GC Fundamentals

1 Day Course

Basics of the chromatographic process, sample preparation, inlet systems, column and detector selection are important topics covered to give the participant a thorough grounding in the technique. Instrument hardware is also covered with basic troubleshooting and maintenance tips as well as an introduction to chromatographic optimisation. This course is not instrument-specific.

 

  • We limit numbers to 20 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises 
  • When delivered on-site we can design the course material to suit your specific training needs
  • Customisable written assessments are available if required


Course Overview | Download course details »

For the less experienced chromatographer or those wishing to update their skills, this course covers the fundamentally important concepts in modern GC analysis. 

The different components of the gas chromatograph (gas cylinders and generators, injectors, column oven, detectors, etc) are fully explored, as well as the theory of chromatographic separation, temperature programming, injection techniques and fundamentals of data analysis.

Who is this course for

This course is for current users of GC, or for anyone interested in gaining a deeper understanding of the chromatographic separation of volatile compounds amenable to analysis by this technique.

Previous knowledge

No previous knowledge of chromatography is necessary, but knowledge of basic chemistry is beneficial.

What you will learn

  • Basics of the chromatography process
  • Sample preparation protocols
  • Sample introduction
  • Columns and temperature programming
  • Detectors
  • Measuring and optimising chromatographic parameters

 

Course Outline

Basics of the Chromatography Process

  • Retention mechanisms in GC
  • Temperature/retention relationships
  • Column theory
  • Stationary phase chemistries

Sample Preparation Protocols

  • Principles
  • Matrix elimination
  • Solvent considerations
  • Solvent and Solid Phase Extraction

Sample Introduction

  • Operating principles
  • Typical operating conditions
  • Optimisation
  • Split/splitless
  • Cool on-column
  • Headspace (on request)

Columns and Temperature Programming

  • Choosing the right phase
  • Column geometries explained
  • Phase types
  • Temperature effects
  • Band broadening (van Deemter & Golay treatment
  • Isothermal vs. Gradient operation

Detectors

  • Choosing the right detector
  • Operating principles and optimisation
  • Typical operating conditions
  • FID, ECD, GC/MS

Measuring and Optimising Chromatographic Parameters

  • Efficiency
  • Capacity factor
  • Selectivity
  • Resolution
  • Interdependence via the resolution equation