Obelisc

Obelisc HPLC Columns from SiELC

This range consists of two complimentary columns (Obelisc R and Obelisc N) which are based on Liquid Separation Cell (LiSC) technology.

Obelisc R, has reversed-phase characteristics whereas Obelisc N is used for normal phase methods.

These columns differ in the type and proximity of their charged groups and the hydrophobicity of their long chains. Obelisc R has cationic groups close to the silica surface separated from anionic groups by a hydrophobic chain in comparison to Obelisc N which has anions close to the surface separated from cationic groups by a hydrophilic chain.

Buffer concentration, pH and organic modifier concentration in the mobile phase can be adjusted to alter the properties of the stationary phase and enable separation of complex mixtures.

Obelisc All pictures shown are for illustration purpose only. Actual product may vary.

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Features

Two columns based on LiSC technology, Obelisc R and Obelisc N, separate polar and non-polar compounds using multiple separation mechanisms. 

This cost effective duo replaces multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction LIquid Chromatography) columns. Method development is simplified by using only two Obelisc columns and a few simple mass spec or low UV (<220 nm) compatible mobile phases.  Obelisc R, with reversed-phase characteristics, and Obelisc N, with normal-phase characteristics, differ in the type and proximity of their charged groups and the hydrophobicity of their long chains. Obelisc R has cationic groups close to the silica surface separated from anionic groups by a hydrophobic chain.  Obelisc N has anions close to the surface separated from cationic groups by a hydrophilic chain.

 

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