1 Day Course
Next course is: Tuesday 25th June 2019. Venue: Cambridge - Anatune Labs.
- We limit numbers to 20 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
- When delivered on-site we can design the course material to suit your specific training needs
- Customisable written assessments are available if required
Course Overview | Download course details »
The Atmospheric Pressure Interface (API) is the core element to the course with the principles of operation, limitations and applicability fully explored.
The course covers ion suppression, the use of Electrospray or APCI and MS-MS data acquisition modes. Optimisation of interface and mass filter settings and how to best utilise reduced dimension LC to speed up sample throughput will be discussed.
All popular interface types and mass analysing equipment (quadrupole, TOF, ion trap, etc.) are comprehensively covered.
Who is this course for
Users of LC-MS and LC-MS-MS equipment, or anyone interested in learning the analytical capabilities afforded by this type of instrument.
No previous knowledge of mass spectrometry is necessary, but an understanding of the fundamentals of liquid chromatography is beneficial.
What you will learn
- How modern API designs work and which one best fits your LC and application requirements
- How ions are produced and how to optimise LC mobile phase and interface parameters to achieve highest sensitivity, accuracy and reproducibility
- The anatomy and working principles of quadrupoles, ion traps, magnetic sector, time-of-flight detectors and more
- The information that can be acquired in MS, MS-MS and MSn modes, and how to optimise instrument settings for high data quality
- Isocratic or gradient elution?
- Novel Column Geometries
- Stationary phase choice
- Column switching & fast LC
Sensitivity and Throughput
- Choosing the correct flow rate
- Flow splitting
- Common & Unsuitable mobile phase buffers
- Increasing Source Tolerance
- Concentration Dependence
- The API source
- APCI - principles & practice
- Electrospray - principles & practice
- Source maintenance
- Useful application examples
- LC-MS Data Acquisition Modes (sensitivity vs. specificity)
- Scanning vs. SIM
- Singly & multiply charged species
- Cone voltage fragmentation (Up-front CID)
LC-MS/MS Data Acquisition Modes
- Product ion scanning
- Precursor ion scanning
- Choosing precursor ions
- Constant neutral loss
- Data-dependent scanning
- Introduction to MS interpretation
- Establishing MRM method parameters