Silicycle SiliaSep Premium Flash Cartridges

Silicycle SiliaSep PREMIUM flash cartridges packed with 25 μm spherical silica gel will give you better resolution for difficult purifications, sharper peaks, higher loading capacity and faster separations. They show perfect linearity between all formats, from 4 g cartridges to XL 1600 g cartridges, to allow you to easily scale-up your methods and transfer your preliminary tests onto bigger cartridges sizes.

A wide variety of phases are available to suit your application:

C18 - Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.

Silica - Most popular sorbent for day-to-day use for the purification of non-ionic polar organic compounds.

Diol - Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.

C8 - Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.

Amine (WAX) - The amine phase can be used either in normal phase for the purification of compounds with basic properties & for monosaccharides separation; or as a weak anion exchanger (pKa of 9.8). At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.

C4 - Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.

Phenyl - Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.

PFP - For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).

Cyano - Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.

SAX nec (TMA Chloride) - The quaternary amine is permanently charged (pH independent). It is commonly used for the extraction of weak anions (such as carboxylic acids) that may not bind strongly enough to weaker anion exchangers. Analysis of acidic drugs and analgesics, biomolecules (peptides and proteins) and water-soluble vitamins (vitamins B and C).

SAX-2 nec (TMA Acetate) - The acetate counter-ion is easily exchangeable (more than the chloride ion) for compounds with pKa < 5, such as carboxylic acids. This phase can be used in organic chemistry applications to selectively purify acidic compounds or remove acidic impurities from reaction mixtures.

SCX and SCX-2 - Due to the very low pKa (< 1) these functions are strong cation exchangers since they maintain a negative charge throughout the pH scale. The most common use is likely for catch and release purification of weak cations (for example basic drugs and analgesics), analysis of basic biomolecules (peptides and proteins) and water-soluble vitamins (basic vitamins B and C).