionic ethoxylated surfactants, drug metabolites, lipids, and polyethylene glycols (PEGs)
- Stable between pH 2.5 and 7.5
- Available in 3 and 5 μm particle sizes with an average pore diameter of 120 Å
- Range also includes Acclaim Mixed-Mode WCX-1 (reversed phase and weak cation exchange) and Acclaim Mixed-Mode WAX-1 (reversed phase and weak anion exchange)
Acclaim Mixed-Mode HILIC-1 | |
Mode | Mixed-Mode |
Phase Type | Hydrophobic alkyl chain with a diol group at the terminus |
Particle Type | Fully Porous |
Particle Size | 3 µm, 5 µm |
Pore Size | 120 Å |
Surface Area | 300 m2/g |
Temperature Limit | 50°C |
pH Limit | 2.5 - 7.5 |
Pressure Limit | 400 bar |