Interpretation of LC-MS Data - New Course

2 x 1 Day Courses

This is taught online as two separate one days courses: level 1 and level 2. They provide a greater understanding of how to interpret the mass spectra on any instrument. Attendees will gain a solid grounding in this aspect of LC-MS analysis and be able to apply their knowledge practically in the lab.

Level 1 - Chromatographic determination and molecular ion
Level 2 - Fragmentation and Structural Determination

Format - These are online courses, delivered live, and led by one of our trainers
Duration - Each course consists of 2 x 4-hour session delivered over 2 consecutive days (total of 8 hours per course)
We limit numbers to 8 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises

Course Overview | Download course details »

Level 1

Here we explore acquisition and interpretation techniques that maximise the information you can obtain from even simple single stage (e.g single quadrupole) mass spectrometers. In this level we cover :

Highlighting peaks of interest from the chromatogram

Using collision-induced dissociation effectively
Comparison of CID and MS/MS spectra
Using precursor ion scanning, constant neutral loss scanning and data-dependent experiments in tandem instruments
Improving the quality of data generated in TIC
Use anticipated mass differences (e.g. metabolic transformations) to highlight low concentration compounds of interest
Using contour maps
Using extracted mass effectively

Identification and confirmation of the molecular ion

Background signals
Multiple charging
Adducts
Influence of fragments of molecular ion confirmation

Building on the molecular ion

Isotopes
Rings and double bond equivalents
Optimising empirical formula information
Mass delta

Level 2

Here we explore the techniques used when working with fragments and applying these to structural determination

Revision of bonding theory

Covalent bonds
Lone pairs
Bond location references

Ion formation

Functional groups
Charge site initiation
Charge site stabilisation

Common fragmentation pathways

Charge migration mechanisms
Charge retention mechanisms

Using fragmentation for structural determination

All sections are extensively supported by workshops to illustrate and practice the techniques being learned.