SHARC Mixed-Mode HPLC Columns from SiELC
SHARC HPLC columns from SiELC are the first commercially available with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Chromatography. They use the hydrogen bonding interaction of hydrogen atoms within the stationary phase with analyte molecules containing electronegative atoms such as oxygen, nitrogen, fluorine. This is typically a weak interaction, especially when the separation is performed in aqueous solution. However, used in conjunction with purely organic eluent systems, these hydrogen bonding interactions can be exploited to achieve alternative selectivity than that achieved with reversed phase, normal phase, or other mixed-mode separations.