SiELC SHARC 1 HPLC Columns

The SHARC 1 column from SiELC is the inaugural member of the SHARC series where separation is primarily based on hydrogen bonding. SHARC is an acronym for Specific Hydrogen-bond Adsorption Resolution Chromatography. A wide range of molecules with a functional group containing oxygen and nitrogen can be retained and separated from similar compounds using SHARC technology.

The operational conditions for the SHARC 1 column are unique. A blend of acetonitrile, a weak solvent, and methanol, a strong solvent, is used as the mobile phase. Pure acetonitrile forms very insignificant hydrogen bonds with the SHARC stationary phase, while methanol interacts strongly, reducing the retention of analytes based on its capacity to form hydrogen bonds. By altering the solvent ratio, an optimal retention profile can be achieved for a wide range of molecules, offering high selectivity, peak shape, efficiency, and speed.

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Part Number	Short Description	Phase	Particle Size	ID	Length	Pore Size	Type	Price
SH1-05.250.0310	SHARC 1 Column 0.5 x 250mm 3um 100A	SHARC 1	3 um	0.5 mm	250 mm	100 A	Column	£1,519.27
SH1-46.250.0310	SHARC 1 Column 4.6 x 250mm 3um 100A	SHARC 1	3 um	4.6 mm	250 mm	100 A	Column	£1,415.13
SH1-05.250.0510	SHARC 1 Column 0.5 x 250mm 5um 100A	SHARC 1	5 um	0.5 mm	250 mm	100 A	Column	£1,409.00
SH1-05.150.0510	SHARC 1 Column 0.5 x 150mm 5um 100A	SHARC 1	5 um	0.5 mm	150 mm	100 A	Column	£1,372.24
SH1-46.250.0510	SHARC 1 Column 4.6 x 250mm 5um 100A	SHARC 1	5 um	4.6 mm	250 mm	100 A	Column	£1,280.36
SH1-46.150.0310	SHARC 1 Column 4.6 x 150mm 3um 100A	SHARC 1	3 um	4.6 mm	150 mm	100 A	Column	£1,286.46
SH1-32.250.0310	SHARC 1 Column 3.2 x 250mm 3um 100A	SHARC 1	3 um	3.2 mm	250 mm	100 A	Column	£1,359.99
SH1-05.100.0510	SHARC 1 Column 0.5 x 100mm 5um 100A	SHARC 1	5 um	0.5 mm	100 mm	100 A	Column	£1,372.24
SH1-46.100.0310	SHARC 1 Column 4.6 x 100mm 3um 100A	SHARC 1	3 um	4.6 mm	100 mm	100 A	Column	£1,274.23
SH1-10.250.0310	SHARC 1 Column 1 x 250mm 3um 100A	SHARC 1	3 um	1.0 mm	250 mm	100 A	Column	£1,323.24
SH1-21.250.0310	SHARC 1 Column 2.1 x 250mm 3um 100A	SHARC 1	3 um	2.1 mm	250 mm	100 A	Column	£1,323.24
SH1-32.150.0310	SHARC 1 Column 3.2 x 150mm 3um 100A	SHARC 1	3 um	3.2 mm	150 mm	100 A	Column	£1,286.46
SH1-10.150.0310	SHARC 1 Column 1 x 150mm 3um 100A	SHARC 1	3 um	1.0 mm	150 mm	100 A	Column	£1,261.96
SH1-21.150.0310	SHARC 1 Column 2.1 x 150mm 3um 100A	SHARC 1	3 um	2.1 mm	150 mm	100 A	Column	£1,261.96
SH1-32.100.0310	SHARC 1 Column 3.2 x 100mm 3um 100A	SHARC 1	3 um	3.2 mm	100 mm	100 A	Column	£1,286.46
SH1-10.100.0310	SHARC 1 Column 1 x 100mm 3um 100A	SHARC 1	3 um	1.0 mm	100 mm	100 A	Column	£1,274.23
SH1-21.100.0310	SHARC 1 Column 2.1 x 100mm 3um 100A	SHARC 1	3 um	2.1 mm	100 mm	100 A	Column	£1,274.23
SH1-46.150.0510	SHARC 1 Column 4.6 x 150mm 5um 100A	SHARC 1	5 um	4.6 mm	150 mm	100 A	Column	£1,170.08
SH1-32.250.0510	SHARC 1 Column 3.2 x 250mm 5um 100A	SHARC 1	5 um	3.2 mm	250 mm	100 A	Column	£1,237.47
SH1-10.250.0510	SHARC 1 Column 1 x 250mm 5um 100A	SHARC 1	5 um	1.0 mm	250 mm	100 A	Column	£1,200.72
