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Agilent AdvanceBio Amino Acid Analysis (AAA) Columns

Agilent’s AdvanceBio Amino Acid Analysis (AAA) delivers fast, sensitive, and reproducible separations of amino acids in protein hydrolysates and cell culture media.

AdvanceBio AAA includes proven reagents for amino acid derivatization, a ready-to-use amino acid standards kit, columns based on Agilent’s innovative Poroshell technology, and expert support from Agilent.

Alongside Agilent InfinityLab LC Series instruments, AdvanceBio AAA provides a complete solution for amino acid analysis. Agilent AdvanceBio AAA is the latest addition to the Agilent AdvanceBio family – designed as innovative solutions for biomolecule characterization.

Agilent AdvanceBio Amino Acid Analysis (AAA) columns provide fast, sensitive, and reproducible separations of amino acids in protein hydrolysates and cell culture media. Based on Agilent's innovative 2.7 µm superficially porous Poroshell technology, AdvanceBio AAA column particles are chemically modified using proprietary technology that makes them resistant to high-pH mobile phases. They are also bonded with an endcapped C18 phase that ensures excellent selectivity for amino acid analysis. 

Combining AdvanceBio AAA columns with AdvanceBio AAA reagents and online automated derivatization with Agilent analytical LC instrumentation provides an ideal, qualitative and quantitative amino acid analysis method for biopharmaceutical applications.

Part Number Short Description Phase Particle Size ID Length Type Price Qty
695975-322 AdvanceBio AAA 3 x 100mm 2.7um Column AdvanceBio AAA 2.7 um 3.0 mm 100 mm Column
£740.00
823750-946 AdvanceBio AAA 3mm 2.7um Guard 3pk AdvanceBio AAA 2.7 um 3.0 mm 5 mm Guard
£636.00
655950-802 AdvanceBio AAA 4.6 x 100mm 2.7um Column AdvanceBio AAA 2.7 um 4.6 mm 100 mm Column
£740.00
820750-931 AdvanceBio AAA 4.6mm 2.7um Guard 3pk AdvanceBio AAA 2.7 um 4.6 mm 5 mm Guard
£636.00
  • Reliable results - high resolution separations delivered by efficient Poroshell particle morphology
  • Reduced costs - long column lifetimes from robust, high-pH resistant, chemically modified silica
  • Increased flexibility - compatibility with both HPLC and UHPLC systems via 2.7 µm diameter particles
  • Quality control - AdvanceBio AAA columns are batch tested with amino acid standards to ensure quality
  • Easy ordering - standards and reagents available as kits
  • Automated online derivatization - with Agilent analytical injection systems
  AdvanceBio Amino Acid Analysis (AAA)
Bonded Phase C18
Particle Size 2.7 µm
Pore Size 100 Å
Temp Limit 60°C
pH range 3 – 11
Endcapping Double
Pressure Limit 600 Bar
AdvanceBio MS Spent Media Brochure (650.24 kB)

Agilent AdvanceBio workflows for spent media analysis

Cell Culture Analysis Workflow Ordering Guide (890.61 kB)
This workflow ordering guide will help you quickly choose and order the right columns, standards, reagents, and supplies for cell culture media analysis
AdvanceBio AAA How To Guide (1.37 MB)
Accurate results with AdvanceBio end-to-end solution
Amino Acid Application Compendium (3.86 MB)
Collection of Agilent app notes on amino acid analysis
Agilent Bio HPLC Columns (26.00 MB)

Your essential resource for biomolecule analysis

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