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HPLC for Biomolecules

1 Day

Comprehensive training covering the use of HPLC in support of the analysis of biomolecules

This intensive one day course provides an extensive overview of the use of HPLC in the analytical support of biomolecular proteins, particularly monoclonal antibodies (mAbs).

We provide a foundation in understanding proteins and an introduction on how they are produced using biotechnology. We then look at specific HPLC techniques used in the analysis of amino acids, peptides, intact and subunit proteins. This includes size exclusion chromatography, ion exchange chromatography, reversed phase chromatography, HILIC and HIC.

It is of particular use to delegates from a  small molecule analytical background looking to apply their experience to the analysis of biomolecules.

  • Note this training does not cover LC-MS

Example Training


Video Overview


Video Walk-through

  • Overview of the different types of biomolecules
  • Biotechnology and the production of proteins
  • SEC and aggregates
  • IEX for charge variancy
  • Reversed phase for multiple applications
  • HILIC and HIC


  • Amino Acids and Peptides
  • Primary, secondary, tertiary and quaternary protein structure
  • Post translational modification
  • Monoclonal antibodies
  • Antibody drug conjugates (ADC)
  • Oligonucleotides

Introduction to Biotechnology

  • Nucleotides, DNA, mRNA, tRNA
  • Transcription and translation
  • ICH Q6(B)

Size Exclusion Chromatography

  • Protein aggregation
  • SEC columns and mobile phases
  • Basic method development

Ion Exchange Chromatography

  • Charge variancy
  • IEX modes
  • pH and salt gradient

Reversed Phase Chromatography

  • Columns and mobile phases
  • Protein primary structure
  • Peptides
  • Derivatised amino acid analysis


  • Overview of technique
  • Glycan analysis
  • Un-derivatised amino acid analysis

Hydrophobic Interaction Chromatography (HIC)

  • Overview of technique
  • Common uses

This material is primarily aimed at analysts and organisations currently working with small molecules or performing limited testing on biomolecules. It is of limited value to analysts with high levels of experience with biomolecules.

Delegates should already have a good understanding of HPLC.

Understanding HPLC

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