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Porvair Microlute CP SPE

Porvair Microlute CP takes SPE to a new level of performance by enhancing the reproducibility of analyte extraction and recovery from biological, environmental and chemical samples.

Unlike traditional loose-filled SPE methods, Microlute CP uses a hybrid structure, a solid interconnected network of evenly distributed pores combined with the retentive media. This design enhances the flow-through of samples to maximise interactions between analytes and the solid phase to deliver a reproducible SPE method that excels in performance, cleanliness and sensitivity.

-  Phases: RP, SCX, SAX, WCX, WAX
-  Formats: 96 well plates and cartridges
-  Bed Weight: 30mg/well or cartridge
-  Analysis: UHPLC, HPLC, GC, LC-MS, GC-MS
-  Applications: Drugs of abuse detection, metabolite analysis, food analysis

Part Number Short Description Bed Mass SPE Format Phase Type Phase Pack Size Price Qty
PPRP0303-050 Microlute CP SPE 30mg RP 3mL cartridge, 50pk 30 mg Cartridge Reversed Phase Microlute CP RP 50
£93.11
PPRP030P-001 Microlute CP SPE 30mg RP 96 well plate, 1pk 30 mg 96-Well Plate Reversed Phase Microlute CP RP 1
£136.56
PSAX0303-050 Microlute CP SPE 30mg SAX 3mL Cartridge, 50pk 30 mg Cartridge SAX Microlute CP SAX 50
£93.11
PSAX030P-001 Microlute CP SPE 30mg SAX 96 Well Plate, 1pk 30 mg 96-Well Plate SAX Microlute CP SAX 1
£136.56
PSCX0303-050 Microlute CP SPE 30mg SCX 3mL Cartridge, 50pk 30 mg Cartridge SCX Microlute CP SCX 50
£93.11
PSCX030P-001 Microlute CP SPE 30mg SCX 96 Well Plate, 1pk 30 mg 96-Well Plate SCX Microlute CP SCX 1
£136.56
PWAX0303-050 Microlute CP SPE 30mg WAX 3mL Cartridge, 50pk 30 mg Cartridge WAX Microlute CP WAX 50
£93.11
PWAX030P-001 Microlute CP SPE 30mg WAX 96 Well Plate, 1pk 30 mg 96-Well Plate WAX Microlute CP WAX 1
£155.18
PWCX0303-050 Microlute CP SPE 30mg WCX 3mL Cartridge, 50pk 30 mg Cartridge WCX Microlute CP WCX 50
£93.11
PWCX030P-001 Microlute CP SPE 30mg WCX 96 Well Plate, 1pk 30 mg 96-Well Plate WCX Microlute CP WCX 1
£155.18
  • Increased reproducibility of recovery levels
  • Increased sample throughput
  • No liquid channelling
  • Less resin required - allows smaller samples sizes with smaller volumes of solvents and reduced drying time
  • RP for hydrophobic retention of neutral compounds
  • Strong anion exchange for weak acidic analytes
  • Strong cation exchange for weak basic analytes
  • Weak anion exchange for strongly acidic analytes
  • Weak cation exchange for strongly basic analytes

Microlute CP RP is a polymer based phase that is ideal for retention of neutral compounds through hydrophobic interaction with some retention of polars. It is an ideal first start phase for many applications or where there is a wide range of compounds present.

Microlute CP SCX uses a sulphonic acid functional group on a polymeric base with a pKa of <1. This provides a wide pH range for cationic exchange with basic compounds and retention of neutral compounds through the base polymer.

Microlute CP SAX uses a quarternary ammonium chemistry on a polymeric base with a pKA >18. Ideal for the capture of acidic analytes through anion exchange. As with the SCX, the polymer base offers a secondary retention of neutral compounds.

Microlute CP WCX is a weak cation exchange used to retain strongly basic compounds which are always ionised at any pH. It uses a carboxylic acid ligand with a pKa ~4.5 which allows ionisation and neutralisation of the resin to allow retention and controlled release of the strong basic compounds. This is combined with the polymeric base to allow a degree of neutral compound retention.

Microlute CP WAX is a weak anion exchange used to retain strongly acidic compounds which are always ionised at any pH. It uses a tertiary amine ligand on the polymer base with a pKa ~8.5 which allows ionisation and neutralisation of the resin to allow retention and controlled release of the strong acidic compounds. This is combined with the polymeric base to allow a degree of neutral compound retention.

Porvair Microlute Sample Preparation Brochure (5.50 MB)
Innovative range of sample preparation products designed and manufactured to deliver uncompromised chromatographic performance with a whole new level of reliability and reproducibility
Porvair SPE Top 10 Tips (1.08 MB)
Top tips for using Porvair Microlute CP SPE
Microlute CP RP Protocol (640.99 kB)
Example method for the extraction of neutral compounds using Porvair Microlute CP RP
Microlute CP SAX Protocol (1.24 MB)
Example method for the extraction of weak acids using Porvair Microlute CP SAX
Microlute CP SCX Protocol (1.23 MB)
Example method for the extraction of weak bases using Porvair Microlute CP SCX
Microlute CP WAX Protocol (1.23 MB)
Example method for the extraction of strong acids using Porvair Microlute CP WAX
Microlute CP WCX Protocol (1.24 MB)
Example method for the extraction of strong bases using Porvair Microlute CP WCX
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