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SiELC BIST B HPLC Columns

BIST B from SiELC is a positively charged silica-based anion-exchange HPLC column with hydrophilic properties. It is the preferred column when molecules with a very high number of positive charges need to be analysed. Typical applications include compounds such as polyimine, polylysine, and glucosamines.

BIST (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple charges. BIST is a simple way to achieve retention for compounds that are traditionally difficult to separate. A multi-charged, negative buffer acts as a bridge, linking positively charged analyte ions to the positively charged column surface. A mobile phase consisting of mostly organic solvent (such as acetonitrile) minimises the formation of a solvation layer around the charged analytes.

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Part Number Short Description Phase Particle Size ID Length Pore Size Type Price Qty
TB-05.100.0310 BIST B Column 0.50 x 100mm 3um 100A BIST B 3 um 0.5 mm 100 mm 100 A Column
£1,370.81
TB-05.100.0510 BIST B Column 0.50 x 100mm 5um 100A BIST B 5 um 0.5 mm 100 mm 100 A Column
£1,372.24
TB-05.100.0530 BIST B Column 0.50 x 100mm 5um 300A BIST B 5 um 0.5 mm 100 mm 300 A Column
£1,332.00
TB-05.150.0510 BIST B Column 0.50 x 150mm 5um 100A BIST B 5 um 0.5 mm 150 mm 100 A Column
£1,414.98
TB-05.150.0530 BIST B Column 0.50 x 150mm 5um 300A BIST B 5 um 0.5 mm 150 mm 300 A Column
£1,414.98
TB-05.250.0310 BIST B Column 0.50 x 250mm 3um 100A BIST B 3 um 0.5 mm 250 mm 100 A Column
£1,255.36
TB-05.250.0510 BIST B Column 0.50 x 250mm 5um 100A BIST B 5 um 0.5 mm 250 mm 100 A Column
£1,324.47
TB-05.250.0530 BIST B Column 0.50 x 250mm 5um 300A BIST B 5 um 0.5 mm 250 mm 300 A Column
£1,324.47
TB-10.010.0310 BIST B Column 1 x 10mm 3um 100A BIST B 3 um 1.0 mm 10 mm 100 A Column
£1,005.60
TB-10.010.0510 BIST B Column 1 x 10mm 5um 100A BIST B 5 um 1.0 mm 10 mm 100 A Column
£1,005.60
TB-10.010.0530 BIST B Column 1 x 10mm 5um 300A BIST B 5 um 1.0 mm 10 mm 300 A Column
£1,005.60
TB-10.025.0310 BIST B Column 1 x 25mm 3um 100A BIST B 3 um 1.0 mm 25 mm 100 A Column
£1,289.08
TB-10.025.0510 BIST B Column 1 x 25mm 5um 100A BIST B 5 um 1.0 mm 25 mm 100 A Column
£1,131.73
TB-10.025.0530 BIST B Column 1 x 25mm 5um 300A BIST B 5 um 1.0 mm 25 mm 300 A Column
£1,160.05
TB-10.050.0310 BIST B Column 1 x 50mm 3um 100A BIST B 3 um 1.0 mm 50 mm 100 A Column
£1,231.41
TB-10.050.0510 BIST B Column 1 x 50mm 5um 100A BIST B 5 um 1.0 mm 50 mm 100 A Column
£1,139.65
TB-10.050.0530 BIST B Column 1 x 50mm 5um 300A BIST B 5 um 1.0 mm 50 mm 300 A Column
£1,136.07
TB-10.100.0310 BIST B Column 1 x 100mm 3um 100A BIST B 3 um 1.0 mm 100 mm 100 A Column
£1,268.11
TB-10.100.0510 BIST B Column 1 x 100mm 5um 100A BIST B 5 um 1.0 mm 100 mm 100 A Column
£1,154.44
TB-10.100.0530 BIST B Column 1 x 100mm 5um 300A BIST B 5 um 1.0 mm 100 mm 300 A Column
