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Thermo Scientific HyperSep Online SPE

Thermo Scientific HyperSep online SPE products retain specific analytes in a sample matrix when used with an appropriate HPLC column. They have been designed to clean-up and concentrate target analytes in minutes. 

HyperSep online SPE products are available in three configurations:
  -  UNIGUARD Direct-Connect Online SPE Cartridges - convenient reusable product that attaches directly to the switching valve like a HPLC column
  -  Javelin Direct-Connect Online Columns - finger-tight design for convenience and maximum efficiency
  -  HPLC Columns for Online SPE - traditional HPLC column fittings and hardware

All products are available with the following HyperSep SPE chemistries:
  -  HyperSep Retain PEP - for retention of polar and non-polar analytes
  -  HyperSep Retain CX - for retention of basic and non-polar analytes
  -  HyperSep Retain AX - for retention of acidic and non-polar analytes
  -  HyperSep Hypercarb - for retention of extremely polar analytes

Part Number Short Description SPE Format Phase Type Phase Particle Size Pore Size Material Pack Size Price Qty
60310-201 Retain PEP, 10 x 2.1mm, Javelin, 4pk Javelin Online SPE Column Reversed Phase HyperSep Retain PEP 40 - 60 um 55 - 90 A Polymeric 4
£300.18
60310-202 Retain PEP, 10 x 3mm, Javelin, 4pk Javelin Online SPE Column Reversed Phase HyperSep Retain PEP 40 - 60 um 55 - 90 A Polymeric 4
£296.68
60310-301 Retain CX, 10 x 2.1mm, Javelin, 4pk Javelin Online SPE Column SCX HyperSep Retain CX 40 - 60 um 55 - 90 A Polymeric 4
£300.18
60310-302 Retain CX, 10 x 3mm, Javelin, 4pk Javelin Online SPE Column SCX HyperSep Retain CX 40 - 60 um 55 - 90 A Polymeric 4
£300.18
60310-401 Retain AX, 10 x 2.1mm, Javelin, 4pk Javelin Online SPE Column SAX HyperSep Retain AX 40 - 60 um 55 - 90 A Polymeric 4
£300.18
60310-402 Retain AX, 10 x 3mm, Javelin, 4pk Javelin Online SPE Column SAX HyperSep Retain AX 40 - 60 um 55 - 90 A Polymeric 4
£300.18
60310-501 Hypercarb, 10 x 2.1mm, Javelin, 4pk Javelin Online SPE Column Carbon HyperSep Hypercarb 30 um Porous Graphitic Carbon 4
£322.37
60310-502 Hypercarb, 10 x 3mm, Javelin, 4pk Javelin Online SPE Column Carbon HyperSep Hypercarb 30 um Porous Graphitic Carbon 4
£309.31
60311-201 Retain PEP, 10 x 2.1mm, Drop-in Guard, 4pk Online SPE Cartridge Reversed Phase HyperSep Retain PEP 40 - 60 um 55 - 90 A Polymeric 4
£266.31
60311-202 Retain PEP, 10 x 3mm, Drop-in Guard, 4pk Online SPE Cartridge Reversed Phase HyperSep Retain PEP 40 - 60 um 55 - 90 A Polymeric 4
£270.98
60311-301 Retain CX, 10 x 2.1mm, Drop-In Guard, 4pk Online SPE Cartridge SCX HyperSep Retain CX 40 - 60 um 55 - 90 A Polymeric 4
£270.98
60311-302 Retain CX, 10 x 3mm, Drop-in Guard, 4pk Online SPE Cartridge SCX HyperSep Retain CX 40 - 60 um 55 - 90 A Polymeric 4
£270.98
60311-401 Retain AX, 10 x 2.1mm, Drop-in Guard, 4pk Online SPE Cartridge SAX HyperSep Retain AX 40 - 60 um 55 - 90 A Polymeric 4
£270.98
60311-402 Retain AX, 10 x 3mm, Drop-in Guard, 4pk Online SPE Cartridge SAX HyperSep Retain AX 40 - 60 um 55 - 90 A Polymeric 4
£270.98
60311-501 Hypercarb, 10 x 2.1mm, Drop-in Guard, 4pk Online SPE Cartridge Carbon HyperSep Hypercarb 30 um Porous Graphitic Carbon 4
£293.17
60311-502 Hypercarb, 10 x 3mm, Drop-in Guard, 4pk Online SPE Cartridge Carbon HyperSep Hypercarb 30 um Porous Graphitic Carbon 4
£293.17
60312-201 Retain PEP, 20 x 2.1mm, Online SPE column Online SPE Column Reversed Phase HyperSep Retain PEP 40 - 60 um 55 - 90 A Polymeric 1
£354.30
60312-202 Retain PEP, 20 x 3mm, Online SPE column Online SPE Column Reversed Phase HyperSep Retain PEP 40 - 60 um 55 - 90 A Polymeric 1
£374.93
60312-301 Retain CX, 20 x 2.1mm, Online SPE column Online SPE Column SCX HyperSep Retain CX 40 - 60 um 55 - 90 A Polymeric 1
£383.11
60312-302 Retain CX, 20 x 3mm, Online SPE column Online SPE Column SCX HyperSep Retain CX 40 - 60 um 55 - 90 A Polymeric 1
£374.93
60312-401 Retain AX, 20 x 2.1mm, Online SPE column Online SPE Column SAX HyperSep Retain AX 40 - 60 um 55 - 90 A Polymeric 1
£354.30
60312-402 Retain AX, 20 x 3mm, Online SPE column Online SPE Column SAX HyperSep Retain AX 40 - 60 um 55 - 90 A Polymeric 1
£374.93
60312-501 Hypercarb, 20 x 2.1mm, Online SPE Column Online SPE Column Carbon HyperSep Hypercarb 30 um Porous Graphitic Carbon 1
£381.30
60312-502 Hypercarb, 20 x 3mm, Online SPE Column Online SPE Column Carbon HyperSep Hypercarb 30 um Porous Graphitic Carbon 1
£402.97
  • Available in several convenient formats for sample preparation and provide quick and easy connection
  • Choose from HyperSep Retain PEP, Retain CX, Retain AX, and Hypercarb phases
  • Use these online SPE columns with an appropriate HPLC column and switching valve
  • Effective removal of contaminants such as proteins from samples
  • Compatible with conventional HPLC systems
  • Fast and effective clean-up and concentration of target compounds
  Retain PEP Retain AX Retain CX Hypercarb
Phase Polystyrene DVB material surface modified with urea groups Polystyrene DVB material partially modified with quaternary amine Polystyrene DVB material modified with sulfonic acid 100% porous graphitic carbon
Surface area 550 to 750 m2/g 550 to 750 m2/g 550 to 750 m2/g -
Particle size 40 to 60 µm 40 to 60 µm 40 to 60 µm 30 µm
Pore size 55 to 90 Å 55 to 90 Å 55 to 90 Å 250 Å
pH stable 0 to 14 0 to 14 0 to 14 0 to 14
Thermo Sample Preparation Catalogue 2022-2023 (7.34 MB)
Save time, improve reproducibility, and extend the lifetime of your HPLC/UHPLC and GC columns with Thermo Scientific's comprehensive range of sample preparation products
Thermo Scientific SPE Selection Poster (258.34 kB)
Learn about Thermo Scientific's polymeric, reversed phase silica, normal phase silica, and ion-exchange phase options
HyperSep Applications Notebook (8.25 MB)

Brochure containing over 100 applications using HyperSep SPE products

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