Thermo Scientific MAbPac SEC-1 Columns

Thermo Scientific MAbPac SEC-1 columns are designed for high resolution separation of monoclonal antibody (mAb) analysis, including the monomers, aggregates and Fab and Fc fragments resulting from proteolysis. These size exclusion columns provide superior performance, even under nondenaturing conditions, and in high and low salt mobile phases and volatile eluents.

Based on high purity, spherical porous 300Å 5µm silica particles that are covalently modified with a hydrophilic layer, this column offers separations with very low levels of secondary interaction. The stationary phase is designed to handle different eluent conditions containing both high and low ionic strength mobile phases, as well as mass spectrometry friendly volatile eluents.

Different column formats meet different analytical needs. The 4.0mm ID column enables baseline separation of mAb monomer and dimer. The 2.1mm ID column uses low flow rate and low sample loading, making it the perfect format for MS detection.

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Buy

Part Number	Short Description	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
088789	MAbPac SEC-1, 5um, 2.1 x 300mm, Column	MAbPac SEC-1	L59	5 um	2.1 mm	300 mm	300 A	Column	£1,168.67
088790	MAbPac SEC-1, 5um, 2.1 x 150mm, Column	MAbPac SEC-1	L59	5 um	2.1 mm	150 mm	300 A	Column	£987.89
074696	MAbPac SEC-1, 5um, 4 x 300mm, Column, 1pk	MAbPac SEC-1	L59	5 um	4.0 mm	300 mm	300 A	Column	£1,302.80
074697	MAbPac SEC-1G, 5um, 4 x 50mm, Guard Column, 1pk	MAbPac SEC-1	L59	5 um	4.0 mm	50 mm	300 A	Guard	£435.05
075592	MAbPac SEC-1, 5um, 4 x 150mm, Column, 1pk	MAbPac SEC-1	L59	5 um	4.0 mm	150 mm	300 A	Column	£987.89

Features

Hydrophilic bonded layer minimises undesired interactions between the biomolecules and the stationary phase
2.1mm I.D. columns are packed into stainless steel housing
4mm I.D. columns are packed into PEEK housing
Non-metallic and biocompatible PEEK housing for 4mm ID columns eliminates metal contamination from the column hardware
Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and CAD detection
Rugged column packing provides reliability, reproducibility and long column life
Columns are available in 5µm particle size and 150 and 300mm lengths, with an average pore diameter of 300Å
Separation range for globular proteins is 1 – 1,000 kDa
USP designation of L59

Specifications

	MAbPac SEC-1
Phase	Proprietary Diol
Particle Type	Fully Porous Silica
Particle Size	5 µm
Pore Size	300 Å
Molecular Weight Lower Limit	1000 Da
Molecular Weight Upper Limit	1,000,000 Da
pH Range	2.5 − 7.5
Maximum Temperature	30°C
Hardware	PEEK for 4.0mm ID columns Stainless Steel for 2.1mm ID columns
USP Designation	L59