£1,154.44
TB-10.150.0310 BIST B Column 1 x 150mm 3um 100A BIST B 3 um 1.0 mm 150 mm 100 A Column
£1,335.05
TB-10.150.0510 BIST B Column 1 x 150mm 5um 100A BIST B 5 um 1.0 mm 150 mm 100 A Column
£1,209.76
TB-10.150.0530 BIST B Column 1 x 150mm 5um 300A BIST B 5 um 1.0 mm 150 mm 300 A Column
£1,209.76
TB-10.250.0310 BIST B Column 1 x 250mm 3um 100A BIST B 3 um 1.0 mm 250 mm 100 A Column
£1,243.85
TB-10.250.0510 BIST B Column 1 x 250mm 5um 100A BIST B 5 um 1.0 mm 250 mm 100 A Column
£1,128.68
TB-10.250.0530 BIST B Column 1 x 250mm 5um 300A BIST B 5 um 1.0 mm 250 mm 300 A Column
£1,128.68
TB-10.G.0310 BIST B Guard Column 1 x 10mm 3um 100A, 2pk BIST B 3 um 1.0 mm 10 mm 100 A Guard
£853.58
TB-10.G.0510 BIST B Guard Column 1 x 10mm 5um 100A, 2pk BIST B 5 um 1.0 mm 10 mm 100 A Guard
£806.18
TB-10.G.0530 BIST B Guard Column 1 x 10mm 5um 300A, 2pk BIST B 5 um 1.0 mm 10 mm 300 A Guard
£806.18
TB-100.050.0510 BIST B Column 10 x 50mm 5um 100A BIST B 5 um 10.0 mm 50 mm 100 A Column
£2,554.05
TB-100.050.1010 BIST B Column 10 x 50mm 10um 100A BIST B 10 um 10.0 mm 50 mm 100 A Column
£2,453.24
TB-100.100.0510 BIST B Column 10 x 100mm 5um 100A BIST B 5 um 10.0 mm 100 mm 100 A Column
£3,209.37
TB-100.100.1010 BIST B Column 10 x 100mm 10um 100A BIST B 10 um 10.0 mm 100 mm 100 A Column
£2,923.73
TB-100.150.0510 BIST B Column 10 x 150mm 5um 100A BIST B 5 um 10.0 mm 150 mm 100 A Column
£3,495.04
TB-100.150.1010 BIST B Column 10 x 150mm 10um 100A BIST B 10 um 10.0 mm 150 mm 100 A Column
£3,209.37
TB-100.250.0510 BIST B Column 10 x 250mm 5um 100A BIST B 5 um 10.0 mm 250 mm 100 A Column
£4,637.63
TB-100.250.1010 BIST B Column 10 x 250mm 10um 100A BIST B 10 um 10.0 mm 250 mm 100 A Column
£4,335.19
TB-21.010.0310 BIST B Column 2.1 x 10mm 3um 100A BIST B 3 um 2.1 mm 10 mm 100 A Column
£888.29
TB-21.010.0510 BIST B Column 2.1 x 10mm 5um 100A BIST B 5 um 2.1 mm 10 mm 100 A Column
£888.29
TB-21.010.0530 BIST B Column 2.1 x 10mm 5um 300A BIST B 5 um 2.1 mm 10 mm 300 A Column
£888.29
TB-21.025-0310 BIST B Column 2.1 x 25mm 3um 100A BIST B 3 um 2.1 mm 25 mm 100 A Column
£980.18
TB-21.025.0510 BIST B Column 2.1 x 25mm 5um 100A BIST B 5 um 2.1 mm 25 mm 100 A Column
£980.18
TB-21.025.0530 BIST B Column 2.1 x 25mm 5um 300A BIST B 5 um 2.1 mm 25 mm 300 A Column
£980.18
TB-21.050.0310 BIST B Column 2.1 x 50mm 3um 100A BIST B 3 um 2.1 mm 50 mm 100 A Column
£1,200.72
TB-21.050.0510 BIST B Column 2.1 x 50mm 5um 100A BIST B 5 um 2.1 mm 50 mm 100 A Column
£1,096.57
TB-21.050.0530 BIST B Column 2.1 x 50mm 5um 300A BIST B 5 um 2.1 mm 50 mm 300 A Column
£1,096.57
TB-21.100.0310 BIST B Column 2.1 x 100mm 3um 100A BIST B 3 um 2.1 mm 100 mm 100 A Column
£1,225.22
TB-21.100.0510 BIST B Column 2.1 x 100mm 5um 100A BIST B 5 um 2.1 mm 100 mm 100 A Column
£1,133.34
TB-21.100.0530 BIST B Column 2.1 x 100mm 5um 300A BIST B 5 um 2.1 mm 100 mm 300 A Column
