GC Method Development
1 Day Course
Supplemented by a host of real world separation examples, tutorial and exercises to aid understanding, this course includes all the essential aspects of GC method development. More experienced chromatographers can use this course to cut method development lead times drastically, armed with a logical approach and a wealth of new knowledge on how to make the right decision during each aspect of development.
- We recommend that course numbers are limited to 20 so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
- When delivered on-site we can design the course material to suit your specific training needs
- Customisable written assessments are available if required
Contact [email protected] for more information.
Course Overview | Download course details »
This one day course provides a step-by step approach to method development for GC. Column dimensions, phase type, inlet type and operating conditions, detector settings and optimisation along with sample preparation regimes are all crucial and often baffling choices available to the chromatographer developing methods for GC analysis.
This course takes a logical and progressive approach to the subject and discusses the important ‘make or break’ choices. Tutorial exercises illustrate the effect of different inlet conditions, oven temperature programme, stationary phase types and detection settings on real samples.
Who is this course for
This course is designed for the more experienced chromatographer. Attendance to the Fundamental GC and GC Troubleshooting and Maintenance training courses is not compulsory, but can be advantageous.
Delegates should have a good knowledge of chromatography and experience as GC users. Some experience in method development is also recommended. A good grounding in chemistry will be beneficial.
What you will learn
- Setting method development objectives
- Sample preparation
- Inlet and flow rate parameters
- Choosing a column and temperature program
- Optimisation strategies
- Establishing method objectives
- Literature searching
- What is known?
- What needs to be known?
- Sample clean up
- Analyte extraction
- Solvent selection
- Optimising for sample type/application
Inlet, and Flow Rate Parameters
- The effect of split ratio on peak shape and quantitative accuracy
- Investigating oven initial temperature
- Conversion into a splitless method
- Optimising purge-on time
- Carrier gas choice and flow rate optimisation (van Deemter & Golay treatment)
Choosing a Column & Temperature
- Choosing the correct phase
- Effects of column geometry
- Solute-stationary phase interactions
- Isothermal vs. Gradient operation
- Theory and development of temperature gradients
- Measuring and optimising:
Capacity factor, Efficiency, Resolution, Selectivity
- Resolution equation
- Developing effective methods
- Example method development
Putting it all together!
- Developing a method for the separation of a complex mixture of compounds from scratch