Thermo Scientific Acclaim Mixed-Mode WAX-1 HPLC Columns

Thermo Scientific Acclaim Mixed-Mode WAX-1 HPLC columns provide powerful selectivity control for the separation of anionic molecules. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.

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Part Number	Short Description	Phase Type	Phase	USP	Particle Size	ID	Length	Pore Size	Type	Price
064984	Acclaim Mixed-Mode WAX-1, 5um, 4.6 x 150mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	4.6 mm	150 mm	120 A	Column	£601.86
064985	Acclaim Mixed-Mode WAX-1, 5um, 4.6 x 250mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	4.6 mm	250 mm	120 A	Column	£698.05
067084	Acclaim Mixed-Mode WAX-1, 5um, 2.1 x 150mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	2.1 mm	150 mm	120 A	Column	£601.86
069686	Acclaim Mixed-Mode WAX-1, 5um, 2.1 x 10mm, Guard Cartridge, 2pk	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	2.1 mm	10 mm	120 A	Guard	£160.91
069704	Acclaim Mixed-Mode WAX-1, 5um, 4.6 x 10mm, Guard Cartridge, 2pk	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	4.6 mm	10 mm	120 A	Guard	£160.91
070088	Acclaim Mixed-Mode WAX-1, 3um, 3 x 150mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	3 um	3.0 mm	150 mm	120 A	Column	£638.09
070089	Acclaim Mixed-Mode WAX-1, 3um, 2.1 x 150mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	3 um	2.1 mm	150 mm	120 A	Column	£638.09
071908	Acclaim Mixed-Mode WAX-1, 3um, 3 x 50mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	3 um	3.0 mm	50 mm	120 A	Column	£515.37
071909	Acclaim Mixed-Mode WAX-1, 5um, 3 x 10mm, Guard Cartridge, 2pk	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	3.0 mm	10 mm	120 A	Guard	£160.91
088785	Acclaim Mixed-Mode WAX-1, 5um, 10 x 250mm	Mixed-Mode	Acclaim Mixed-Mode WAX-1	L78	5 um	10.0 mm	250 mm	120 A	Column	£3,884.59

Features

Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes
Stationary phase has hydrophobic alkyl chain with an anion terminus
Selectivity orthogonal to reversed-phase columns
Powerful selectivity for the separation of difficult mixtures of neutral and anionic analytes
Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids
Excellent column efficiency and peak symmetry
Stable between pH 2.5 and 7.5
Available in 3 µm and 5 μm particle sizes with an average pore diameter of 120 Å
USP classification of L78
Range also includes Acclaim Mixed-Mode WCX-1 (reversed phase and weak cation exchange) and Acclaim Mixed-Mode HILIC-1 (reversed phase and HILIC)

Specifications

	Acclaim Mixed-Mode WAX-1
Mode	Mixed-Mode
Phase Type	Hydrophobic alkyl chain with an anion terminus
Particle Type	Fully Porous
Particle Size	3 µm, 5 µm
Pore Size	120 Å
Surface Area	300 m²/g
Temperature Limit	50°C
pH Limit	2.5 - 7.5
Pressure Limit	400 bar
USP Designation	L78

Literature

Analytical UHPLC and HPLC Columns Brochure (11.02 MB)

Introduction to the broad range of chemistries and column families from Thermo Scientific

Acclaim Mixed-Mode Brochure (5.52 MB)

Columns for challenging separation needs

Acclaim-Mixed-Mode-WAX-1-Product-Specifications (474.21 kB)

Total control of selectivity

Acclaim Mixed-Mode WAX-1 Product Manual (902.24 kB)

User guide for the Acclaim Mixed-Mode WAX-1 columns

Acclaim Column Selection Guide (96.31 kB)

Quick guide to choosing your Acclaim phase by application