£1,133.34
TB-21.150.0310 BIST B Column 2.1 x 150mm 3um 100A BIST B 3 um 2.1 mm 150 mm 100 A Column
£1,261.96
TB-21.150.0510 BIST B Column 2.1 x 150mm 5um 100A BIST B 5 um 2.1 mm 150 mm 100 A Column
£1,151.71
TB-21.150.0530 BIST B Column 2.1 x 150mm 5um 300A BIST B 5 um 2.1 mm 150 mm 300 A Column
£1,151.71
TB-21.250.0310 BIST B Column 2.1 x 250mm 3um 100A BIST B 3 um 2.1 mm 250 mm 100 A Column
£1,323.24
TB-21.250.0510 BIST B Column 2.1 x 250mm 5um 100A BIST B 5 um 2.1 mm 250 mm 100 A Column
£1,200.72
TB-21.250.0530 BIST B Column 2.1 x 250mm 5um 300A BIST B 5 um 2.1 mm 250 mm 300 A Column
£1,200.72
TB-21.G.0310 BIST B Guard Column 2.1 x 10mm 3um 100A, 2pk BIST B 3 um 2.1 mm 10 mm 100 A Guard
£441.08
TB-21.G.0510 BIST B Guard Column 2.1 x 10mm 5um 100A, 2pk BIST B 5 um 2.1 mm 10 mm 100 A Guard
£416.57
TB-21.G.0530 BIST B Guard Column 2.1 x 10mm 5um 300A, 2pk BIST B 5 um 2.1 mm 10 mm 300 A Guard
£416.57
TB-220.050.0510 BIST B Column 22 x 50mm 5um 100A BIST B 5 um 22.0 mm 50 mm 100 A Column
£3,579.06
TB-220.050.1010 BIST B Column 22 x 50mm 10um 100A BIST B 10 um 22.0 mm 50 mm 100 A Column
£3,209.37
TB-220.100.0510 BIST B Column 22 x 100mm 5um 100A BIST B 5 um 22.0 mm 100 mm 100 A Column
£5,192.15
TB-220.100.1010 BIST B Column 22 x 100mm 10um 100A BIST B 10 um 22.0 mm 100 mm 100 A Column
£4,906.47
TB-220.150.0510 BIST B Column 22 x 150mm 5um 100A BIST B 5 um 22.0 mm 150 mm 100 A Column
£6,889.25
TB-220.150.1010 BIST B Column 22 x 150mm 10um 100A BIST B 10 um 22.0 mm 150 mm 100 A Column
£6,519.59
TB-220.250.0510 BIST B Column 22 x 250mm 5um 100A BIST B 5 um 22.0 mm 250 mm 100 A Column
£7,359.74
TB-220.250.1010 BIST B Column 22 x 250mm 10um 100A BIST B 10 um 22.0 mm 250 mm 100 A Column
£7,746.20
TB-32.010.0310 BIST B Column 3.2 x 10mm 3um 100A BIST B 3 um 3.2 mm 10 mm 100 A Column
£888.29
TB-32.010.0510 BIST B Column 3.2 x 10mm 5um 100A BIST B 5 um 3.2 mm 10 mm 100 A Column
£888.29
TB-32.010.0530 BIST B Column 3.2 x 10mm 5um 300A BIST B 5 um 3.2 mm 10 mm 300 A Column
£888.29
TB-32.025.0310 BIST B Column 3.2 x 25mm 3um 100A BIST B 3 um 3.2 mm 25 mm 100 A Column
£980.18
TB-32.025.0510 BIST B Column 3.2 x 25mm 5um 100A BIST B 5 um 3.2 mm 25 mm 100 A Column
£980.18
TB-32.025.0530 BIST B Column 3.2 x 25mm 5um 300A BIST B 5 um 3.2 mm 25 mm 300 A Column
£1,096.57
TB-32.025.1010 BIST B Column 3.2 x 25mm 10um 100A BIST B 10 um 3.2 mm 25 mm 100 A Column
£1,047.56
TB-32.025.1030 BIST B Column 3.2 x 25mm 10um 300A BIST B 10 um 3.2 mm 25 mm 300 A Column
£1,096.57
TB-32.050.0310 BIST B Column 3.2 x 50mm 3um 100A BIST B 3 um 3.2 mm 50 mm 100 A Column
£1,200.72
TB-32.050.0510 BIST B Column 3.2 x 50mm 5um 100A BIST B 5 um 3.2 mm 50 mm 100 A Column
£1,096.57
TB-32.050.0530 BIST B Column 3.2 x 50mm 5um 300A BIST B 5 um 3.2 mm 50 mm 300 A Column
£1,096.57
TB-32.050.1010 BIST B Column 3.2 x 50mm 10um 100A BIST B 10 um 3.2 mm 50 mm 100 A Column
