Chromatography Column Selection

At Crawford, we’re not tied to a particular manufacturer and will recommend the most appropriate column for your situation. Whether you’re seeking to upgrade or replace an existing chromatography method, or in need of a published application, we can recommend the perfect column.

In the following video seminar, Tony Taylor (Crawford Scientific Technical Director) takes an in-depth look at HPLC column selection strategies.

 

Download our White Paper on HPLC Column Selection using Characterisation Databases

Using our experience, we will recommend the optimal column for the method and conditions. Our technical team are highly experienced method developers with backgrounds in high-throughput commercial labs. They understand how to develop and optimise a robust, reliable method for most applications—getting the best out of the most effective products.

HPLC Column Selection

We use a range of techniques to select the optimal HPLC column. Alongside our experience and applications, we have an in-house tool which compares data for nearly all common columns obtained via the independent USP database. We use this to find columns with both similar and diverse characteristics, depending on the variety of phases needed.

GC Column Selection

Using a combination of application data, retention indexes and our experience of method development we can find the correct column for your needs. Whether you’re using capillary, capillary PLOT or packed columns—we’ll recommend you the correct column for what you’re analysing.

Solid Phase Extraction (SPE)

Our technical team has extensive method development experience in SPE which we can use to recommend the optimal solution for your molecules and matrices. We will supply you with free sample cartridges and starting conditions to allow you to get going quickly.

       Original Column      Comparison Column 1 Comparison Column 2 Comparison Column 3
Manufacturer YMC Zorbax Thermo Zorbax / Varian
Brand Triart Eclipse Plus Hypersil Gold Pursuit
Style C18 C18 C18 C18
H 0.929 1.03 0.881 1.001
H/10 0.0929 0.103 0.0881 0.1001
S -0.02 0.007 0.002 0.004
A -0.19 -0.072 -0.017 -0.166
B -0.033 -0.02 0.036 0.012
C(2.8) -0.023 -0.004 0.162 0.245
C(7.0)/10 -0.0139 0.002 0.0479 0.0226
C(7.0) -0.139 0.02 0.479 0.226
  Difference 0.20 0.52 0.40

 

H   Hydrophobicity.
S   Steric (shape).
A   Un-ionised silanols.  Can produce hydrogen bonds with bases.
B   Ability of phase to behave as base. Better retention of acids and possible repulsion of bases.
C(2.8)   Active silanols causing tailing of bases. Caused by metal ions etc. Reduced by encapping.
C(7.0)   Total ion exchange capacity. Reduced by endcapping. Effects acids and bases but not neutrals.

 

For any information or advice on any of our products - Email our Technical Team or phone 01357 522 961