Thermo Scientific MAbPac SCX-10 Columns
Thermo Scientific MAbPac SCX-10 columns are designed specifically for high-resolution, high efficiency separation of monoclonal antibodies and their variants. The non-porous pellicular resin provides high resolution, permitting the separation of monoclonal antibody variants that differ by as little as one charge. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations.
The MAbPac SCX-10 columns are available in several particle sizes. 10 µm particle size columns are available in PEEK while 5 µm columns are available in both PEEK and PEEK-lined stainless steel housings for UHPLC based rapid separation (RS) applications. The MAbPac SCX-10 5 µm shorter columns are recommended for fast separations and are ideal for high throughput needs. Longer columns provide greater resolution.
The MAbPac SCX-10RS columns are packed in PEEK-lined stainless steel columns for UHPLC operating compatibility. High flow rates can be used to achieve faster mAb analysis. These RS columns are suitable for operation up to 7000 psi. The RS bioinert column bodies prevent metal interferences which can result in comprimsed mAb separations. The RS columns are designed for use with bioinert UHPLC systems.
- Exceptionally high resolution for mAb variants
- Ideal for characterization and quality control assessment of mAbs
- Well-suited for stability studies, mAb therapeutic method development and high-throughput screening
- Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing
- Meets the regulatory requirements for biopharmaceutical characterization
- MAbPac SCX-10RS has specially developed bio-inert PEEK lined stainless steel column hardware
- MAbPac SCX-10RS recommended for high throughput analysis using UHPLC
- Provides complementary selectivity to ProPac WCX-10 for various mAb separations
| MAbPac SCX-10 | MAbPac SCX-10RS | |
| Phase | Strong Cation Exchange | Strong Cation Exchange |
| Particle Type | Polymeric | Polymeric |
| Particle Size | 5 µm and 10 µm | 5 µm |
| Pore Size | Non-porous | Non-porous |
| pH Range | 2 - 12 | 2 - 12 |
| Maximum Temperature | 60°C | 60°C |
| Hardware | PEEK | PEEK-Lined Stainless Steel |
| Pressure Limit | 10 μm: 3000 psi 5 μm: 5000 psi |
7000 psi |