£1,047.56
TB-32.050.1030 BIST B Column 3.2 x 50mm 10um 300A BIST B 10 um 3.2 mm 50 mm 300 A Column
£1,096.57
TB-32.100.0310 BIST B Column 3.2 x 100mm 3um 100A BIST B 3 um 3.2 mm 100 mm 100 A Column
£1,225.22
TB-32.100.0510 BIST B Column 3.2 x 100mm 5um 100A BIST B 5 um 3.2 mm 100 mm 100 A Column
£1,133.34
TB-32.100.0530 BIST B Column 3.2 x 100mm 5um 300A BIST B 5 um 3.2 mm 100 mm 300 A Column
£1,133.34
TB-32.100.1010 BIST B Column 3.2 x 100mm 10um 100A BIST B 10 um 3.2 mm 100 mm 100 A Column
£1,102.70
TB-32.100.1030 BIST B Column 3.2 x 100mm 10um 300A BIST B 10 um 3.2 mm 100 mm 300 A Column
£1,102.70
TB-32.150.0310 BIST B Column 3.2 x 150mm 3um 100A BIST B 3 um 3.2 mm 150 mm 100 A Column
£1,286.46
TB-32.150.0510 BIST B Column 3.2 x 150mm 5um 100A BIST B 5 um 3.2 mm 150 mm 100 A Column
£1,176.20
TB-32.150.0530 BIST B Column 3.2 x 150mm 5um 300A BIST B 5 um 3.2 mm 150 mm 300 A Column
£1,176.20
TB-32.150.1010 BIST B Column 3.2 x 150mm 10um 100A BIST B 10 um 3.2 mm 150 mm 100 A Column
£1,114.95
TB-32.150.1030 BIST B Column 3.2 x 150mm 10um 300A BIST B 10 um 3.2 mm 150 mm 300 A Column
£1,114.95
TB-32.250.0310 BIST B Column 3.2 x 250mm 3um 100A BIST B 3 um 3.2 mm 250 mm 100 A Column
£1,359.99
TB-32.250.0510 BIST B Column 3.2 x 250mm 5um 100A BIST B 5 um 3.2 mm 250 mm 100 A Column
£1,237.47
TB-32.250.0530 BIST B Column 3.2 x 250mm 5um 300A BIST B 5 um 3.2 mm 250 mm 300 A Column
£1,237.47
TB-32.250.1010 BIST B Column 3.2 x 250mm 10um 100A BIST B 10 um 3.2 mm 250 mm 100 A Column
£1,188.46
TB-32.250.1030 BIST B Column 3.2 x 250mm 10um 300A BIST B 10 um 3.2 mm 250 mm 300 A Column
£1,188.46
TB-32.G.0310 BIST B Guard Column 3.2 x 10mm 3um 100A, 2pk BIST B 3 um 3.2 mm 10 mm 100 A Guard
£441.08
TB-32.G.0510 BIST B Guard Column 3.2 x 10mm 5um 100A, 2pk BIST B 5 um 3.2 mm 10 mm 100 A Guard
£416.57
TB-32.G.0530 BIST B Guard Column 3.2 x 10mm 5um 300A, 2pk BIST B 5 um 3.2 mm 10 mm 300 A Guard
£416.57
TB-46.010.0310 BIST B Column 4.6 x 10mm 3um 100A BIST B 3 um 4.6 mm 10 mm 100 A Column
£829.49
TB-46.010.0510 BIST B Column 4.6 x 10mm 5um 100A BIST B 5 um 4.6 mm 10 mm 100 A Column
£829.49
TB-46.010.0530 BIST B Column 4.6 x 10mm 5um 300A BIST B 5 um 4.6 mm 10 mm 300 A Column
£829.49
TB-46.050.0310 BIST B Column 4.6 x 50mm 3um 100A BIST B 3 um 4.6 mm 50 mm 100 A Column
£1,133.34
TB-46.050.0510 BIST B Column 4.6 x 50mm 5um 100A BIST B 5 um 4.6 mm 50 mm 100 A Column
£1,029.19
TB-46.050.0530 BIST B Column 4.6 x 50mm 5um 300A BIST B 5 um 4.6 mm 50 mm 300 A Column
£1,029.19
TB-46.050.1010 BIST B Column 4.6 x 50mm 10um 100A BIST B 10 um 4.6 mm 50 mm 100 A Column
£1,029.19
TB-46.050.1030 BIST B Column 4.6 x 50mm 10um 300A BIST B 10 um 4.6 mm 50 mm 300 A Column
£1,029.19
TB-46.100.0310 BIST B Column 4.6 x 100mm 3um 100A BIST B 3 um 4.6 mm 100 mm 100 A Column
£1,151.71
TB-46.100.0510 BIST B Column 4.6 x 100mm 5um 100A BIST B 5 um 4.6 mm 100 mm 100 A Column
