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Calibration and Quantitation Training Course

This one day course covers the most common methods used in calculation, calibration and quantitation of data obtained from instrumental analysis.

The material is extensively workshop driven, building your understanding of the different methods and when they would be applied. You will gain confidence in working with all types of calculated data.

  • Course delivered face-to-face in one day or online in two half-days
  • We limit numbers to 10 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
  • When delivered on-site we can design the course material to suit your specific training needs

 Who is this course for

This course is relevant to anyone involved in the generation or review of data associated with instrumental analytical chemical techniques such as HPLC, GC, mass spec, molecular and atomic spectroscopy.

Previous knowledge

Good knowledge of chromatography and associated data.


 Course Outline


  • Preparative challenges
  • Standard verification
  • Salt correction factor

Basic Proportional Calculations

  • Proportion
  • Beer's Law
  • Two point linear regression
  • Multipoint standard calibration
  • Bracketing

Internal Standards

Linear Calibration Lines

  • Correlation co-efficient
  • Residuals
  • Confidence Intervals
  • Outliers
  • Residual sum of squares
  • Use of the origin

Sample Purity

  • Percent area normalisation
  • Relative response factor

Verification of calibration performance

  • QCs and check standards
  • Surrogates

Non-linear calibration

Line weighting

  • Ordinary least squares regression
  • Inverse weighting
  • When do we use modified weighting?

System Suitability

  • Placebo and blanks
  • Signal to noise
  • Calibration verification
  • In sequence checks
  • Precision
  • Chromatographic peak shape and resolution

Standard Addition Method

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