SH1-21.250.0510	SHARC 1 Column 2.1 x 250mm 5um 100A	SHARC 1	5 um	2.1 mm	250 mm	100 A	Column	£1,200.72
SH1-46.100.0510	SHARC 1 Column 4.6 x 100mm 5um 100A	SHARC 1	5 um	4.6 mm	100 mm	100 A	Column	£1,133.34
SH1-32.150.0510	SHARC 1 Column 3.2 x 150mm 5um 100A	SHARC 1	5 um	3.2 mm	150 mm	100 A	Column	£1,176.20
SH1-10.050.0310	SHARC 1 Column 1 x 50mm 3um 100A	SHARC 1	3 um	1.0 mm	50 mm	100 A	Column	£1,200.72
SH1-21.050.0310	SHARC 1 Column 2.1 x 50mm 3um 100A	SHARC 1	3 um	2.1 mm	50 mm	100 A	Column	£1,200.72
SH1-32.050.0310	SHARC 1 Column 3.2 x 50mm 3um 100A	SHARC 1	3 um	3.2 mm	50 mm	100 A	Column	£1,200.72
SH1-46.050.0310	SHARC 1 Column 4.6 x 50mm 3um 100A	SHARC 1	3 um	4.6 mm	50 mm	100 A	Column	£1,133.34
SH1-10.150.0510	SHARC 1 Column 1 x 150mm 5um 100A	SHARC 1	5 um	1.0 mm	150 mm	100 A	Column	£1,151.71
SH1-21.150.0510	SHARC 1 Column 2.1 x 150mm 5um 100A	SHARC 1	5 um	2.1 mm	150 mm	100 A	Column	£1,151.71
SH1-32.100.0510	SHARC 1 Column 3.2 x 100mm 5um 100A	SHARC 1	5 um	3.2 mm	100 mm	100 A	Column	£1,145.58
SH1-10.100.0510	SHARC 1 Column 1 x 100mm 5um 100A	SHARC 1	5 um	1.0 mm	100 mm	100 A	Column	£1,145.58
SH1-21.100.0510	SHARC 1 Column 2.1 x 100mm 5um 100A	SHARC 1	5 um	2.1 mm	100 mm	100 A	Column	£1,145.58
SH1-10.050.0510	SHARC 1 Column 1 x 50mm 5um 100A	SHARC 1	5 um	1.0 mm	50 mm	100 A	Column	£1,096.57
SH1-21.050.0510	SHARC 1 Column 2.1 x 50mm 5um 100A	SHARC 1	5 um	2.1 mm	50 mm	100 A	Column	£1,096.57
SH1-32.050.0510	SHARC 1 Column 3.2 x 50mm 5um 100A	SHARC 1	5 um	3.2 mm	50 mm	100 A	Column	£1,096.57
SH1-46.050.0510	SHARC 1 Column 4.6 x 50mm 5um 100A	SHARC 1	5 um	4.6 mm	50 mm	100 A	Column	£1,029.19
SH1-10.025.0310	SHARC 1 Column 1 x 25mm 3um 100A	SHARC 1	3 um	1.0 mm	25 mm	100 A	Column	£1,078.19
SH1-21.025.0310	SHARC 1 Column 2.1 x 25mm 3um 100A	SHARC 1	3 um	2.1 mm	25 mm	100 A	Column	£1,078.19
SH1-32.025.0310	SHARC 1 Column 3.2 x 25mm 3um 100A	SHARC 1	3 um	3.2 mm	25 mm	100 A	Column	£1,078.19
SH1-10.025.0510	SHARC 1 Column 1 x 25mm 5um 100A	SHARC 1	5 um	1.0 mm	25 mm	100 A	Column	£980.18
SH1-21.025.0510	SHARC 1 Column 2.1 x 25mm 5um 100A	SHARC 1	5 um	2.1 mm	25 mm	100 A	Column	£980.18
SH1-32.025.0510	SHARC 1 Column 3.2 x 25mm 5um 100A	SHARC 1	5 um	3.2 mm	25 mm	100 A	Column	£980.18
SH1-10.010.0310	SHARC 1 Column 1 x 10mm 3um 100A	SHARC 1	3 um	1.0 mm	10 mm	100 A	Column	£955.67
SH1-21.010.0310	SHARC 1 Column 2.1 x 10mm 3um 100A	SHARC 1	3 um	2.1 mm	10 mm	100 A	Column	£955.67
SH1-32.010.0310	SHARC 1 Column 3.2 x 10mm 3um 100A	SHARC 1	3 um	3.2 mm	10 mm	100 A	Column	£955.67
SH1-46.010.0310	SHARC 1 Column 4.6 x 10mm 3um 100A	SHARC 1	3 um	4.6 mm	10 mm	100 A	Column	£906.66
SH1-10.010.0510	SHARC 1 Column 1 x 10mm 5um 100A	SHARC 1	5 um	1.0 mm	10 mm	100 A	Column	£888.29
SH1-21.010.0510	SHARC 1 Column 2.1 x 10mm 5um 100A	SHARC 1	5 um	2.1 mm	10 mm	100 A	Column	£888.29
SH1-32.010.0510	SHARC 1 Column 3.2 x 10mm 5um 100A	SHARC 1	5 um	3.2 mm	10 mm	100 A	Column	£888.29
SH1-46.010.0510	SHARC 1 Column 4.6 x 10mm 5um 100A	SHARC 1	5 um	4.6 mm	10 mm	100 A	Column	£829.49
SH1-05.G.0310	SHARC 1 Guard Column 0.5 x 10mm 3um 100A, 2pk	SHARC 1	3 um	0.5 mm	10 mm	100 A	Guard	£441.08
