Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC Columns
Thermo Scientific Acclaim Mixed-Mode HILIC-1 column combines the advantages of reversed phase and HILIC in one column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.
- Separates both polar and non-polar molecules with selectivity complementary to reversed phase columns
- Operates in both reversed phase and HILIC modes
- Retains highly polar molecules
- Covers broader application range than conventional diol-based columns, including nonionic ethoxylated surfactants, drug metabolites, lipids, and polyethylene glycols (PEGs)
- Stable between pH 2.5 and 7.5
- Available in 3 and 5 μm particle sizes with an average pore diameter of 120 Å
- Range also includes Acclaim Mixed-Mode WCX-1 (reversed phase and weak cation exchange) and Acclaim Mixed-Mode WAX-1 (reversed phase and weak anion exchange)
| Acclaim Mixed-Mode HILIC-1 | |
| Mode | Mixed-Mode |
| Phase Type | Hydrophobic alkyl chain with a diol group at the terminus |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m2/g |
| Temperature Limit | 50°C |
| pH Limit | 2.5 - 7.5 |
| Pressure Limit | 400 bar |
Analytical UHPLC and HPLC Columns Brochure
(11.02 MB)
Introduction to the broad range of chemistries and column families from Thermo Scientific
Thermo Scientific HILIC Separations Guide
(14.08 MB)
Practical guide to HILIC mechanisms, method development and troubleshooting
Acclaim Mixed-Mode HILIC-1 Product Specifications
(330.66 kB)
A powerful tool for separating polar molecules