SiELC PEI HPLC Columns
SiELC PEI column is an application specific column that combines both ion-exclusion and size-exclusion mechanisms for the effective separation of polyethylenimine (PEI) polymers from other compounds, as well as from excess Cu (II) ions. PEI poses a challenge for HPLC analysis as it is not a single compound, but a mixture of different molecules of varying lengths and branching structures. It also carries multiple charges in acidic and neutral pH and can bind irreversibly to silica-based columns. It lacks a UV chromophore and so cannot by measured by a UV-Vis detector. To overcome these challenges SiELC developed the PEI column. This provides a simple and reliable method for PEI quantitation in any liquid sample. The method is based on forming a PEI complex with Cu (II), which can be detected at 285 nm in UV or 630 nm in the visible spectra.
- Combines both ion-exclusion and size-exclusion mechanisms
- Tailored LC phase for challenging PEI applications
- PEI complex exhibits strong UV and visible light absorption maxima
- Simple sample preparation includes mixing the unknown with Cu (II) stock solution followed by HPLC separation
- Sensitivity (LOQ) down to 10 ppm of PEI in samples routinely achieved
- If using ELSD, CAD or ESI-MS the copper (II) complex can be removed and unadulterated PEI samples can be injected directly
- When polymer and peptide samples may co-elute with PEI it is recommended by use the SiELC BIST B column
| PEI | |
| Mode | Mixed-Mode |
| Particle Type | Fully Porous |
| Particle Size | 5 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Range | 1.5 - 5.0 |
| Pressure Limit | 345 bar |
