This course provides the basic knowledge required for the interpretation of unknown spectra. Starting from first principles, it introduces the fundamental chemistry of mass spectrometry and builds up to an examination of the most frequently encountered fragmentation patterns. This course is not instrument-specific and is suitable for anyone involved in mass spectrometry, from the analysts that wants to obtain good spectral data to the lab manager who needs to understand spectral interpretation in order to validate that data.
We limit numbers to 12 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
When delivered on-site we can design the course material to suit your specific training needs
Customisable written assessments are available if required
A course designed to highlight the possibilities of GC analysis with mass spectral detection from a qualitative point of view. Systematically analyse a mass spectrum to elucidate the underlying structural information.
Who is this course for Users of GC-MS equipment, or anyone interested in structural elucidation by GC-MS.
Previous knowledge A good working knowledge of the technique is required and previous attendance to our level 1 or 2 GC-MS courses is recommended. A basic knowledge of organic chemistry is advantageous but not essential.
What you will learn
How to optimise instrument and method parameters to obtain high quality spectral data
Ionisation and fragmentation mechanisms in GC-MS
Logical evolution of an ‘Interpretation Toolkit’ with a multitude of practice spectra with worked solution on which to practise new skills
The fundamental spectral interpretation toolkit, and how to use it when confronted with the mass spectrum of an unknown
Review of the chemistry of ions, electrons and radicals and isotopes vital to understanding of molecular fragmentation and ionisation
The principles of isotopic normalisation
Principles of ionisation
Techniques and impact on ionisation and fragmentation of Electron Impact (EI) and Chemically Induced (CI) ionisation mechanisms
Homo and heterolytic fission
McLafferty rearrangement and ortho effects
Retro Diels-Alder reactions
Common ion series
Rings and double bonds
The nitrogen rule
Logical (illogical) neutral molecular losses
Logical (illogical) neutral fragment losses
At all points during the course, practice spectra are given to highlight and allow the student to learn the principles under investigation
The course ends with practice exercises on several spectra to confirm overall understanding