£1,133.34
TB-46.100.0530 BIST B Column 4.6 x 100mm 5um 300A BIST B 5 um 4.6 mm 100 mm 300 A Column
£1,133.34
TB-46.100.1010 BIST B Column 4.6 x 100mm 10um 100A BIST B 10 um 4.6 mm 100 mm 100 A Column
£1,133.34
TB-46.100.1030 BIST B Column 4.6 x 100mm 10um 300A BIST B 10 um 4.6 mm 100 mm 300 A Column
£1,133.34
TB-46.150.0310 BIST B Column 4.6 x 150mm 3um 100A BIST B 3 um 4.6 mm 150 mm 100 A Column
£1,274.23
TB-46.150.0510 BIST B Column 4.6 x 150mm 5um 100A BIST B 5 um 4.6 mm 150 mm 100 A Column
£1,170.08
TB-46.150.0530 BIST B Column 4.6 x 150mm 5um 300A BIST B 5 um 4.6 mm 150 mm 300 A Column
£1,170.08
TB-46.150.1010 BIST B Column 4.6 x 150mm 10um 100A BIST B 10 um 4.6 mm 150 mm 100 A Column
£1,170.08
TB-46.150.1030 BIST B Column 4.6 x 150mm 10um 300A BIST B 10 um 4.6 mm 150 mm 300 A Column
£1,170.08
TB-46.250.0310 BIST B Column 4.6 x 250mm 3um 100A BIST B 3 um 4.6 mm 250 mm 100 A Column
£1,415.21
TB-46.250.0510 BIST B Column 4.6 x 250mm 5um 100A BIST B 5 um 4.6 mm 250 mm 100 A Column
£1,280.36
TB-46.250.0530 BIST B Column 4.6 x 250mm 5um 300A BIST B 5 um 4.6 mm 250 mm 300 A Column
£1,359.99
TB-46.250.1010 BIST B Column 4.6 x 250mm 10um 100A BIST B 10 um 4.6 mm 250 mm 100 A Column
£1,359.99
TB-46.250.1030 BIST B Column 4.6 x 250mm 10um 300A BIST B 10 um 4.6 mm 250 mm 300 A Column
£1,359.99
TB-46.G.0310 BIST B Guard Column 4.6 x 10mm 3um 100A, 2pk BIST B 3 um 4.6 mm 10 mm 100 A Guard
£414.62
TB-46.G.0510 BIST B Guard Column 4.6 x 10mm 5um 100A, 2pk BIST B 5 um 4.6 mm 10 mm 100 A Guard
£416.57
TB-46.G.0530 BIST B Guard Column 4.6 x 10mm 5um 300A, 2pk BIST B 5 um 4.6 mm 10 mm 300 A Guard
£416.57
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Features
  • BIST provides an additional separation tool to a whole class of organic and inorganic charged molecules
  • BIST B has a positive surface charge and is useful for the analysis of cations
  • BIST requires a doubly charged buffer ion present in the mobile phase that are opposite in charge to that of the stationary phase surface
  • Requires reduced water in the mobile phase to minimise ion solvation
  • BIST range includes BIST B+ for more retention of positively charged molecules, and BIST A and BIST A+ for analyis of negatively charged molecules
  • Compatible with LC-MS
  • Scalable from capillary to prep
  • Guard columns connect directly to columns and do not require any additional holders
Specifications
  BIST B
Mode BIST (Bridge Ion Separation Technique)
Surface Charge Positive
Particle Type Fully Porous
Particle Size 3 µm, 5 µm, 10 µm
Pore Size 100 Å and 300 Å
Temperature Range 0 - 50°C
Pressure Limit 345 bar
Literature
SiELC BIST Brochure (1.05 MB)
BIST (Bridge Ion Separation Technology) - a new mode of LC separation
SiELC PEI Column Brochure (971.29 kB)
Measuring polyethylenimine (PEI) in biological samples
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