Interpretation of LC-MS Data - New Course
2 x 1 Day Courses
This is taught online as two separate one days courses: level 1 and level 2. They provide a greater understanding of how to interpret the mass spectra on any instrument. Attendees will gain a solid grounding in this aspect of LC-MS analysis and be able to apply their knowledge practically in the lab.
- Level 1 - Chromatographic determination and molecular ion
- Level 2 - Fragmentation and Structural Determination
- Format - These are online courses, delivered live, and led by one of our trainers
- Duration - Each course consists of 2 x 4-hour session delivered over 2 consecutive days (total of 8 hours per course)
- We limit numbers to 8 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
Course Overview | Download course details »
Level 1
Here we explore acquisition and interpretation techniques that maximise the information you can obtain from even simple single stage (e.g single quadrupole) mass spectrometers. In this level we cover :
Highlighting peaks of interest from the chromatogram
- Using collision-induced dissociation effectively
- Comparison of CID and MS/MS spectra
- Using precursor ion scanning, constant neutral loss scanning and data-dependent experiments in tandem instruments
- Improving the quality of data generated in TIC
- Use anticipated mass differences (e.g. metabolic transformations) to highlight low concentration compounds of interest
- Using contour maps
- Using extracted mass effectively
Identification and confirmation of the molecular ion
- Background signals
- Multiple charging
- Adducts
- Influence of fragments of molecular ion confirmation
Building on the molecular ion
- Isotopes
- Rings and double bond equivalents
- Optimising empirical formula information
- Mass delta
Level 2
Here we explore the techniques used when working with fragments and applying these to structural determination
Revision of bonding theory
- Covalent bonds
- Lone pairs
- Bond location references
Ion formation
- Functional groups
- Charge site initiation
- Charge site stabilisation
Common fragmentation pathways
- Charge migration mechanisms
- Charge retention mechanisms
Using fragmentation for structural determination
All sections are extensively supported by workshops to illustrate and practice the techniques being learned.