GC-MS training for the Chromatographer

1 Day Course

We have distilled critical knowledge of Gas Chromatography and Mass Spectrometry applications and instrumentation into this exciting and interactive short course. You will learn the absolute essentials of the technique and, using our unique multi-media examples and tutorial exercises, you will immediately enjoy the benefits on your return to your laboratory.

  

  • We limit numbers to 20 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises 
  • When delivered on-site we can design the course material to suit your specific training needs
  • Customisable written assessments are available if required


Course Overview | Download course details »

A course designed to highlight the powerful possibilities of GC analysis with mass spectral detection. Tuning and tune reports will be explained and instruction given in the use of tune reports as a powerful diagnostic tool. 

The functionality of the MS will be discussed in detail including principles of quadrupole and ion trap mass filters. Ion detection devices will be explained and the use of electron impact and chemical ionisation sources will be investigated with applicability to practical situations. GC Parameters will also be considered with operation and troubleshooting of inlets and columns explained.

Who is this course for
Users of GC-MS equipment, or anyone interested in learning the analytical capabilities afforded by this type of instruments.

Previous knowledge
No previous knowledge of mass spectrometry is necessary, but an understanding of the fundamentals of gas chromatography is beneficial.

What you will learn

  • How the different components of the mass spectrometer work with the gas chromatograph, from the transfer line to the vacuum pumps
  • How ions are produced, filtered and detected, and the alternative ways different instruments achieve this
  • How tuning affects the instrument and the data generated, and how to benefit from this knowledge
  • The rich plethora of information that can be acquired and how to optimise instrument settings for high data quality

 

Course Outline

Chromatographic Considerations

  • Sample preparation
  • Column configurations for GC-MS
  • Sample loading and stationary phase choice
  • Flow rate considerations

Sample Introduction

  • The transfer line
  • Flow splitting
  • The ion source explained
  • Modes of ionisation
  • Electron impact / chemically induced ionization, examples and fragmentation

MS Hardware

  • Why use vacuum?
  • Controlling and monitoring vacuum
  • Quadrupole mass analysis explained
    Ion traps explained

Detector Systems

  • Electron multipliers and detector electronics
  • Matthieu stability diagrams
  • X-ray lens and high energy dynodes
  • AMU gain and offset
  • Spectral resolution

Tuning and Calibration

  • Purpose of tuning
  • Tuning compoundsExplanation of auto-tune voltages
  • Troubleshooting from the auto-tune
  • User tuning and voltage ramping

Quantitation

  • Scan & SIM modes
  • High sensitivity data acquisition