Porvair Microlute CP SPE
Porvair Microlute CP takes SPE to a new level of performance by enhancing the reproducibility of analyte extraction and recovery from biological, environmental and chemical samples.
Unlike traditional loose-filled SPE methods, Microlute CP uses a hybrid structure, a solid interconnected network of evenly distributed pores combined with the retentive media. This design enhances the flow-through of samples to maximise interactions between analytes and the solid phase to deliver a reproducible SPE method that excels in performance, cleanliness and sensitivity.
- Phases: RP, SCX, SAX, WCX, WAX
- Formats: 96 well plates and cartridges
- Bed Weight: 30mg/well or cartridge
- Analysis: UHPLC, HPLC, GC, LC-MS, GC-MS
- Applications: Drugs of abuse detection, metabolite analysis, food analysis
- Increased reproducibility of recovery levels
- Increased sample throughput
- No liquid channelling
- Less resin required - allows smaller samples sizes with smaller volumes of solvents and reduced drying time
- RP for hydrophobic retention of neutral compounds
- Strong anion exchange for weak acidic analytes
- Strong cation exchange for weak basic analytes
- Weak anion exchange for strongly acidic analytes
- Weak cation exchange for strongly basic analytes
Microlute CP RP is a polymer based phase that is ideal for retention of neutral compounds through hydrophobic interaction with some retention of polars. It is an ideal first start phase for many applications or where there is a wide range of compounds present.
Microlute CP SCX uses a sulphonic acid functional group on a polymeric base with a pKa of <1. This provides a wide pH range for cationic exchange with basic compounds and retention of neutral compounds through the base polymer.
Microlute CP SAX uses a quarternary ammonium chemistry on a polymeric base with a pKA >18. Ideal for the capture of acidic analytes through anion exchange. As with the SCX, the polymer base offers a secondary retention of neutral compounds.
Microlute CP WCX is a weak cation exchange used to retain strongly basic compounds which are always ionised at any pH. It uses a carboxylic acid ligand with a pKa ~4.5 which allows ionisation and neutralisation of the resin to allow retention and controlled release of the strong basic compounds. This is combined with the polymeric base to allow a degree of neutral compound retention.
Microlute CP WAX is a weak anion exchange used to retain strongly acidic compounds which are always ionised at any pH. It uses a tertiary amine ligand on the polymer base with a pKa ~8.5 which allows ionisation and neutralisation of the resin to allow retention and controlled release of the strong acidic compounds. This is combined with the polymeric base to allow a degree of neutral compound retention.