SH1-10.G.0310	SHARC 1 Guard Column 1 x 10mm 3um 100A, 2pk	SHARC 1	3 um	1.0 mm	10 mm	100 A	Guard	£441.08
SH1-21.G.0310	SHARC 1 Guard Column 2.1 x 10mm 3um 100A, 2pk	SHARC 1	3 um	2.1 mm	10 mm	100 A	Guard	£441.08
SH1-32.G.0310	SHARC 1 Guard Column 3.2 x 10mm 3um 100A, 2pk	SHARC 1	3 um	3.2 mm	10 mm	100 A	Guard	£441.08
SH1-46.G.0310	SHARC 1 Guard Column 4.6 x 10mm 3um 100A, 2pk	SHARC 1	3 um	4.6 mm	10 mm	100 A	Guard	£441.08
SH1-10.G.0510	SHARC 1 Guard Column 1 x 10mm 5um 100A, 2pk	SHARC 1	5 um	1.0 mm	10 mm	100 A	Guard	£416.57
SH1-21.G.0510	SHARC 1 Guard Column 2.1 x 10mm 5um 100A, 2pk	SHARC 1	5 um	2.1 mm	10 mm	100 A	Guard	£416.57
SH1-32.G.0510	SHARC 1 Guard Column 3.2 x 10mm 5um 100A, 2pk	SHARC 1	5 um	3.2 mm	10 mm	100 A	Guard	£416.57
SH1-46.G.0510	SHARC 1 Guard Column 4.6 x 10mm 5um 100A, 2pk	SHARC 1	5 um	4.6 mm	10 mm	100 A	Guard	£416.57
SH1-100.050.0510	SHARC 1 Column 10 x 50mm 5um 100A	SHARC 1	5 um	10.0 mm	50 mm	100 A	Column	£2,839.72
SH1-100.100.0510	SHARC 1 Column 10 x 100mm 5um 100A	SHARC 1	5 um	10.0 mm	100 mm	100 A	Column	£3,394.20
SH1-100.150.0510	SHARC 1 Column 10 x 150mm 5um 100A	SHARC 1	5 um	10.0 mm	150 mm	100 A	Column	£3,965.51
SH1-100.250.0510	SHARC 1 Column 10 x 250mm 5um 100A	SHARC 1	5 um	10.0 mm	250 mm	100 A	Column	£5,192.15
SH1-220.050.0510	SHARC 1 Column 22 x 50mm 5um 100A	SHARC 1	5 um	22.0 mm	50 mm	100 A	Column	£5,091.33
SH1-220.100.0510	SHARC 1 Column 22 x 100mm 5um 100A	SHARC 1	5 um	22.0 mm	100 mm	100 A	Column	£5,847.47
SH1-220.150.0510	SHARC 1 Column 22 x 150mm 5um 100A	SHARC 1	5 um	22.0 mm	150 mm	100 A	Column	£7,275.73
SH1-220.250.0510	SHARC 1 Column 22 x 250mm 5um 100A	SHARC 1	5 um	22.0 mm	250 mm	100 A	Column	£9,157.67
SH1-05.100.0310	SHARC 1 Column 0.5 x 100mm 3um 100A	SHARC 1	3 um	0.5 mm	100 mm	100 A	Column	£1,451.90
SH1-05.150.0310	SHARC 1 Column 0.5 x 150mm 3um 100A	SHARC 1	3 um	0.5 mm	150 mm	100 A	Column	£1,519.27

Features

Acetonitrile/methanol mixture has 2-3 times lower viscosity than water/methanol or water/acetonitrile mixtures allowing use of faster methods without increasing the working pressure
Combination of methanol and acetonitrile allows good solubility for almost any molecule with high or low polarity
Solvent system is suitable for preparative chromatography and low viscosity mobile phase allows higher throughput
Compatible with LC-MS
Isocratic methods can be used due to high selectivity of the column and this allows recycling of the mobile phase to minimise solvent consumption
Resolution of molecules of similar nature such as isomers, related impurities, products of oxidation or reduction can be achieved
Practically any molecule with a functional group which contains oxygen and nitrogen can be retained and separated from similar compounds
Guard columns connect directly to columns and do not require any additional holders

Specifications

	SHARC 1
Mode	SHARC (Specific Hydrogen-bond Adsorption Resolution Chromatography)
Phase	Hydrogen bond acceptor
Particle Type	Fully Porous
Particle Size	3 µm, 5 µm
Pore Size	100 Å
pH Range	No water used
Temperature Range	0 - 50°C
Pressure Limit	345 bar

Literature