Solid Phase Extraction

1 Day Course

Sample preparation training can help you learn or brush up on solid phase extraction, not matter your level of experience. Learn the absolute essentials of SPE and apply this knowledge for the betterment of your lab workflow.

We limit numbers to 20 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
When delivered on-site we can design the course material to suit your specific training needs
Customisable written assessments are available if required

Course Overview

A comprehensive one day course designed to increase expertise and optimise method development time for all users of solid phase extraction as a means of sample preparation.

The course is backed with a host of explanatory images and multimedia material from our range of e-learning products. Practical examples are included to reinforce the learning experience.

Who is this course for

This course is for LC or GC analysts or method developers who need to master the principles of Solid Phase Extraction in order to design and implement effective SPE protocols.

Previous knowledge

Knowledge of chromatography and a basic grounding in chemistry are beneficial. Previous experience using SPE will be also be advantageous.

What you will learn

The basics of SPE and practical considerations
Sorbent Selection
What are the various extraction techniques offered by SPE
Method development and protocol troubleshooting

Course Outline

SPE Sorbent Substrates

Protocol Steps in SPE

SPE Sample Pre-treatment
SPE Column Conditioning
SPE Column Equilibration
Sample Loading
Column Washing
Analyte Elution

Molecular Properties

Hydrophobic or Non-Polar Groups
Polar Groups
Ionic Groups
Chelating Groups

SPE Mechanisms

Non-polar SPE
Polar SPE
Cation-Exchange SPE
Anion-Exchange SPE
Mixed-Mode SPE

SPE Method Development

Analyte Assessment
Mechanism Selection
Sorbent Screening
Procedure Optimization